FastQCFastQC Report
Fri 10 Feb 2017
SRR3557418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455642
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361991.474115526611778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184420.7510052361052629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134760.5487770611514219No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73220.2981704987942054No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58800.23944858411771747No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56080.2283720509748571No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46580.18968563007148437No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36660.14928886213869938No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA35920.14627539356306823No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC34280.1395968956386965No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC33950.13825305154415832No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30990.12619917724163376No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30750.1252218360819696No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29300.11931706657566535No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.10999160301053655No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA25340.10319093744120683No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA24810.10103264238028181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8550.058.4756771
TATCACG4150.048.761292
ATAGTAC11000.045.44974
AGATTAC11550.044.316032
TCACGCA4950.043.2854234
GCATAGT12200.040.9792332
TAACGCA6400.039.9850355
AGTGTAC11700.039.6783073
TAGTACT12750.038.7415435
ACAACGC5900.038.333283
AAACGCA10600.037.055025
GATTACT14150.036.1731573
CATGGGT36900.035.9685444
TACAACG6000.035.7104762
CATGGGG79550.035.1643074
CATAGTA14450.035.010273
AACGCAG398850.034.8854146
ATGGGTG19650.034.2235345
ACGGTAC5750.034.1578453
GTGTACT14550.032.7243774