FastQCFastQC Report
Fri 10 Feb 2017
SRR3557380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5693915
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582721.0234083227445439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295930.5197302734585957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212710.3735742454883854No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC118600.2082925368573293No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC116980.20544739427968278No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT111150.1952083935218562No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109280.19192418573160996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102970.18084217976559186No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT95370.16749459730255895No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT77470.13605752808041569No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT77020.1352672106977361No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA76520.13438908027253654No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT64290.11291001007215598No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA60980.10709678665733507No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT60400.1060781553641036No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC60170.10567421536851183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11100.039.177041
GTATCAA609100.038.2695121
CATGGGT85100.037.0996974
AACGCAG664150.036.0000846
CATGGGG198000.033.364954
TACTGGT44900.032.902542
GTACTGG48050.032.7297171
ACGCAGA739300.032.2199637
CGCAGAG742700.032.1525658
TATCAAC723600.032.147452
ATCAACG723500.031.9789923
TCAACGC739750.031.7113574
CAACGCA745500.031.5535555
ACATGGG647150.031.4163273
GTACATG694150.030.6713521
GCAGAGT779950.030.6251379
TACATGG694200.030.5742132
ACGGTAC14350.030.3036583
ATGGGTG48550.029.8143835
TATCACG8250.029.6042462