FastQCFastQC Report
Fri 10 Feb 2017
SRR3557412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338309
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT639571.198076019953135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324810.608451103148956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243890.45686752115698065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117850.22076279211263342No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA104860.19642924379236945No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC97630.18288562913836573No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90280.16911722419964825No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT88880.16649467087798778No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC85080.1593763118620522No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63360.11868927032886256No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT62160.11644136748172502No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60850.11398740687359986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54740.1025418348769245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10500.043.6810461
GTATCAA675200.037.4222451
AACGCAG706450.036.605836
TAACGCA10900.033.8794564
CATGGGT83600.032.915994
ACGCAGA784150.032.8727267
CGCAGAG788300.032.7977758
TATCAAC785050.031.9643142
ATCAACG792200.031.5855963
GCAGAGT826400.031.336959
TCAACGC812300.031.2806384
CAACGCA816800.031.216815
GTACTGG46250.030.265491
ACATGGG667250.029.582523
TACTGGT43250.029.4712732
CATGGGG188200.029.4330024
ACGGTAC15600.029.3993243
GTACATG706950.029.0094761
TACATGG707100.028.9261532
ACAACGC9700.028.2460233