FastQCFastQC Report
Fri 10 Feb 2017
SRR3557345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195692
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407991.2766874905341314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222850.6973450507746054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159170.49807678587298154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83040.2598498228239768No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58480.18299635884809926No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54040.16910265444855135No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT50280.15733681468677205No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45130.14122136926837756No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC43570.1363397974523202No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40640.12717120423369963No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC40270.12601339553373728No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA36770.11506115107463423No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36620.1145917691692441No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.1042966593776872No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT32660.10220008686694462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7600.065.8051451
TACAACG5850.041.6967932
AGATTAC15700.040.5470052
TATCACG5200.040.0441782
AACGCAG386750.040.024996
TAACGCA8150.039.419474
TAGTACT13850.038.2291375
ATAGTAC13850.037.8013734
GTATCAA407200.036.6702541
GCATAGT13800.036.6449782
TCACGCA5950.035.9964944
ACGCAGA437850.035.3538137
ACAACGC7100.035.1937523
CGCAGAG441700.035.1668748
CATGGGG107600.034.9445534
GTACATG397000.034.538031
ACATGGG388200.034.3907783
TACATGG399550.034.2030262
GATTACT19000.033.504633
GCAGAGT465300.033.408789