FastQCFastQC Report
Fri 10 Feb 2017
SRR3557425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037021
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232661.1421580828081792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108160.5309714529207111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93620.4595927091571466No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42690.2095707408023776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41870.20554525456536776No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC22080.10839358062582566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.10692084175862693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5800.047.205051
AACGCAG209050.041.687026
GTATCAA226850.036.942181
GTACATG212250.036.8753971
TAACGCA4700.036.7238434
TACATGG214600.036.4706952
ACGCAGA239950.036.194747
CGCAGAG242100.036.020748
ACATGGG215250.035.8905033
TATCACG2550.035.010562
ACAACGC3950.034.6560253
GCAGAGT256850.033.952199
TCTAACG1953.8198777E-1133.574232
CATGGGG61950.033.049524
ATCAACG250500.032.954653
AGATTAC11750.032.9248242
TATCAAC255400.032.5321352
TCAACGC257700.032.4958344
CTAACGC2203.6379788E-1232.464343
CAACGCA261800.032.2589385
Produced by FastQC (version 0.11.5)