FastQCFastQC Report
Fri 10 Feb 2017
SRR3557410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6377264
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT643901.0096806404752883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330700.5185609377312904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248440.3895714525853093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118390.18564387486545955No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC116120.18208435466996506No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT110270.1729111418313559No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC106810.1674856176567255No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89580.14046776172352282No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78690.12339147320857345No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT73090.1146102780126399No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA67870.10642494963357327No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT66840.10480983694574977No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA64250.10074853416763051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA654700.039.4332121
AACGCAG693100.038.066626
CGCATAG11050.035.579291
ACGCAGA778150.033.7837077
CGCAGAG786200.033.4983258
TATCAAC771150.033.3842052
ATCAACG776550.033.044673
TCAACGC797350.032.593534
CAACGCA802400.032.492075
GCAGAGT826400.031.854669
GGACCGT17300.031.636496
ACGGTAC17900.031.6130033
CATGGGT65600.030.6907844
TCACGCA11150.030.450554
ACATGGG654650.030.1353533
CATGGGG202300.029.9741654
GTACATG689150.029.7344761
TACATGG689400.029.6704542
TAACGCA9650.029.6284624
GTACTGG55550.029.5965181
Produced by FastQC (version 0.11.5)