FastQCFastQC Report
Fri 10 Feb 2017
SRR3557426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4359783
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537161.232079670020274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245020.5620004481874442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202720.46497727065773686No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91630.21017101080489559No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65800.15092494282398916No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA65670.1506267628457655No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61710.14154374197064395No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC60320.13835550989579068No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58210.13351581948000624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51160.11734528989172166No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA47860.1097761058291204No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT47220.1083081428594038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8800.041.2546541
GTATCAA523150.040.8862841
AACGCAG543950.039.872576
CATGGGT91550.035.5586134
CGCAGAG615500.035.324498
ACGCAGA613950.035.2973987
TATCAAC606050.035.2436452
ATCAACG613300.034.7105753
TCAACGC627850.034.181074
CAACGCA634200.033.800135
GCAGAGT649450.033.4504249
ACATGGG538100.031.7309723
TACAACG7550.031.5303572
ATGGGTG50350.031.4400355
GTACATG560500.031.2917821
TACATGG561750.031.1472762
GTACTGG29700.030.2584231
CATGGGG149150.029.9263554
ATAGTAC13000.028.8411794
TAACGCA8100.028.654674