FastQCFastQC Report
Fri 10 Feb 2017
SRR3557366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686091
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1549611.7840130848272253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT758570.8733157412235262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT584200.6725695137202684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259190.29839659750283526No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT189460.21811882928696003No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC178420.20540885422452976No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC157650.1814970623724757No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA149230.17180340385565843No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148550.17102054307282757No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT130710.15048196018208881No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA112390.12939076967994004No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107320.12355385178442178No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97060.11174186409053277No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA91630.10549049048645702No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT90910.10466157906934201No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT89370.10288862964940154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC88530.10192156632943404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1100750.049.4078181
AACGCAG1220850.045.1033976
TATCAAC1337000.040.657942
ATCAACG1361150.039.7835923
ACGCAGA1391950.039.486587
CGCAGAG1396450.039.3714378
TCAACGC1394300.039.208954
CAACGCA1413100.038.718765
GTACTGG79300.037.463051
GCAGAGT1468150.037.4567579
ACGGTAC28150.035.9288833
TACTGGT78600.035.726682
GGTTCAC82950.032.847126
GTACATG1113200.032.0567361
CATGGGT123050.031.9106374
TACATGG1119750.031.8310852
ACATGGG1103350.031.7596113
GGTATCA628850.031.620971
CGCATAG13200.031.571841
ACTGGTT89350.030.695843