FastQCFastQC Report
Fri 10 Feb 2017
SRR3557392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5030285
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT493950.9819523148290803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247410.4918409195502839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182990.3637766051028918No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC85630.17022892341090018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84910.16879759297932423No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT83580.16615360759877423No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC77900.15486200086078622No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61610.12247814984638049No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55300.10993412898076352No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT54810.10896002910371877No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA53150.10566001727536313No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT50960.10130638721265296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA471550.041.8459931
AACGCAG511200.039.322466
ACGCAGA571500.035.0485657
TATCAAC561550.034.978772
CGCAGAG577550.034.7741248
ATCAACG563300.034.711463
TCAACGC576950.034.2929424
CAACGCA578750.034.2765435
CGCATAG11400.033.4477161
GCAGAGT610350.032.925199
TAACGCA8200.031.9678084
GTACTGG42750.031.3572311
ACATGGG496000.030.628983
TACTGGT38900.030.4773712
GTACATG517400.030.2845171
TACATGG519900.030.1491492
CATGGGG167500.029.7704014
GGTTCAC40850.027.2328386
AGAGTAC600300.027.01005710-11
CATGGGT50500.026.6619014
Produced by FastQC (version 0.11.5)