FastQCFastQC Report
Fri 10 Feb 2017
SRR3557421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4102313
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387550.944710947214413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191820.46758986942244535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143700.35029018994893857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.17424316477070376No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55550.13541141302479845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44760.10910917816363598No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41670.10157684213759409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10150.040.466551
GTATCAA395000.036.7710341
AACGCAG414100.036.2855836
TAACGCA9750.032.3574144
ACGCAGA463900.032.249257
CGCAGAG468250.032.08948
ATCAACG452750.031.7512783
TATCAAC455300.031.6649652
ACATGGG416800.031.6478793
TCAACGC463900.031.38594
GTACATG436900.031.2689861
CAACGCA470150.031.1820075
TACATGG437800.031.0543842
GCAGAGT492250.030.5490269
ACAACGC8500.030.1128653
CATGGGG117100.029.2798024
ATAGTAC14300.029.138324
TACAACG8450.028.882162
CATGGGC100750.027.7686884
TATCACG6450.027.6862472
Produced by FastQC (version 0.11.5)