FastQCFastQC Report
Fri 10 Feb 2017
SRR3557363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029909
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228941.1278338092988405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125310.6173183132839946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97540.4805141511269717No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.23848359704794647No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT32450.15985938285903456No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.15936674993805142No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA31320.15429263085192488No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29600.1458193446110146No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC25070.12350307329047755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.12108917197766009No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC23910.11778853140707293No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA22040.1085762957846879No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21010.10350217669856136No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT20880.10286175390128326No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20580.10138385513833378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5300.053.9091531
ATAGTAC7500.039.663584
TATCACG3500.039.0969542
AGATTAC8800.037.860692
TAACGCA4700.036.709914
AACGCAG246050.035.592376
TACAACG3950.034.642872
TAGTACT8600.034.5894785
CATGGGT26000.034.5530784
ACATGGG243650.034.2589763
GTATCAA247050.033.876431
CATGGGG71600.033.8192984
TACATGG254650.033.807112
GTACATG254650.033.7536471
GTATAAC5850.033.578031
AAACGCA5700.033.400095
GCATAGT8600.033.206722
GATTACT9950.032.8868873
ACGCAGA276550.031.7100057
CGCAGAG278000.031.630218
Produced by FastQC (version 0.11.5)