FastQCFastQC Report
Fri 10 Feb 2017
SRR3557339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304848
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451651.0491659635833832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241080.5600197730558664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180590.4195037780660316No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87890.20416516448432095No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC78260.18179503666563837No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT74070.17206182425024066No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC70760.1643728187383155No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA62790.14585880848754706No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50900.11823878566676455No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT50490.11728637108673755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49000.11382515712517607No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT47840.11113052075241682No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47720.11085176526557965No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA46940.10903985460113806No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45430.1055321813917704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9750.054.952481
AACGCAG473550.037.7359666
ATAGTAC16000.034.949374
GTATCAA490350.034.855911
GTACTGG34950.033.5558661
GTACATG491250.033.1924171
ACATGGG479450.033.1655433
ACGCAGA538300.033.1637047
CGCAGAG540900.033.0812768
TACATGG496300.032.8665922
CATGGGG138300.032.5185434
TAGTACT17650.032.0184485
TACTGGT34150.031.878062
GCATAGT17550.031.8626732
ACGGTAC12150.031.3353963
TACAACG7800.031.2694872
GCAGAGT574150.031.1862059
AGATTAC19500.031.1169532
CATGGGT50550.030.9503864
TATCAAC552300.030.8374042
Produced by FastQC (version 0.11.5)