FastQCFastQC Report
Fri 10 Feb 2017
SRR3557367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554576
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199881.2857525138687333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112720.7250851679171684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87480.5627257850372063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45450.29236267638249913No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.22507744877059724No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT34690.2231476621278085No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33270.21401333868527497No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC26020.16737682815121294No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC25630.16486810551558753No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT24050.15470456253023332No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT22010.14158201335926968No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.13978087915933346No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA20690.13309095213099906No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20370.1310325130453577No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.12678698243122238No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA17590.1131498234888484No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17240.11089840573892816No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT16680.1072961373390558No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC16040.10317925916777308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3450.058.6815451
TACAACG3150.054.7729032
TATCACG2700.044.0701562
TAACGCA3750.042.836194
ACAACGC4050.042.601153
GTATAAC3950.040.7013051
ACGGTAC4300.040.124343
TACTGGT12500.039.0285262
CATGGGT21600.038.8368234
TAGTACT5950.037.9929545
AACGCAG197100.037.7880176
ATAGTAC5250.037.396674
ACATGGG194100.037.3949243
GTACATG198650.037.1385081
ATGGGTG14100.037.1278655
TACATGG199200.036.975132
GTACTGG15300.035.4153631
ATGGGAC15550.034.8134775
GTATCAA207000.034.4610251
CATGGGG52250.034.1596374
Produced by FastQC (version 0.11.5)