##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557353.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1141452 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57782105598834 33.0 33.0 34.0 32.0 34.0 2 32.55530937788011 33.0 33.0 34.0 31.0 34.0 3 32.633323170838544 33.0 33.0 34.0 31.0 34.0 4 32.5409171826761 33.0 33.0 34.0 31.0 34.0 5 32.640773330810234 34.0 33.0 34.0 32.0 34.0 6 36.05007131267894 38.0 37.0 38.0 31.0 38.0 7 36.200774101758114 38.0 37.0 38.0 33.0 38.0 8 36.39170022042101 38.0 37.0 38.0 33.0 38.0 9 36.48630516219692 38.0 38.0 38.0 34.0 38.0 10-11 36.47767054593623 38.0 38.0 38.0 34.0 38.0 12-13 36.47248548340184 38.0 38.0 38.0 33.5 38.0 14-15 36.54457392864527 38.0 38.0 38.0 34.0 38.0 16-17 36.47968727550523 38.0 38.0 38.0 34.0 38.0 18-19 36.55860255183748 38.0 38.0 38.0 34.0 38.0 20-21 36.54716492677747 38.0 38.0 38.0 34.0 38.0 22-23 36.506121588993665 38.0 38.0 38.0 34.0 38.0 24-25 36.497505808391416 38.0 38.0 38.0 34.0 38.0 26-27 36.44031198858997 38.0 38.0 38.0 34.0 38.0 28-29 36.439629524500376 38.0 38.0 38.0 34.0 38.0 30-31 36.360122896100755 38.0 38.0 38.0 33.0 38.0 32-33 36.16778322697757 38.0 38.0 38.0 33.0 38.0 34-35 35.94673494811871 38.0 37.5 38.0 31.5 38.0 36-37 35.75578955575881 38.0 37.0 38.0 30.0 38.0 38-39 35.49990144132211 38.0 37.0 38.0 28.5 38.0 40-41 35.22734114093278 38.0 37.0 38.0 26.5 38.0 42-43 34.880059345465256 38.0 36.5 38.0 24.5 38.0 44-45 34.44063219478349 38.0 36.0 38.0 16.0 38.0 46-47 33.87677712247208 38.0 35.0 38.0 15.0 38.0 48-49 33.35338542487989 38.0 34.0 38.0 15.0 38.0 50-51 32.91272388151232 38.0 32.5 38.0 15.0 38.0 52-53 32.60496017353336 38.0 30.0 38.0 15.0 38.0 54-55 32.27436852359976 38.0 27.5 38.0 15.0 38.0 56-57 32.05161101824693 38.0 26.0 38.0 15.0 38.0 58-59 31.86225570589039 38.0 26.0 38.0 15.0 38.0 60-61 31.739492330820745 38.0 25.0 38.0 15.0 38.0 62-63 31.60189346551585 38.0 25.0 38.0 15.0 38.0 64-65 31.4909050052039 38.0 25.0 38.0 15.0 38.0 66-67 31.333760858976113 38.0 24.5 38.0 15.0 38.0 68-69 31.196053798144817 38.0 24.0 38.0 14.0 38.0 70-71 31.03436938215536 38.0 24.0 38.0 14.0 38.0 72-73 30.883223298044946 38.0 24.0 38.0 14.0 38.0 74-75 30.783198943100544 38.0 24.0 38.0 14.0 38.0 76-77 30.591682786485983 38.0 18.5 38.0 14.0 38.0 78-79 30.475624029744573 38.0 16.0 38.0 14.0 38.0 80-81 30.340582433602115 38.0 15.0 38.0 14.0 38.0 82-83 30.262453874538746 38.0 15.0 38.0 13.5 38.0 84-85 30.20652992854715 38.0 15.0 38.0 13.5 38.0 86-87 30.168007940763168 38.0 15.0 38.0 13.0 38.0 88-89 30.064492856467027 38.0 15.0 38.0 13.0 38.0 90-91 29.989830058556997 38.0 15.0 38.0 13.0 38.0 92-93 29.925864600526346 38.0 15.0 38.0 13.0 38.0 94-95 29.871497443606913 38.0 15.0 38.0 13.0 38.0 96-97 29.79615919022438 38.0 15.0 38.0 13.0 38.0 98-99 29.73688994368576 38.0 14.0 38.0 13.0 38.0 100-101 29.66116315009304 38.0 14.0 38.0 13.0 38.0 102-103 29.56957147562929 37.5 14.0 38.0 13.0 38.0 104-105 29.505842996464153 37.0 14.0 38.0 13.0 38.0 106-107 29.413280190494213 37.0 14.0 38.0 13.0 38.0 108-109 29.325318979685527 37.0 14.0 38.0 13.0 38.0 110-111 29.225645055595855 36.5 14.0 38.0 13.0 38.0 112-113 29.16064451242803 36.0 14.0 38.0 13.0 38.0 114-115 29.08427949664112 36.0 14.0 38.0 13.0 38.0 116-117 28.961733826739977 36.0 14.0 38.0 13.0 38.0 118-119 28.82815834568602 35.5 14.0 38.0 13.0 38.0 120-121 28.77944276237634 35.0 14.0 38.0 13.0 38.0 122-123 28.72607915181716 35.0 14.0 38.0 13.0 38.0 124-125 27.5501952775938 32.0 14.0 38.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.014212037322245408 1101 2 0.19769368624925931 1101 3 0.0323485157508685 1101 4 -0.07593221906508774 1101 5 -0.12683717798977767 1101 6 0.04311057924901718 1101 7 0.11185045609676081 1101 8 0.06525852716765002 1101 9 0.1379081785506031 1101 10-11 0.09309232791712674 1101 12-13 0.04350962676272019 1101 14-15 -0.08634413075116498 1101 16-17 -0.0021103403683753186 1101 18-19 0.17992796328281457 1101 20-21 0.10809428007767252 1101 22-23 0.013594855950572082 1101 24-25 0.047403786408537485 1101 26-27 0.1381022937784806 1101 28-29 0.03757631015714935 1101 30-31 -0.052122300513261166 1101 32-33 -0.09191224236551676 1101 34-35 -0.2159380339126855 1101 36-37 -0.3235473191230156 1101 38-39 -0.047163601270163724 1101 40-41 -0.15076642271114338 1101 42-43 -5.024176054604368E-4 1101 44-45 -0.013804312476921154 1101 46-47 -0.06800908119687676 1101 48-49 0.030214123044295604 1101 50-51 -0.04601486808403621 1101 52-53 -0.10298897799907536 1101 54-55 -0.02195486800328439 1101 56-57 0.28952074985314624 1101 58-59 -0.09130240039349147 1101 60-61 0.1278662402458579 1101 62-63 0.07125418937308936 1101 64-65 0.0546088810071339 1101 66-67 -0.021004155576125783 1101 68-69 0.06944292208225633 1101 70-71 -0.20827788839382322 1101 72-73 -0.1390825727301923 1101 74-75 -0.17617416386910278 1101 76-77 -0.114837407996351 1101 78-79 -0.03612942656493345 1101 80-81 -0.15748163134475135 1101 82-83 -0.1457202006693734 1101 84-85 0.013337636797107422 1101 86-87 0.07770765559125792 1101 88-89 -0.15028560041504235 1101 90-91 -0.14700266515718852 1101 92-93 -0.061020230715381985 1101 94-95 -0.12225351600254797 1101 96-97 -0.07324830760707002 1101 98-99 -0.029286631472043467 1101 100-101 -0.03385073925381121 1101 102-103 -0.22082163243192454 1101 104-105 -0.17934931146256616 1101 106-107 -0.19763382041390898 1101 108-109 -0.15439283140047166 1101 110-111 -0.051968358453439834 1101 112-113 -0.01073200607276803 1101 114-115 -0.2230100845210714 1101 116-117 -0.16431679806249377 1101 118-119 -0.3004686385549853 1101 120-121 -0.20786659732289436 1101 122-123 -0.55757010575525 1101 124-125 -0.7415745897583825 1102 1 -0.0024166983787807794 1102 2 -0.004109032802062984 1102 3 0.011078227675163532 1102 4 0.282392629710138 1102 5 0.11101093843832643 1102 6 -0.03340017118519256 1102 7 -0.6286568206595362 1102 8 -0.06927761704987034 1102 9 0.08446444614358484 1102 10-11 0.06683311967612582 1102 12-13 0.04647872300755296 1102 14-15 0.15228855775576022 1102 16-17 0.04692840910757923 1102 18-19 0.1875416064522426 1102 20-21 0.030059303734617515 1102 22-23 0.041445667473595904 1102 24-25 0.02743013844782638 1102 26-27 0.09559765222609684 1102 28-29 0.11391337669604695 1102 30-31 0.16964807739893928 1102 32-33 0.10954760072007019 1102 34-35 0.22249252247469542 1102 36-37 0.2575376063126882 1102 38-39 0.05462264775660941 1102 40-41 0.24575840210768973 1102 42-43 0.08520908745532552 1102 44-45 0.04117861858853189 1102 46-47 -0.16707777617202169 1102 48-49 -0.046510737709134276 1102 50-51 0.04343084072593939 1102 52-53 0.038043842268038475 1102 54-55 0.0758037113822354 1102 56-57 0.03533736361610096 1102 58-59 -0.004942575275208583 1102 60-61 -0.0035765339051856415 1102 62-63 -0.06766298137614513 1102 64-65 0.06721144434186144 1102 66-67 0.04691972296492963 1102 68-69 0.04374219740117269 1102 70-71 -0.17959608926823378 1102 72-73 0.0502164031337351 1102 74-75 -0.03315445973063902 1102 76-77 -0.34670990006079094 1102 78-79 -0.2616983910495705 1102 80-81 -0.48333058624682934 1102 82-83 -0.32048213395080083 1102 84-85 -0.34881274949983165 1102 86-87 -0.26681231805670436 1102 88-89 -0.31103753189973915 1102 90-91 -0.06498493742166644 1102 92-93 -0.19020361695243082 1102 94-95 -0.12579457128193994 1102 96-97 0.13341007413815476 1102 98-99 -0.25227429392637646 1102 100-101 -0.3810178950303005 1102 102-103 -0.436441204810194 1102 104-105 -0.34563966302272675 1102 106-107 -0.2628655985776511 1102 108-109 -0.2853265320652625 1102 110-111 -0.24058307966084058 1102 112-113 -0.23953208992016428 1102 114-115 -0.0699182117295507 1102 116-117 -0.2968810311582608 1102 118-119 -0.24395249793092333 1102 120-121 -0.4146267937653718 1102 122-123 -0.8128465027127874 1102 124-125 -0.885166252931409 1103 1 0.20301000397031288 1103 2 0.2152189481673048 1103 3 0.027655688275821433 1103 4 0.055913295782097805 1103 5 0.013464105253667924 1103 6 -0.2926032636621514 1103 7 0.016704463907814215 1103 8 0.0076923502412356015 1103 9 -0.027013401016688476 1103 10-11 0.03373766918549137 1103 12-13 0.0727499094482269 1103 14-15 0.012718233640377719 1103 16-17 0.07403961933653846 1103 18-19 0.21610984909273867 1103 20-21 0.23205900638106414 1103 22-23 0.1025369550144859 1103 24-25 0.21647266000596233 1103 26-27 0.1927579257913763 1103 28-29 -0.006418172515971321 1103 30-31 -0.22438998385589315 1103 32-33 -0.08705513493262629 1103 34-35 0.0407648983295843 1103 36-37 0.12253165924162346 1103 38-39 0.09400712590112192 1103 40-41 0.035841661102629985 1103 42-43 0.2582168186477176 1103 44-45 0.08237695340864093 1103 46-47 0.061683746278170304 1103 48-49 0.1378112961891702 1103 50-51 0.007793017353947107 1103 52-53 -0.02507688327462887 1103 54-55 0.0524391559257964 1103 56-57 0.12724934696429813 1103 58-59 0.1766661074485505 1103 60-61 0.18384541078705396 1103 62-63 0.31585619792804565 1103 64-65 0.2783473349157539 1103 66-67 0.28771663048797436 1103 68-69 0.13028694911095684 1103 70-71 0.11129002432082657 1103 72-73 0.11528479445101425 1103 74-75 0.31999005409630144 1103 76-77 0.3966962848931175 1103 78-79 0.1253221263456119 1103 80-81 0.006187700996704848 1103 82-83 0.09489104475868615 1103 84-85 -0.003890061423714286 1103 86-87 0.26198637358766064 1103 88-89 0.09427766286683692 1103 90-91 -0.023058086395796096 1103 92-93 0.021006612009802694 1103 94-95 -0.020598317936713073 1103 96-97 0.24273954187529156 1103 98-99 0.1868499998589357 1103 100-101 0.1476321198373789 1103 102-103 0.1052849325671481 1103 104-105 0.14868831794664317 1103 106-107 0.029343738323927226 1103 108-109 0.16234667637957756 1103 110-111 0.1779197451979151 1103 112-113 0.2548404940822131 1103 114-115 0.34981863037571514 1103 116-117 0.08832562956133572 1103 118-119 0.3614742044181689 1103 120-121 0.34644962609784713 1103 122-123 0.10394653921406416 1103 124-125 -0.3572819234636704 1104 1 0.02325332508479505 1104 2 -0.030450901435202127 1104 3 0.040318794881542885 1104 4 -0.07534962075257567 1104 5 -0.04233079903197989 1104 6 -0.0650286306048713 1104 7 0.003890507262113374 1104 8 -0.145445330590249 1104 9 -0.03957770084623036 1104 10-11 0.051326029596410194 1104 12-13 -0.027551036381517235 1104 14-15 -0.13692840014098095 1104 16-17 0.08362048250047138 1104 18-19 -0.047321838466650945 1104 20-21 -0.048890519195559534 1104 22-23 -0.0860704728414845 1104 24-25 0.09425395761799393 1104 26-27 0.060988153122380595 1104 28-29 -0.0773489926876394 1104 30-31 -0.04502212094840985 1104 32-33 0.0011284043110180164 1104 34-35 -0.08068635448000094 1104 36-37 -0.18764543870531014 1104 38-39 -0.07967934737579441 1104 40-41 -0.09571164175898872 1104 42-43 -0.1882563074176602 1104 44-45 -0.16599198053413033 1104 46-47 0.13769217879540463 1104 48-49 -0.013156029692169113 1104 50-51 0.17561783520252305 1104 52-53 0.24378804559957956 1104 54-55 0.1749588041435075 1104 56-57 0.3070754168682086 1104 58-59 0.3659210591011153 1104 60-61 0.563517990775285 1104 62-63 0.4634226096581422 1104 64-65 0.5866829309996895 1104 66-67 0.5306609969932303 1104 68-69 0.4750456644720167 1104 70-71 0.20708432520631348 1104 72-73 0.3786885747530171 1104 74-75 0.6493786588842809 1104 76-77 0.42134065592387415 1104 78-79 0.25591677720319694 1104 80-81 0.394976903150841 1104 82-83 0.5677580251934344 1104 84-85 0.48551875726962734 1104 86-87 0.5012254859860228 1104 88-89 0.7087928308383589 1104 90-91 0.5817378135001157 1104 92-93 0.6285034818714728 1104 94-95 0.4846354242002313 1104 96-97 0.6577287560974376 1104 98-99 0.6781798938113575 1104 100-101 0.47921936395799847 1104 102-103 0.3801140948968751 1104 104-105 0.5563933608634812 1104 106-107 0.5588028655055943 1104 108-109 0.5627260642301195 1104 110-111 0.5795606165077487 1104 112-113 0.8002452247394665 1104 114-115 0.36466906256107734 1104 116-117 0.5079621563900183 1104 118-119 0.6913692882153661 1104 120-121 0.560623872979022 1104 122-123 0.21946108870042025 1104 124-125 0.16700267022174486 1105 1 0.09236097206412097 1105 2 0.029672179691715428 1105 3 0.050149097965849876 1105 4 -0.00513115472239889 1105 5 -0.02336890992677354 1105 6 0.08687587459800739 1105 7 -0.18609073652851293 1105 8 -0.029545173620228127 1105 9 0.18429466188668187 1105 10-11 0.004872111508923638 1105 12-13 0.12421795780746692 1105 14-15 -0.08599779217545489 1105 16-17 -0.029735037512836016 1105 18-19 -0.14148491501961047 1105 20-21 -0.019570390946455518 1105 22-23 0.01286556150479612 1105 24-25 -0.07681885894686502 1105 26-27 -0.03784838272603963 1105 28-29 0.12144974858210844 1105 30-31 0.09662401487149452 1105 32-33 0.29884387424836234 1105 34-35 -0.0687365106332436 1105 36-37 -0.18777649456257706 1105 38-39 -0.07919076519969082 1105 40-41 0.022383793636038263 1105 42-43 0.02501983655518103 1105 44-45 -0.19894974416430955 1105 46-47 -0.1868459328419121 1105 48-49 -0.24340632039113785 1105 50-51 -0.5471088097526788 1105 52-53 -0.30686263002493064 1105 54-55 -0.32818987779840825 1105 56-57 -0.43475865230848854 1105 58-59 -0.34620454067434636 1105 60-61 -0.32925567093279184 1105 62-63 -0.5288101960361367 1105 64-65 -0.5519749085166588 1105 66-67 -0.5523638167440943 1105 68-69 -0.6390612919580398 1105 70-71 -0.6843149206841765 1105 72-73 -0.6849106423274627 1105 74-75 -0.6414907203787443 1105 76-77 -0.3557853076893771 1105 78-79 -0.5673876273131455 1105 80-81 -0.5504912372816335 1105 82-83 -0.5375900933196718 1105 84-85 -0.3621620366986882 1105 86-87 -0.3391726119591105 1105 88-89 -0.5772226552860609 1105 90-91 -0.34068602931007774 1105 92-93 -0.368632966210356 1105 94-95 -0.5664396050924267 1105 96-97 -0.5328899463530483 1105 98-99 -0.7332708982412406 1105 100-101 -0.8366193118506509 1105 102-103 -0.6533779162647768 1105 104-105 -0.4926011102871506 1105 106-107 -0.5233446536404394 1105 108-109 -0.5189050280109271 1105 110-111 -0.44198526343766176 1105 112-113 -0.6735591968549706 1105 114-115 -0.8634847056846411 1105 116-117 -1.0633537632211372 1105 118-119 -0.8576808723930291 1105 120-121 -0.890840165739256 1105 122-123 -0.8153039834656965 1105 124-125 -0.5233713698776334 1106 1 0.06257111410350547 1106 2 0.05206692094205323 1106 3 0.029172440021405066 1106 4 0.13094996772952783 1106 5 0.05004217249535259 1106 6 -0.250125413518397 1106 7 -0.16468085557819734 1106 8 0.05522212786254954 1106 9 0.050044628672921476 1106 10-11 0.12961867605048383 1106 12-13 -0.15283537483270493 1106 14-15 -0.12568462970526895 1106 16-17 0.07103985403700364 1106 18-19 -0.002782071343794712 1106 20-21 -0.04068640229111509 1106 22-23 0.1939058454680378 1106 24-25 0.13888215363970602 1106 26-27 0.06867032384335658 1106 28-29 -0.0428832901595797 1106 30-31 0.03769268938238923 1106 32-33 -0.013904867960846445 1106 34-35 0.02957261997482874 1106 36-37 0.07715525837323156 1106 38-39 -0.08982494591722201 1106 40-41 -0.15171481369640816 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100-101 0.10243603714509675 2313 102-103 0.19119914005117522 2313 104-105 0.12159710726097472 2313 106-107 0.24684377454163453 2313 108-109 0.21638132948633526 2313 110-111 0.12094892218663134 2313 112-113 0.17067379119783865 2313 114-115 0.1280380227873792 2313 116-117 0.1774954471689405 2313 118-119 0.0787192679440345 2313 120-121 0.3778290138736473 2313 122-123 0.3779882185569292 2313 124-125 0.4749713977005108 2314 1 -0.021446486092052908 2314 2 -0.07489004524646248 2314 3 -0.1471767416361658 2314 4 -0.08030515732741605 2314 5 -0.07991760483550792 2314 6 -0.2844841286152331 2314 7 0.020323977808843097 2314 8 -0.22513098717206503 2314 9 -0.19439697623380425 2314 10-11 -0.15190667423119208 2314 12-13 -0.0806554061663931 2314 14-15 -0.1067927531288646 2314 16-17 -0.11481915550754707 2314 18-19 -0.11058188023056204 2314 20-21 -0.055628234978144064 2314 22-23 -0.0939251304316926 2314 24-25 -0.13436810469649174 2314 26-27 -0.18518110783922026 2314 28-29 -0.041645776409424684 2314 30-31 -0.049187442619079036 2314 32-33 -0.01771463251378691 2314 34-35 0.18337432888270655 2314 36-37 -0.21576466295922359 2314 38-39 0.16787930635224768 2314 40-41 -0.014886904497409148 2314 42-43 0.03933094116331404 2314 44-45 -0.02931943873451104 2314 46-47 -0.1737828271321007 2314 48-49 -0.13262059749853705 2314 50-51 -0.3050938509796808 2314 52-53 -0.10641248973264084 2314 54-55 -0.17122174050131633 2314 56-57 -0.10367243423287675 2314 58-59 -0.01708571396545011 2314 60-61 -0.2647032865376566 2314 62-63 -0.1574695583230472 2314 64-65 0.09203125401583279 2314 66-67 -0.10283533081061691 2314 68-69 0.0027981690211973387 2314 70-71 0.05232575317785049 2314 72-73 0.11532705125581444 2314 74-75 0.06522523507199551 2314 76-77 -0.01073799848975554 2314 78-79 -0.09012200724417596 2314 80-81 -0.16765825109282417 2314 82-83 0.17294373462287993 2314 84-85 0.04925442770609223 2314 86-87 -0.045050112977587986 2314 88-89 0.006457822568417981 2314 90-91 0.06036558907194234 2314 92-93 -0.09250245134625956 2314 94-95 -0.11811536637175024 2314 96-97 0.10659831671938136 2314 98-99 -0.01096284620972554 2314 100-101 0.05959953838373977 2314 102-103 -0.16072748219067634 2314 104-105 -0.12273296782592524 2314 106-107 -0.331114092299142 2314 108-109 -0.030433238523958295 2314 110-111 -0.07717438547760835 2314 112-113 -0.15170056787500386 2314 114-115 -0.1962471153840326 2314 116-117 -0.3481944497066003 2314 118-119 -0.3951251427870517 2314 120-121 -0.24043765023251495 2314 122-123 -0.16291829729581764 2314 124-125 0.17980643697230292 2315 1 -0.057514851048168225 2315 2 -0.08284991257739449 2315 3 -0.13846099811125612 2315 4 -0.16242595367376111 2315 5 5.669581394229795E-4 2315 6 -0.10356415882261416 2315 7 -0.09820197370996198 2315 8 -0.23471602289810534 2315 9 -0.1362104152007717 2315 10-11 -0.220171353996939 2315 12-13 -0.033036934848190924 2315 14-15 -0.08782772951897044 2315 16-17 -0.26382393004482196 2315 18-19 -0.1723756235397147 2315 20-21 -0.16682606015704948 2315 22-23 -0.1986568600811296 2315 24-25 -0.18095200072997386 2315 26-27 -0.13706298368465042 2315 28-29 -0.2222182367898924 2315 30-31 -0.0784103908266971 2315 32-33 -0.1576845081157927 2315 34-35 -0.13020191295341021 2315 36-37 -0.22440364321101214 2315 38-39 -0.2313861866892637 2315 40-41 -0.04636468740810784 2315 42-43 -0.11739926151939528 2315 44-45 0.0957521378309707 2315 46-47 -0.11160423011576626 2315 48-49 -0.2806108520206507 2315 50-51 -0.2106663805162512 2315 52-53 -0.36406668709308576 2315 54-55 -0.3253651591917226 2315 56-57 -0.30605257979263456 2315 58-59 -0.041926585774934466 2315 60-61 -0.06235841058986935 2315 62-63 -0.027216460821239963 2315 64-65 0.20050467455962107 2315 66-67 -0.14179130880237878 2315 68-69 -0.14199638203121978 2315 70-71 -0.16350145061964838 2315 72-73 -0.06159120069922608 2315 74-75 -0.04921981487254001 2315 76-77 0.0019652858718366417 2315 78-79 0.10232593895791098 2315 80-81 0.015106803499005395 2315 82-83 -0.01421722758745858 2315 84-85 0.08649222234566523 2315 86-87 0.007283421369479015 2315 88-89 -0.07291816809274465 2315 90-91 -0.07399261463980267 2315 92-93 -0.20821720732203985 2315 94-95 -0.2206925376658866 2315 96-97 -0.18507134671170533 2315 98-99 -0.05787661684936296 2315 100-101 -0.06471650776827076 2315 102-103 -0.24173728575968667 2315 104-105 -0.22629561357241101 2315 106-107 -0.2771972289986273 2315 108-109 -0.07172945900467553 2315 110-111 -0.13882324675323332 2315 112-113 -0.45266239094049965 2315 114-115 -0.4945851559427048 2315 116-117 -0.2804332263628204 2315 118-119 -0.5709282525986445 2315 120-121 -0.389380530058574 2315 122-123 -0.23394403443070644 2315 124-125 0.23365034058529588 2316 1 -0.15035591719392016 2316 2 -0.3403678559932217 2316 3 0.003993798181632258 2316 4 0.052384435463366685 2316 5 0.03361052728839553 2316 6 -0.0020068617158059965 2316 7 -0.3847485962234316 2316 8 -0.2717215152111976 2316 9 -0.16356534066223816 2316 10-11 -0.19817385262157217 2316 12-13 -0.23212710497076472 2316 14-15 -0.19276254846756302 2316 16-17 -0.15704939454676747 2316 18-19 -0.30378435205899024 2316 20-21 -0.28455520727011674 2316 22-23 -0.26852795280213115 2316 24-25 -0.26814856226090455 2316 26-27 -0.41157447008381354 2316 28-29 -0.22787368789474272 2316 30-31 -0.10684054409873767 2316 32-33 -0.23713660450987106 2316 34-35 -0.11366459142534069 2316 36-37 -0.10546932285738819 2316 38-39 -0.22905211252573565 2316 40-41 -0.17536765990981706 2316 42-43 0.061857281127075225 2316 44-45 0.016559579184274753 2316 46-47 0.027775661817649677 2316 48-49 0.05047032926805173 2316 50-51 0.012035015479028743 2316 52-53 0.21757188240547265 2316 54-55 0.2707753909153112 2316 56-57 0.3993063979888163 2316 58-59 0.6289852003461824 2316 60-61 0.5284561424508176 2316 62-63 0.46080843220330436 2316 64-65 0.34419818978830463 2316 66-67 0.28982702734525745 2316 68-69 0.20085969139924842 2316 70-71 0.28678665393557523 2316 72-73 0.3029237391409829 2316 74-75 0.32117303699885014 2316 76-77 0.25061798481722874 2316 78-79 0.21303026437516337 2316 80-81 0.24851951654065374 2316 82-83 0.31642468164989523 2316 84-85 0.19643135279886792 2316 86-87 0.18220868509161292 2316 88-89 0.3783085097040306 2316 90-91 0.27336952844282436 2316 92-93 0.26123361080449925 2316 94-95 0.10216182669968887 2316 96-97 0.1706690985915138 2316 98-99 -0.055092324144457194 2316 100-101 -0.05846359017556679 2316 102-103 -0.1861217010652787 2316 104-105 -0.14194937679912556 2316 106-107 -0.28139573759233016 2316 108-109 -0.20555877426994584 2316 110-111 -0.1964865313075954 2316 112-113 -0.2595260520541167 2316 114-115 -0.32222949247594457 2316 116-117 -0.3060596657379797 2316 118-119 -0.1777059661099294 2316 120-121 -0.18361264790777554 2316 122-123 -0.18145031806909628 2316 124-125 -0.1653552237627629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 19.0 15 234.0 16 433.0 17 480.0 18 806.0 19 2504.0 20 8911.0 21 24898.0 22 48099.0 23 73672.0 24 82739.0 25 56701.0 26 32938.0 27 23789.0 28 16650.0 29 15404.0 30 17411.0 31 20243.0 32 25412.0 33 33890.0 34 47498.0 35 69017.0 36 119283.0 37 420421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.20051055339464 16.7967157671056 11.892545341857751 20.110228337642006 2 15.510858100033992 23.429368909073705 39.651075997939465 21.408696992952837 3 20.081790561495357 33.468073996979285 26.62240724971352 19.827728191811833 4 12.707984884818144 16.718957672759643 35.642198097197316 34.9308593452249 5 13.177426646061333 39.655193560482616 30.207052070520707 16.960327722935347 6 27.644351229837085 34.82800853649562 21.16146802493666 16.366172208730635 7 27.030401152231793 33.951501484532216 21.962578749418498 17.055518613817494 8 29.174157126186646 28.067408879217 22.40865143694172 20.349782557654635 9 32.37569341505381 13.42448039865014 21.171104873441895 33.02872131285415 10-11 24.63323030666204 26.48109600754127 27.008275424634586 21.8773982611621 12-13 27.015371649442987 24.37180888902906 29.90204581532995 18.710773646198 14-15 24.801086686080538 23.085946671432524 31.274858688757828 20.83810795372911 16-17 19.826852114674992 26.71185472538486 30.388881880271796 23.072411279668355 18-19 20.10535703647635 24.215560531673695 36.41401478117346 19.265067650676507 20-21 22.98681854339911 23.622456310033186 35.01802966747616 18.372695479091544 22-23 21.182274856936605 25.299092734517085 34.15049428272061 19.368138125825705 24-25 19.791710927182052 26.41341986127203 34.730483576702284 19.06438563484363 26-27 20.137784263277815 26.58055223224788 34.7655560369545 18.51610746751981 28-29 19.801719088754165 27.656292643735263 34.50189488772391 18.04009337978667 30-31 20.436223809246375 27.711395428196457 34.086758520933984 17.765622241623184 32-33 18.630642375370872 28.03565632100677 34.48161966503314 18.85208163858922 34-35 18.279643846718287 29.12286850146207 33.71334698660592 18.884140665213724 36-37 18.682802293307915 29.92514508396647 32.71306141187026 18.678991210855365 38-39 18.503600853918662 29.488012413625725 32.829825645860325 19.17856108659529 40-41 18.282790673225605 30.140786459268732 32.61782214648976 18.958600721015905 42-43 18.44407279175223 30.059012762736455 32.21679831231909 19.280116133192227 44-45 18.268924142232876 29.85009444111535 31.90011494132035 19.98086647533142 46-47 17.822826973038964 29.26872858796778 32.107152040376945 20.801292398616315 48-49 18.073227551053485 29.285062830679138 31.65539543070314 20.986314187564233 50-51 17.266301771700284 28.397365186146374 32.168175282761474 22.168157759391868 52-53 17.743112722569837 27.25229152409901 32.417716738618616 22.586879014712544 54-55 18.032151213228527 26.537544800511753 32.512859608997786 22.917444377261933 56-57 18.35191026989134 25.600727304591658 32.62999474237645 23.41736768314055 58-59 18.12195781325788 25.394331372867757 33.167870214053394 23.31584059982097 60-61 18.50419765267946 24.944410293439166 33.257344303911715 23.29404774996966 62-63 18.245837854983293 24.653839127251825 33.56186559078779 23.53845742697709 64-65 17.927791175742602 24.507498844562434 33.91716334053976 23.647546639155205 66-67 17.824665426141443 23.95873851900912 34.19762723268258 24.018968822166855 68-69 17.991864747707307 23.933244674327085 34.24484779035824 23.830042787607365 70-71 17.693166247901797 23.252883169857338 34.25027946860666 24.803671113634213 72-73 18.19147892333624 23.291342956164605 33.78210384667949 24.73507427381966 74-75 18.22757330137404 23.045822338565266 33.35335169591012 25.373252664150574 76-77 18.10461587521858 23.014677796350615 32.47122962682618 26.40947670160462 78-79 18.517116795099575 22.65592420881474 31.820786156579516 27.00617283950617 80-81 18.42109874090194 22.67300771298311 30.875323710502062 28.030569835612884 82-83 18.739870304858925 22.552096200783918 29.612132330394953 29.0959011639622 84-85 19.178720662259025 22.379627597571986 28.35968851257615 30.08196322759284 86-87 19.307299346171604 21.877099886020268 27.790641747070126 31.024959020738002 88-89 19.64629613728641 21.94170444449507 26.857451271420334 31.55454814679819 90-91 20.90886866337844 21.49253051278299 26.125461448613486 31.473139375225085 92-93 21.16442035290829 21.91840540201875 25.113805344009787 31.803368901063177 94-95 21.300873707413828 21.931059441985713 25.276086898419734 31.49197995218072 96-97 21.652346129073248 21.520635157102358 25.30559486826712 31.521423845557273 98-99 22.57598007861307 21.574653504905868 25.163543581538722 30.68582283494234 100-101 22.019994199246256 21.38340090656493 25.817341099216545 30.779263794972273 102-103 22.512752950495813 21.90519419579191 26.22465785114263 29.357395002569646 104-105 21.876919383484168 21.976190663943495 27.021020452687555 29.125869499884782 106-107 21.678361502277493 22.089046046608203 27.922255992051774 28.310336459062523 108-109 21.39757349407829 22.156004434766572 28.828085336403152 27.618336734751985 110-111 21.130712887395234 22.46777373652065 29.488186441205112 26.913326934879006 112-113 20.342739047607367 22.876837079805842 30.094357929355738 26.686065943231053 114-115 19.91663220751186 22.893815574603487 30.907496443114358 26.282055774770292 116-117 19.625689648752772 23.419913135467933 31.129611256031346 25.82478595974795 118-119 19.313733735627956 24.11941106590553 31.200742562981187 25.366112635485326 120-121 18.985117201599365 24.73853477851018 31.60049656052116 24.675851459369294 122-123 18.551327607293167 25.720091602625427 31.351690653658675 24.376890136422734 124-125 17.886351683029453 26.878462482468446 31.903269635343616 23.331916199158485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 15.5 2 70.5 3 145.5 4 160.0 5 208.0 6 380.5 7 705.5 8 1208.5 9 1530.0 10 1653.5 11 1839.5 12 2139.5 13 2484.0 14 2852.5 15 3327.5 16 3811.5 17 4368.0 18 5018.5 19 5644.5 20 6349.0 21 7259.5 22 8268.5 23 9246.5 24 10206.0 25 11490.0 26 13189.0 27 14799.5 28 16544.5 29 19108.0 30 21760.5 31 23891.5 32 26097.5 33 27911.0 34 28784.5 35 28690.5 36 28064.0 37 27369.5 38 25738.5 39 23315.0 40 21406.5 41 20104.0 42 19326.0 43 20427.5 44 22350.5 45 23767.5 46 26997.0 47 29411.0 48 31010.0 49 34454.0 50 38690.5 51 42167.5 52 46679.0 53 52514.0 54 51798.0 55 44971.5 56 38680.0 57 32465.5 58 26551.0 59 21716.5 60 17406.0 61 14050.5 62 11392.5 63 8528.5 64 5930.0 65 4572.5 66 3448.5 67 2590.5 68 2100.5 69 1761.5 70 1540.5 71 1331.5 72 1155.5 73 1179.5 74 970.0 75 715.5 76 555.5 77 384.5 78 263.0 79 141.0 80 94.0 81 78.0 82 59.0 83 36.5 84 14.0 85 6.0 86 4.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06386602327561737 2 0.0 3 0.0 4 0.007271440235769879 5 0.0 6 0.0 7 0.0013141157052596166 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.322732799977572E-4 26-27 0.008979790652607381 28-29 0.008016105802083662 30-31 0.003723327831568914 32-33 0.004862228109460582 34-35 0.007840890374715713 36-37 0.003854739402094876 38-39 0.010994768067338793 40-41 6.132539957878211E-4 42-43 3.5043085473589777E-4 44-45 0.0 46-47 7.446655663137828E-4 48-49 0.004467993397882696 50-51 0.010425317928392958 52-53 0.015856996176799375 54-55 0.024705375258880797 56-57 0.022077143848361562 58-59 0.02728980281255804 60-61 0.024398748260986883 62-63 0.016076015461009313 64-65 0.009593044648395202 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.256462821038466E-4 84-85 0.002233996698941348 86-87 0.0018397619873634634 88-89 0.011389002778916678 90-91 0.01831001215995066 92-93 0.021814320707309636 94-95 0.024573963688354832 96-97 0.027771645237819897 98-99 0.014542880471539758 100-101 0.019361304724158354 102-103 0.021507693709415725 104-105 0.01182704134733655 106-107 0.005694501389458339 108-109 0.0014455272757855785 110-111 0.0026720352673612205 112-113 4.380385684198722E-5 114-115 3.5043085473589777E-4 116-117 0.0017083504168375016 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.057120229321951335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1141452.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.24600351866495 #Duplication Level Percentage of deduplicated Percentage of total 1 69.0407342202054 28.47654366577803 2 17.245909048107947 14.226496505616721 3 6.252445979694842 7.73665226636268 4 2.438475608354826 4.023094940895272 5 1.2748817289299748 2.62918881386637 6 0.7678463057428281 1.900235485707754 7 0.5001163617941005 1.4439460852810966 8 0.35340397559997494 1.1661201296885397 9 0.2685464319884026 0.9968820370846714 >10 1.560518183132303 12.231131190273786 >50 0.15790648365805193 4.521293047392579 >100 0.12032818088996489 9.387879652799658 >500 0.012945359617884847 3.571737563017695 >1k 0.005305475253231494 4.045459991422344 >5k 2.1221901012925974E-4 0.764065609302327 >10k+ 4.244380202585195E-4 2.8792730155104533 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21386 1.8735785648454775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11508 1.0081895690751779 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8729 0.7647277327474129 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4625 0.4051856757883819 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4113 0.36033052638218693 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2854 0.2500324148540631 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2305 0.20193578004156107 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 2137 0.18721768414265338 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.1806471056163553 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2033 0.17810648191952005 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 1934 0.1694333182648066 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 1900 0.16645465599955145 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 1884 0.16505293258060785 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 1747 0.15305067580590337 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 1697 0.14867029012170463 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1683 0.147443782130129 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 1588 0.1391210493301514 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 1557 0.1364052102059482 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1534 0.1343902327912168 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 1339 0.11730672862284178 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA 1238 0.10845834954076036 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 1223 0.10714423383550076 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 1188 0.10407796385656164 No Hit GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1168 0.10232580958288216 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7521542736794889E-4 0.0 12-13 0.0 0.0 0.0 3.94234711577885E-4 0.0 14-15 0.0 0.0 0.0 6.570578526298083E-4 0.0 16-17 0.0 0.0 0.0 0.0014017234189435913 0.0 18-19 0.0 0.0 0.0 0.002409212126309297 0.0 20-21 0.0 0.0 0.0 0.004205170256830774 0.0 22-23 0.0 0.0 0.0 0.015637976892589438 8.760771368397444E-5 24-25 0.0 0.0 0.0 0.04406667998303915 1.7521542736794889E-4 26-27 0.0 0.0 0.0 0.09006072966712572 1.7521542736794889E-4 28-29 0.0 0.0 0.0 0.1834067485974005 2.1901928420993611E-4 30-31 0.0 0.0 0.0 0.35599394455483013 2.628231410519233E-4 32-33 0.0 0.0 0.0 0.6800110736150096 2.628231410519233E-4 34-35 0.0 0.0 0.0 0.9388480636943122 2.628231410519233E-4 36-37 0.0 0.0 0.0 1.2593170803502907 2.628231410519233E-4 38-39 0.0 0.0 0.0 1.703575796441725 2.628231410519233E-4 40-41 0.0 0.0 0.0 2.319983669922169 2.628231410519233E-4 42-43 0.0 0.0 0.0 2.8677070958743776 2.628231410519233E-4 44-45 0.0 0.0 0.0 3.3548935916709595 2.628231410519233E-4 46-47 0.0 0.0 0.0 3.8180317700612907 2.628231410519233E-4 48-49 0.0 0.0 0.0 4.278191286186367 2.628231410519233E-4 50-51 0.0 0.0 0.0 4.742818795709324 2.628231410519233E-4 52-53 0.0 0.0 0.0 5.256156194040573 2.628231410519233E-4 54-55 0.0 0.0 0.0 5.746496567529778 2.628231410519233E-4 56-57 0.0 0.0 0.0 6.214234150888517 2.628231410519233E-4 58-59 0.0 0.0 0.0 6.723935829101881 2.628231410519233E-4 60-61 0.0 0.0 0.0 7.238762558565757 2.628231410519233E-4 62-63 0.0 0.0 0.0 7.774220904602208 2.628231410519233E-4 64-65 0.0 0.0 0.0 8.291062611480815 3.0662699789391057E-4 66-67 0.0 0.0 0.0 8.791171245045783 3.5043085473589777E-4 68-69 0.0 0.0 0.0 9.298770338130732 3.5043085473589777E-4 70-71 0.0 0.0 0.0 9.838477658280855 3.5043085473589777E-4 72-73 0.0 0.0 0.0 10.377265097437299 4.3803856841987223E-4 74-75 0.0 0.0 0.0 10.882411174539095 4.3803856841987223E-4 76-77 0.0 0.0 0.0 11.40669953708084 4.3803856841987223E-4 78-79 0.0 0.0 0.0 11.943822429677288 4.3803856841987223E-4 80-81 0.0 8.760771368397444E-5 0.0 12.49649569145264 4.3803856841987223E-4 82-83 0.0 8.760771368397444E-5 0.0 13.11211509550993 4.3803856841987223E-4 84-85 0.0 8.760771368397444E-5 0.0 13.653005119794788 4.3803856841987223E-4 86-87 0.0 8.760771368397444E-5 0.0 14.17194941180181 4.3803856841987223E-4 88-89 0.0 8.760771368397444E-5 0.0 14.677927762183604 4.3803856841987223E-4 90-91 0.0 8.760771368397444E-5 0.0 15.214305989213738 4.3803856841987223E-4 92-93 0.0 8.760771368397444E-5 0.0 15.705697655267151 5.256462821038466E-4 94-95 0.0 8.760771368397444E-5 0.0 16.202652411139496 5.256462821038466E-4 96-97 0.0 8.760771368397444E-5 0.0 16.702804848561307 5.256462821038466E-4 98-99 0.0 8.760771368397444E-5 0.0 17.21850765516202 5.256462821038466E-4 100-101 0.0 8.760771368397444E-5 0.0 17.733903834764842 5.256462821038466E-4 102-103 0.0 8.760771368397444E-5 0.0 18.241152496995056 5.256462821038466E-4 104-105 0.0 8.760771368397444E-5 0.0 18.749408647932633 5.256462821038466E-4 106-107 0.0 8.760771368397444E-5 0.0 19.256044056167056 5.256462821038466E-4 108-109 0.0 8.760771368397444E-5 0.0 19.790494913496143 5.256462821038466E-4 110-111 0.0 8.760771368397444E-5 0.0 20.316885861166305 5.256462821038466E-4 112-113 0.0 8.760771368397444E-5 0.0 20.81738872944285 5.256462821038466E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 350 0.0 64.63862 1 GTATAAC 380 0.0 59.535576 1 TACAACG 290 0.0 57.43982 2 AGATTAC 675 0.0 55.525154 2 TAACGCA 505 0.0 48.299816 4 ATAACGC 520 0.0 48.050617 3 ATAGTAC 505 0.0 47.121773 4 TCAAACG 195 0.0 45.762493 3 GATTACT 835 0.0 44.173138 3 GTACAAC 430 0.0 42.920998 1 TATCACG 225 0.0 42.304882 2 AACGCAG 18525 0.0 42.195988 6 ACAACGC 385 0.0 41.72113 3 CATAGTA 610 0.0 39.985916 3 ACGGTAC 300 0.0 39.660828 3 TAGTACT 600 0.0 39.65909 5 GTACATG 18205 0.0 38.360466 1 TACATGG 18245 0.0 38.247864 2 AAACGCA 520 0.0 37.7524 5 ACATGGG 18470 0.0 37.65309 3 >>END_MODULE