FastQCFastQC Report
Fri 10 Feb 2017
SRR3557407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897178
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282610.9754664711660797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139500.48150303502235625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114650.3957299137298433No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55700.19225605054297665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.1725472166363268No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47760.16485007134528842No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44980.15525452699143788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44940.15511646160505155No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC40230.13885926235806015No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT31140.10748390330176467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5550.054.7344781
ACGGTAC7050.038.018253
GTATCAA328500.035.7021261
AACGCAG342050.035.4452746
TACAACG5600.034.0353852
TAACGCA6150.032.9285474
ATAGTAC9650.032.0955474
ACGCAGA381200.031.664527
CGCAGAG384250.031.5834888
ACAACGC5950.031.0322653
GTACTGG24800.030.9828821
ATCAACG377550.030.9207733
TATCAAC378900.030.8734842
TCAACGC387100.030.6810864
CAACGCA390400.030.4964475
TAGTACT10550.030.4850965
CATGGGT36450.030.3937424
GCAGAGT402950.030.1182889
TACTGGT23100.029.9098852
TATCACG4600.029.7809642