FastQCFastQC Report
Fri 10 Feb 2017
SRR3557409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143398
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444401.0725496319687369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228790.5521796361343998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172190.41557678021758954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80230.19363334152306874No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC68670.1657335356149711No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC67890.16385102275958044No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT67200.1621857229259656No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56900.1373268993227298No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT49170.1186707142302043No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA47270.11408510599271418No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46720.11275769308186179No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT44940.1084617022067395No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA43210.10428638523260376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8150.040.920811
GTATCAA416750.039.641011
AACGCAG438550.038.525336
ACGCAGA496550.033.9299057
CGCAGAG500150.033.816538
TATCAAC489000.033.6723862
ATCAACG489650.033.445263
TCAACGC503650.033.2250064
CAACGCA508050.033.018515
GTACTGG33450.032.937411
GCAGAGT524850.032.2594769
ACATGGG418000.032.126093
GTACATG434450.031.912221
TACATGG436450.031.6958432
ACGGTAC10100.030.6598173
TACTGGT31200.030.5388722
CATGGGG134550.030.4060964
GGACCGT10150.029.8917476
CATGGGC98350.029.6088874
GGTTCAC32600.028.8327886
Produced by FastQC (version 0.11.5)