FastQCFastQC Report
Fri 10 Feb 2017
SRR3557413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7273782
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT715650.9838760633739093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391360.5380419704632335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288840.3970974109479773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130670.17964519695531156No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC95330.13105974306076262No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94010.1292450062429696No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT79120.10877422501801677No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT77100.10599712776654567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA753300.035.8287621
AACGCAG771300.035.609736
CGCATAG14450.033.8027271
ACGCAGA861500.031.8261137
CGCAGAG867500.031.6608478
TATCAAC881100.030.4599932
ATCAACG880250.030.408213
TCAACGC896750.030.240584
GCAGAGT916350.030.0515529
CAACGCA904950.030.0375165
TAACGCA13050.029.6665254
ACGGTAC17100.029.2581633
GTACTGG51750.027.2799591
ACATGGG696150.026.8139023
CATGGGG208900.026.3734634
TACTGGT49300.026.3374352
GTACATG745900.026.2736851
CATGGGT86750.026.2275524
TATCACG11150.026.1749022
TACATGG748300.026.123222
Produced by FastQC (version 0.11.5)