FastQCFastQC Report
Fri 10 Feb 2017
SRR3557369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757988
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338121.2259661753423148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189990.6888717427341962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138720.5029753573982193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69670.2526116864902965No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT61560.22320619234021322No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47050.17059537604949696No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA46890.17001524299598114No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46010.1668245112016441No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC42530.15420661728767493No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT39300.14249518126982422No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT39300.14249518126982422No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37390.1355698429434791No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35030.12701288040412068No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32360.11733191007357537No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA29960.10862991427083803No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28340.1027560671039903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6550.048.1721531
TACAACG5200.043.4752852
GTACTGG24900.041.601731
GTATAAC8600.041.5350071
TACTGGT23500.040.758692
TATCACG4700.039.239732
TAACGCA8000.038.6701244
ATAACGC9350.038.1773
GGTTCAC24800.037.180116
ACAACGC6150.036.759593
AACGCAG342750.035.9445726
ACATGGG339300.035.734073
GTACATG352900.035.5446621
TACATGG352850.035.5083432
CATGGGG84750.034.9584244
CATGGGT35850.034.683214
ACTGGTT27400.034.3058933
CATGGGC80150.034.2926184
GTATCAA341250.034.1063771
ATCTACG5600.033.9957122