SRR3557359.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3557359.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4807588
Sequences flagged as poor quality	0
Sequence length	125
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74241	1.5442463039678107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40747	0.8475559885747281	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27704	0.5762557024437202	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14209	0.29555361233117317	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT	12708	0.2643321349499999	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC	12159	0.2529126871936614	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC	11698	0.24332367915054284	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10237	0.21293421982083324	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT	8519	0.1771990445104697	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8263	0.17187412898110238	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT	8165	0.16983568475501645	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8042	0.16727722924676575	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	7382	0.1535489313976156	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC	6540	0.13603495141430588	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT	6456	0.13428771350623223	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	6375	0.13260287695201836	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA	6327	0.13160445529026196	No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA	6244	0.1298780178334749	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5986	0.1245115014015344	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	5914	0.12301386890889986	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC	5802	0.12068421836480163	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5460	0.11357046402478749	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	5298	0.11020079091635972	No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA	5203	0.10822474804413357	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	5103	0.10614470291547444	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	5033	0.10468867132541308	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA	4847	0.10081978738610714	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	1220	43.919586	1
TACTGGT	4345	40.9406	2
GTACTGG	4680	40.453568	1
AACGCAG	68475	40.294796	6
GTATCAA	69275	38.544415	1
GTATAAC	1255	36.52772	1
TAACGCA	1195	35.84573	4
CGCAGAG	77240	35.760754	8
ACGCAGA	77205	35.707615	7
TACAACG	870	35.5596	2
ACTGGTT	5015	35.115074	3
AGATTAC	2020	34.75389	2
ACATGGG	63250	34.59585	3
GTACATG	65285	34.379814	1
TACATGG	65325	34.316605	2
GCAGAGT	80945	34.190067	9
CATGGGT	5720	33.90739	4
GGTTCAC	5125	33.89528	6
TATCAAC	78715	33.78492	2
ATGGGAC	5020	33.537674	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content