FastQCFastQC Report
Fri 10 Feb 2017
SRR3557396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4497823
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT506871.1269229580621558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249200.5540458128298957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196190.43618879622430673No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC98950.21999531773482417No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT90180.20049699599117174No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC88380.19649505994344374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87870.19536117806325418No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT66230.1472490135783467No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63900.14206872969434325No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA60360.13419825546714487No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT59070.1313302012996065No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58820.13077437684853316No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA51130.11367721673351754No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT50760.11285459654592898No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA47800.10627363504522078No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC47530.10567334463806156No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46380.10311655216312425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10100.041.876641
GTATCAA492850.039.8268051
AACGCAG517700.038.8859566
ACGCAGA584200.034.3584677
CGCAGAG588150.034.1884048
TATCAAC574600.033.9820182
ATAGTAC13050.033.7774624
ATCAACG579600.033.534713
TCAACGC593200.033.3181654
CAACGCA598600.033.1440125
GCAGAGT620250.032.4293569
TAACGCA6800.032.4114654
GTACTGG42600.030.9042021
TACTGGT37000.030.749462
ACATGGG487700.030.2415263
AGATTAC17800.030.1181552
CATGGGT46900.030.101034
ACGGTAC13300.030.007443
GTACATG515400.029.5890081
TACATGG516950.029.4522292
Produced by FastQC (version 0.11.5)