FastQCFastQC Report
Fri 10 Feb 2017
SRR3557395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481857
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381861.0967136214956559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186060.5343700215143816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151560.43528496431645525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69700.20018053584624526No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62130.17843926387556983No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60010.17235055891152334No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55160.15842121029094533No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54020.1551470953574486No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53120.15256226777837228No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT37630.10807451311182509No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35810.10284741734080406No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.10281869703436988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6000.050.6266481
TAACGCA5800.041.0746154
GTATCAA416750.037.4014361
AACGCAG433900.036.922856
ACGCAGA482900.033.0407647
CGCAGAG485700.032.8747868
ACAACGC6550.032.7342723
TATCAAC483150.032.0996632
ATCAACG486300.031.8672433
TCAACGC499100.031.5630954
CAACGCA502000.031.402685
GCAGAGT511800.031.1982869
TACAACG6500.031.1535152
TATCACG5150.030.0682132
CATGGGG121850.029.864634
CATGGGT43550.029.6765274
ATAGTAC10700.029.5007864
GTACTGG26150.029.1540221
AGATTAC13700.029.1269992
ACATGGG405800.028.5315723