FastQCFastQC Report
Fri 10 Feb 2017
SRR3557422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9919008
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT827130.8338837916049671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419360.4227842139052615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296160.29857824492126633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145780.1469703421955099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA139580.14071971713300363No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT127210.128248711967971No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC102590.1034276814778252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA844450.037.592431
AACGCAG894250.036.3262336
ACGCAGA996950.032.4886477
CGCAGAG1007600.032.1567348
ATCAACG997950.031.5476883
TATCAAC1004500.031.5256792
TCAACGC1014900.031.3844454
CAACGCA1021250.031.3101235
GCAGAGT1068100.030.3575699
CATGGGG286150.030.2047754
ACATGGG876500.030.0445693
GTACATG924250.029.5357061
TACATGG927250.029.375952
CATGGGT113250.028.0150724
AGAGTAC1046050.025.33935210-11
CGCATAG17050.025.1368521
AGTACTT551950.024.97599212-13
GTACTGG61800.024.3688351
TAACGCA14900.023.9699864
CATGGGC215050.023.638574
Produced by FastQC (version 0.11.5)