SRR3557427.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3557427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1614333
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26128	1.618501263370073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12683	0.7856495530971616	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9897	0.6130705374913354	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4848	0.3003097873858739	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3994	0.24740868209966593	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3949	0.24462115313259408	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC	3456	0.21408222467111804	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3061	0.1896139148490429	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC	2991	0.18527775867804225	No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA	2893	0.17920714003864133	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT	2839	0.17586210527815513	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	2524	0.15634940250865217	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2320	0.14371260452459314	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2133	0.13212887303920567	No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA	2115	0.13101386145237692	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT	1972	0.12215571384590417	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	1962	0.12153626296433265	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1847	0.11441257782626013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1846	0.11435063273810299	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	1819	0.11267811535785988	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	1790	0.11088170780130246	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	1768	0.10951891586184512	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT	1745	0.10809417883423061	No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1673	0.10363413248691564	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TACAACG	365	73.3831	2
ACAACGC	440	62.22739	3
TAACGCA	510	49.017994	4
GGACCGT	585	47.79135	6
CGCATAG	570	46.990932	1
GTATAAC	490	44.94507	1
GACCGTT	375	44.41545	7
ATAACGC	550	44.370834	3
AGATTAC	955	43.005314	2
GTACAAC	675	41.444843	1
AGTGTAC	835	39.918846	3
ATAGTAC	710	39.401787	4
GATTACT	1040	38.918133	3
TCTAACG	185	38.60877	2
TTCAACG	1025	37.164864	4
ATCTACG	295	36.318417	2
CATGGGT	1890	36.217	4
AACGCAG	28210	35.973545	6
CATGGGG	6170	35.404408	4
TACGCAG	410	34.84098	5

Summary