FastQCFastQC Report
Fri 10 Feb 2017
SRR3557352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753055
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612032.2230939810501424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326461.185809945678528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243160.8832369858212058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118420.43014033500965293No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA83570.30355368853873244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62940.22861875262208708No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60110.21833926310952742No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA52700.19142370929748953No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA52310.1900071012021191No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46220.1678862209436426No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45440.16505300475290177No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.16425389249397487No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43280.15720717530161948No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA43170.15680761917215602No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC40870.14845326373792023No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC39370.14300477106341863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC33950.12331755086621952No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA31900.11587127754440066No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA27710.10065182134029287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7000.049.3343471
TCACGCA3850.046.3571744
AACGCAG486950.042.8091056
AGTGTAC9750.040.881483
ATAGTAC8850.040.3333634
GTATCAA519750.039.2131581
CGCAGAG550300.037.956648
ACGCAGA552050.037.728557
GTACATG437200.036.7844161
TACATGG440100.036.59262
ACATGGG435250.036.330613
GCAGAGT576450.036.2657439
CATGGGT36700.034.8520934
CATGGGG100900.034.610144
TATCAAC588550.034.4587972
CATGGGC114500.034.084334
TACAACG5600.033.9952622
ATCAACG596900.033.946853
AGATTAC11400.033.920712
GCATAGT10700.033.9158332
Produced by FastQC (version 0.11.5)