FastQCFastQC Report
Fri 10 Feb 2017
SRR3557420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340943
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235831.0074145333739437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120390.5142799290713187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95820.4093222261285303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58290.24900221833679845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43930.18765941759367913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.18624972927576622No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC34330.14665030289075812No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31270.13357864757920207No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29730.12700010209560847No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT29700.12687194861216183No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA24660.10534216339312832No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT23570.10068592016123418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6050.045.2546841
TACAACG4650.040.9598242
TAACGCA5750.040.3699154
ATAGTAC7700.036.3302354
CATGGGT37950.034.8178754
TATCACG3250.034.796162
CATGGGG74450.034.1369134
ATCTACG4300.033.2203252
ACATGGG289000.033.0138933
GTACATG311000.032.209561
TACATGG310500.032.069742
AACGCAG295050.031.8142686
ACAACGC5800.031.8122793
CATGGGC60200.030.5508354
GTATAAC8000.030.503871
GTATCAA292550.030.4770411
GGACCGT5700.030.2645156
AAACGCA8100.030.1259925
TAGTACT9200.029.75865
GACCGTT3700.028.9388527
Produced by FastQC (version 0.11.5)