FastQCFastQC Report
Fri 10 Feb 2017
SRR3557373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246234
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195771.5708927857850132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108980.8744746171264787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89240.7160773979846481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.31013437283848777No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30030.24096598231150812No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT28660.22997286223935473No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.2040547762298252No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC21590.17324194332685514No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.1620883397499988No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT17860.14331176969975143No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.13641097899752375No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC16640.13352227591287028No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT16380.1314359903517317No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15490.12429447439244957No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA15000.12036262852722682No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14390.11546788163378627No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA14320.11490618936732587No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.1033513770287121No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA12780.10254895950519727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGGT11100.045.5581632
GGTTCAC12050.041.4710546
GTACTGG12300.041.1464841
ACTGGTT12300.040.6297763
TACAACG2050.037.7276462
AACGCAG176200.035.9580846
TACATGG173400.035.8196752
GTACATG173700.035.786531
ACATGGG170300.035.6332783
TGGTTCA14150.035.3163385
CATGGGG44250.034.956694
TCTAACG1408.074312E-833.996342
GTATCAA185350.033.7299421
CTGGTTC14750.033.4777534
TTCACTA14750.033.476418
GTTCACT14950.033.0285647
CATGGGC39750.032.6279374
TCACTAT15400.032.4497539
ATGGGCA16900.031.6817135
CGCAGAG201250.031.630118
Produced by FastQC (version 0.11.5)