FastQCFastQC Report
Fri 10 Feb 2017
SRR3557370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930752
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT676421.371839427332788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358840.7277591734485936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258340.5239363082953675No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129860.2633675350129149No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT90340.18321748893475073No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80120.16249042742364653No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC77480.1571362745479797No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC73340.14873998935659308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71070.1441362291188038No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.14330471295250705No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT58050.11773052061835598No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.10821878691120543No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT53320.10813766338278624No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA50850.10312828550290097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10250.048.794721
AACGCAG575400.041.1495366
GTATCAA560550.040.883771
ACGCAGA655750.036.0439157
GTACATG558250.035.964681
CGCAGAG660400.035.826168
TACATGG562800.035.5727922
ACATGGG552550.035.4574173
TATCAAC655600.034.8481142
ATCAACG662150.034.4225233
GCAGAGT694200.034.176089
TCAACGC678550.034.1429524
CATGGGT63400.033.783154
CAACGCA689200.033.7334675
ATAGTAC15600.033.18044
CATGGGG174300.032.3933454
TAACGCA10850.032.35264
ACGGTAC13250.032.329843
GTACTGG41100.032.3057941
TACTGGT38000.031.4701922
Produced by FastQC (version 0.11.5)