FastQCFastQC Report
Fri 10 Feb 2017
SRR3557331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644469
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199841.2152250969765923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110260.6704899879535583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70540.4289530541469617No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT47080.28629302224608677No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45190.27479995062235896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44160.26853653063694116No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT40110.24390852001466737No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG36830.2239628719057641No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35800.21769945192034634No Hit
CTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCT30490.18540939354892066No Hit
GGCTACTATAGTAACTACTATGCTATGGACTACTGGGGTCAAGGAACCTC30410.18492291432675229No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG29420.17890273395241868No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28730.1747068506612165No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG25250.15354500449689232No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24880.1512950380943636No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24700.15020045984448477No Hit
GCATAGTAGTTACTATAGTAGCCTGTGCAGTAATAAATTCCAGTGTCTTC23870.14515323791448792No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC23870.14515323791448792No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG21890.1331128771658207No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA21660.13171424940208665No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC21170.12873456416630535No Hit
CTCTGAAGCCACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAG20560.12502516009727152No Hit
CTATAGTAGCCTGTGCAGTAATAAATTCCAGTGTCTTCAGCCCTTAAGTT20390.12399139175016372No Hit
CTACTATAGTAACTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAG20330.12362653233353746No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC20040.1218630451531771No Hit
CATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGGGCACATGCAGA19710.11985631836173256No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19290.11730230244534862No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT18540.11274155973752013No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.11219427061258072No Hit
ATAGTAGCCTGTGCAGTAATAAATTCCAGTGTCTTCAGCCCTTAAGTTGT17930.10903215566848629No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA17720.10775514771029432No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17420.10593085062716294No Hit
GCCCTGCACCACGCAAGTCTAAACTCATCTGCGAGGCCACGAACTTCACT17320.10532275159945248No Hit
GTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAG17190.10453222286342886No Hit
CTCCTGGGCTTCTCAAAGTGGTGAACCACTGGAAACCAAAATTAAAATCA17160.10434979315511572No Hit
GTACAATGTCTCCCTGATCATGTCTGACACAGGCGGCACCTGCTATTGAC16730.10173496733596073No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16540.10057957918331085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3500.059.46561
TACAACG2450.058.1846732
AGATTAC5850.056.858532
GATTACT6650.050.001673
GTACAAC3050.048.7422941
TAGTACT4550.046.979595
ATAGTAC4450.046.7009964
TAACGCA3500.044.1086164
AGTGTAC3650.043.9227033
ACAACGC3450.041.305733
TGAGTAC2250.039.5846565
TATCACG1850.038.5276872
CTGAGTA2550.037.2561454
ATTACTT9000.036.945684
TAAGGTA2850.035.417855
CAGATTA10050.034.9101521
TCACGCA2051.8189894E-1234.757264
GTATAAC5850.034.5612031
ATCACGC2101.8189894E-1233.9297073
TACTGGT4600.033.5721362
Produced by FastQC (version 0.11.5)