##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557330.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3417733 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.382624096147943 33.0 32.0 33.0 27.0 34.0 2 31.74163019756078 33.0 32.0 34.0 27.0 34.0 3 31.924799567432565 33.0 32.0 34.0 27.0 34.0 4 31.812841728713156 33.0 32.0 34.0 27.0 34.0 5 31.955756052330596 33.0 32.0 34.0 28.0 34.0 6 35.31278452705346 38.0 36.0 38.0 29.0 38.0 7 35.63280191869874 38.0 36.0 38.0 30.0 38.0 8 35.842109374839985 38.0 37.0 38.0 31.0 38.0 9 35.95543449415153 38.0 37.0 38.0 31.0 38.0 10-11 35.958932426845514 38.0 37.0 38.0 31.0 38.0 12-13 35.91718618745233 38.0 37.0 38.0 31.0 38.0 14-15 35.89409632057273 38.0 37.0 38.0 31.0 38.0 16-17 35.86099279844271 38.0 37.0 38.0 31.0 38.0 18-19 35.83532417541101 38.0 37.0 38.0 31.0 38.0 20-21 35.81682799680373 38.0 37.0 38.0 31.0 38.0 22-23 35.72129010662916 38.0 37.0 38.0 29.0 38.0 24-25 35.656376170988196 38.0 37.0 38.0 29.0 38.0 26-27 35.42766842816569 38.0 36.0 38.0 29.0 38.0 28-29 35.4336612895156 38.0 36.0 38.0 29.0 38.0 30-31 35.370161302828514 38.0 36.0 38.0 28.0 38.0 32-33 35.323268230724864 38.0 36.0 38.0 28.0 38.0 34-35 35.122456025675504 38.0 36.0 38.0 27.0 38.0 36-37 34.977492829311124 38.0 36.0 38.0 26.5 38.0 38-39 34.79688334343262 38.0 36.0 38.0 25.0 38.0 40-41 34.5508789013068 38.0 35.0 38.0 24.5 38.0 42-43 34.09640264467704 38.0 34.5 38.0 16.0 38.0 44-45 33.80011062888762 38.0 34.0 38.0 16.0 38.0 46-47 33.34880401716576 38.0 33.5 38.0 15.0 38.0 48-49 32.93796121581177 38.0 32.5 38.0 15.0 38.0 50-51 32.586943157935394 38.0 30.0 38.0 15.0 38.0 52-53 32.331275585307566 38.0 29.0 38.0 15.0 38.0 54-55 32.145666440298285 38.0 28.0 38.0 15.0 38.0 56-57 31.927458347389923 38.0 26.5 38.0 15.0 38.0 58-59 31.814501162027575 38.0 26.0 38.0 15.0 38.0 60-61 31.69903705175331 38.0 26.0 38.0 15.0 38.0 62-63 31.593441617586862 38.0 26.0 38.0 15.0 38.0 64-65 31.411766513065825 38.0 25.0 38.0 15.0 38.0 66-67 31.3092089112871 38.0 25.0 38.0 15.0 38.0 68-69 31.16489746273334 38.0 25.0 38.0 15.0 38.0 70-71 31.0272158474638 38.0 24.0 38.0 15.0 38.0 72-73 30.830848401557407 37.5 24.0 38.0 14.0 38.0 74-75 30.722783201613467 37.0 24.0 38.0 14.0 38.0 76-77 30.62920479744907 37.0 24.0 38.0 14.0 38.0 78-79 30.47462235932415 37.0 22.5 38.0 14.0 38.0 80-81 30.30398410291266 37.0 21.0 38.0 14.0 38.0 82-83 30.195082676148196 37.0 21.0 38.0 14.0 38.0 84-85 30.104134377963405 37.0 18.5 38.0 14.0 38.0 86-87 30.0667939830291 37.0 16.0 38.0 13.5 38.0 88-89 29.958036950224024 37.0 16.0 38.0 13.5 38.0 90-91 29.864640391745056 37.0 15.0 38.0 13.0 38.0 92-93 29.796896363759252 37.0 15.0 38.0 13.0 38.0 94-95 29.764374367453513 37.0 15.0 38.0 13.0 38.0 96-97 29.71070867736011 36.5 15.0 38.0 13.0 38.0 98-99 29.620525652530496 36.0 15.0 38.0 13.0 38.0 100-101 29.53206057933724 36.0 15.0 38.0 13.0 38.0 102-103 29.40033876256571 36.0 15.0 38.0 13.0 38.0 104-105 29.327307896784212 36.0 15.0 38.0 13.0 38.0 106-107 29.160642888136667 35.0 15.0 38.0 13.0 38.0 108-109 29.038390945108937 35.0 15.0 38.0 13.0 38.0 110-111 28.853610712129942 35.0 15.0 38.0 13.0 38.0 112-113 28.69075758697359 35.0 14.0 38.0 13.0 38.0 114-115 28.54967517942449 34.5 14.0 38.0 13.0 38.0 116-117 28.391374926010897 34.0 14.0 38.0 13.0 38.0 118-119 28.292545965410405 34.0 14.0 38.0 13.0 38.0 120-121 28.067356197807143 34.0 14.0 38.0 13.0 38.0 122-123 28.00027474352151 34.0 14.0 38.0 13.0 38.0 124-125 26.788918268337518 29.5 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5217827498158982 1101 2 -0.36448634915164035 1101 3 -0.5261117716525128 1101 4 -0.4265723662020413 1101 5 -0.553080607319135 1101 6 0.04373687678850757 1101 7 -0.006380791649007733 1101 8 -0.0016207077702858896 1101 9 -0.10545387696777198 1101 10-11 -0.11463909302457154 1101 12-13 0.01878092147352106 1101 14-15 -0.0968481032636177 1101 16-17 0.06606295291335584 1101 18-19 -0.20182267068069137 1101 20-21 -0.043428426971161116 1101 22-23 0.013592290272704588 1101 24-25 -0.1336468324944633 1101 26-27 -0.014715367017934966 1101 28-29 -0.10910906592856406 1101 30-31 -0.1436108898425985 1101 32-33 -0.10545255762706773 1101 34-35 -0.13439533083320754 1101 36-37 -0.28304352958754464 1101 38-39 -0.24416263871182053 1101 40-41 -0.22139723615891427 1101 42-43 -0.3267858826866501 1101 44-45 -0.3029039945933576 1101 46-47 -0.3035675660981596 1101 48-49 -0.4469561501299566 1101 50-51 -0.5721800386840101 1101 52-53 -0.44828223383787247 1101 54-55 -0.5888569222443039 1101 56-57 -0.44410542076094117 1101 58-59 -0.4837294992967429 1101 60-61 -0.5160235767462318 1101 62-63 -0.5160632889560404 1101 64-65 -0.4950999289986271 1101 66-67 -0.3902397275436513 1101 68-69 -0.5007796787734655 1101 70-71 -0.37495177418935555 1101 72-73 -0.5002918152739717 1101 74-75 -0.6890445223348678 1101 76-77 -0.650615224664147 1101 78-79 -0.6735602500619926 1101 80-81 -0.609712023171209 1101 82-83 -0.5338332455746233 1101 84-85 -0.4840644229988804 1101 86-87 -0.49554372044210027 1101 88-89 -0.6624263349942261 1101 90-91 -0.7158361655779686 1101 92-93 -0.6110594121831063 1101 94-95 -0.6568719010322894 1101 96-97 -0.6814632719015954 1101 98-99 -0.6356475023417296 1101 100-101 -0.7020241144484949 1101 102-103 -0.657716065320006 1101 104-105 -0.7360069806893783 1101 106-107 -0.6262098610064974 1101 108-109 -0.951623051494142 1101 110-111 -0.8893512273493407 1101 112-113 -0.8467280733467213 1101 114-115 -0.8386269720800357 1101 116-117 -0.9263574620548738 1101 118-119 -1.0355356706744594 1101 120-121 -1.0987848186988565 1101 122-123 -0.7864496360563642 1101 124-125 -0.9084587021216919 1102 1 -0.4505832785717736 1102 2 -0.623879453371778 1102 3 -0.44020419556641954 1102 4 -0.3490999547771203 1102 5 -0.4990234044755084 1102 6 -0.08621013813735345 1102 7 0.012970303317523246 1102 8 -0.025648949008505895 1102 9 -0.05183602511242924 1102 10-11 -0.10356234809228937 1102 12-13 0.04403784822317647 1102 14-15 -0.06791461819589273 1102 16-17 0.0015160697647189636 1102 18-19 -0.009820908036346054 1102 20-21 -0.04507274132487282 1102 22-23 0.005687060573556835 1102 24-25 -0.07442901013506287 1102 26-27 0.20222286285331137 1102 28-29 0.006487173601286145 1102 30-31 -0.04390338811396788 1102 32-33 -0.05798763154518127 1102 34-35 -0.10125282586630391 1102 36-37 -0.015196170695247702 1102 38-39 0.021514214004163534 1102 40-41 0.05480022455145672 1102 42-43 0.01575834975781021 1102 44-45 0.07085240582411956 1102 46-47 0.05846630426027133 1102 48-49 -0.05372606693025972 1102 50-51 0.04791283421947412 1102 52-53 -0.09726325303297045 1102 54-55 -0.07614115014272471 1102 56-57 -0.06531669693098507 1102 58-59 -0.17600474506425812 1102 60-61 -0.18273949688897417 1102 62-63 -0.09293637697212631 1102 64-65 -0.132394211905293 1102 66-67 -0.09422388770060763 1102 68-69 -0.16025117369070685 1102 70-71 -0.10545135135497219 1102 72-73 -0.15330817269962083 1102 74-75 -0.2739154827505139 1102 76-77 -0.2993601453409376 1102 78-79 -0.28285051620575175 1102 80-81 -0.3231004935719106 1102 82-83 -0.34636606237967626 1102 84-85 -0.3101280805121043 1102 86-87 -0.37334720319001846 1102 88-89 -0.41737020334351627 1102 90-91 -0.30501168952624624 1102 92-93 -0.2577916935882065 1102 94-95 -0.4632878197807102 1102 96-97 -0.5203374965219609 1102 98-99 -0.428612730354601 1102 100-101 -0.47741474145896845 1102 102-103 -0.487171053928229 1102 104-105 -0.5942057239864695 1102 106-107 -0.6633782527769441 1102 108-109 -0.578370056538116 1102 110-111 -0.8640688004624444 1102 112-113 -0.7414869950493248 1102 114-115 -0.6722733878322167 1102 116-117 -0.6530821299144165 1102 118-119 -0.6773205043532009 1102 120-121 -1.0543222647379942 1102 122-123 -1.048622816705791 1102 124-125 -1.0898372479625635 1103 1 -0.49378313715589783 1103 2 -0.30999888250656227 1103 3 -0.2612299823051245 1103 4 -0.3075401480000117 1103 5 -0.3399205916138506 1103 6 0.07052591268978148 1103 7 0.12539205293898448 1103 8 0.07901842922282754 1103 9 -0.10268187065706513 1103 10-11 -0.14351546629665535 1103 12-13 0.04248567443796247 1103 14-15 -0.021760684841062528 1103 16-17 0.0033561166501954176 1103 18-19 -0.07369938217895822 1103 20-21 -0.06316874762591596 1103 22-23 0.036638695219963324 1103 24-25 0.029664373004727906 1103 26-27 0.07699308740169641 1103 28-29 0.020446461423418327 1103 30-31 0.023572555104756532 1103 32-33 0.09671482877215709 1103 34-35 0.08983896938629954 1103 36-37 0.007027522048900892 1103 38-39 0.10715385771062813 1103 40-41 0.06368231012594094 1103 42-43 -0.17717418360749804 1103 44-45 0.12047491741761718 1103 46-47 0.11542568599714542 1103 48-49 -0.10241685029992453 1103 50-51 -0.1934350054235523 1103 52-53 -0.1637007425436039 1103 54-55 -0.260827711312146 1103 56-57 -0.08754748627469411 1103 58-59 -0.2649102531038281 1103 60-61 -0.2702916280945935 1103 62-63 -0.20024369023916577 1103 64-65 -0.17237700679872603 1103 66-67 -0.14421615978017854 1103 68-69 -0.2656130970424506 1103 70-71 -0.4031804763617579 1103 72-73 -0.03559991722535116 1103 74-75 -0.3025925650748462 1103 76-77 -0.3378015495939444 1103 78-79 -0.383796972177759 1103 80-81 -0.24582958401733634 1103 82-83 -0.21849215844717307 1103 84-85 -0.17392359817079495 1103 86-87 -0.2543213303704839 1103 88-89 -0.26418701439526515 1103 90-91 -0.25785870241541886 1103 92-93 -0.21545199816872795 1103 94-95 -0.2923110134035021 1103 96-97 -0.23837936590637554 1103 98-99 -0.36905322827045595 1103 100-101 -0.37793672861375427 1103 102-103 -0.29858002662908234 1103 104-105 -0.18056276939373817 1103 106-107 -0.3611467961685513 1103 108-109 -0.28405987965491164 1103 110-111 -0.40446333637570575 1103 112-113 -0.5720229164234247 1103 114-115 -0.4888473947207075 1103 116-117 -0.3973808186010608 1103 118-119 -0.23492083805927777 1103 120-121 -0.41512034463778136 1103 122-123 -0.5360024821094171 1103 124-125 -0.7164184648118805 1104 1 -0.4949715038753091 1104 2 -0.20840502021057716 1104 3 -0.3545736921497138 1104 4 -0.25933964909428653 1104 5 -0.3439289500445035 1104 6 -0.08828426969883196 1104 7 -0.10765881734480587 1104 8 -0.16009593051362714 1104 9 -0.19508250071767463 1104 10-11 -0.15330508157325795 1104 12-13 -0.07826795261240704 1104 14-15 -0.06697361221674214 1104 16-17 -0.020553658339437675 1104 18-19 -0.02449421240663696 1104 20-21 -0.12753304505302765 1104 22-23 -0.0985911706012601 1104 24-25 -0.170747263723527 1104 26-27 0.05901720022075807 1104 28-29 -0.06648225883957792 1104 30-31 -0.22495769850684866 1104 32-33 -0.17316337890220979 1104 34-35 -0.13322992571637116 1104 36-37 -0.13292232162108064 1104 38-39 -0.016658448941853976 1104 40-41 -0.04742369333465035 1104 42-43 0.014286051178437731 1104 44-45 -0.05903130525695843 1104 46-47 -0.07378261360774019 1104 48-49 -0.22297484036921844 1104 50-51 -0.26686477399191944 1104 52-53 -0.29523698395765763 1104 54-55 -0.0984265263930908 1104 56-57 -0.042260308424786786 1104 58-59 -0.16806137319961678 1104 60-61 -0.04208921501702534 1104 62-63 -0.05925146687649985 1104 64-65 -0.1069101302315687 1104 66-67 0.030185410128034107 1104 68-69 0.00930193240275301 1104 70-71 -0.08855989359160077 1104 72-73 -0.07740459520275067 1104 74-75 -0.11034722355139692 1104 76-77 -0.18159381490318438 1104 78-79 -0.23390802467060112 1104 80-81 0.012347799377995017 1104 82-83 -0.05153835104087179 1104 84-85 -0.06972912872679515 1104 86-87 -0.21816134941882837 1104 88-89 -0.28245011713493895 1104 90-91 -0.13450603941812034 1104 92-93 0.004741554052966279 1104 94-95 -0.054696028418799614 1104 96-97 -0.19485453697875244 1104 98-99 -0.27085755968578695 1104 100-101 -0.20393904716786082 1104 102-103 -0.12792639979419107 1104 104-105 -0.050613306403334946 1104 106-107 -0.3040836457361884 1104 108-109 -0.29072289455097433 1104 110-111 -0.30823072642934335 1104 112-113 -0.2173133174801336 1104 114-115 -0.2721807849165572 1104 116-117 -0.24192658706024872 1104 118-119 -0.30271030896494366 1104 120-121 -0.2665727988359201 1104 122-123 -0.3990363941954165 1104 124-125 -0.6978853399425162 1105 1 -0.1028676506543782 1105 2 -0.04705239016012541 1105 3 -0.05769103542135667 1105 4 -0.08441466120643071 1105 5 -0.2747822167188865 1105 6 0.12042510610474721 1105 7 0.12587118091548177 1105 8 0.0708888716061935 1105 9 0.07014223758032756 1105 10-11 -0.09006371099852117 1105 12-13 0.06174313372377327 1105 14-15 -0.06373811923693751 1105 16-17 0.08232699564003099 1105 18-19 0.023299194290352432 1105 20-21 0.10181341213652217 1105 22-23 0.2304085050220479 1105 24-25 -0.0726493033460045 1105 26-27 -0.07025913130054562 1105 28-29 0.07481169229737361 1105 30-31 0.013546162476941959 1105 32-33 -0.023973229002741903 1105 34-35 0.04703029648167245 1105 36-37 -0.15835695521056437 1105 38-39 -0.13773356883301346 1105 40-41 -0.13946627540092038 1105 42-43 -0.05155856371780487 1105 44-45 0.007743163524217778 1105 46-47 -0.10710525856703867 1105 48-49 -0.14353304554025215 1105 50-51 -0.1837547673172395 1105 52-53 -0.1592313520711528 1105 54-55 -0.21786338799305582 1105 56-57 -0.21499363092245716 1105 58-59 -0.20028630106391887 1105 60-61 -0.23190893296178317 1105 62-63 -0.12029077537190958 1105 64-65 -0.15167069274789924 1105 66-67 -0.2044387963446681 1105 68-69 -0.20793870659162295 1105 70-71 -0.06964038857104171 1105 72-73 -0.15479932525964912 1105 74-75 -0.21904495506313637 1105 76-77 -0.27453865630862495 1105 78-79 -0.1976803714875146 1105 80-81 -0.14874868224377735 1105 82-83 -0.049085035640668906 1105 84-85 -0.20727381716228166 1105 86-87 -0.10909600830692057 1105 88-89 -0.27225321770488264 1105 90-91 -0.07574074675478926 1105 92-93 0.04027722093472974 1105 94-95 -0.14013723271581924 1105 96-97 -0.061373741049816743 1105 98-99 -0.16948680820616602 1105 100-101 -0.15330110632224958 1105 102-103 0.0430291321801306 1105 104-105 -0.0026823168533951502 1105 106-107 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0.46228396832431073 2315 106-107 0.6350439911200567 2315 108-109 0.5460698449103099 2315 110-111 0.6281370452934922 2315 112-113 0.6450406345507567 2315 114-115 0.5123861640258305 2315 116-117 0.6503561829171431 2315 118-119 0.6819754902162387 2315 120-121 0.7413546777543907 2315 122-123 0.7702102568545435 2315 124-125 0.9752453775583731 2316 1 -0.39725957059699013 2316 2 -0.77102876627659 2316 3 -0.20900988075519322 2316 4 0.1622951639099135 2316 5 0.03541530170559071 2316 6 -0.6155327219905047 2316 7 -0.5528006762264397 2316 8 -0.24921820025573282 2316 9 -0.6012943571204659 2316 10-11 -0.564991377647587 2316 12-13 -0.4294765457274963 2316 14-15 -0.2919665129971136 2316 16-17 -0.3554028646388332 2316 18-19 -0.466103719242291 2316 20-21 -0.29995151777162476 2316 22-23 -0.25255441782353927 2316 24-25 -0.37655677871739357 2316 26-27 -0.11856997505858402 2316 28-29 0.12172785923495866 2316 30-31 0.09198009820915587 2316 32-33 -0.16836846398302185 2316 34-35 -0.23398785566237024 2316 36-37 -0.20345091147288485 2316 38-39 -0.21005786123629377 2316 40-41 -0.04772217433698245 2316 42-43 0.04848565927013482 2316 44-45 -0.04177863265570636 2316 46-47 -0.044530887046143164 2316 48-49 -0.1334795674297098 2316 50-51 -0.07282898137385274 2316 52-53 -0.19785236000449657 2316 54-55 -0.09200094817929738 2316 56-57 -0.1585763154785056 2316 58-59 -0.009505401540014446 2316 60-61 -0.13528078883165406 2316 62-63 -0.15578673391697961 2316 64-65 -0.11991523382526381 2316 66-67 -0.04561876280111221 2316 68-69 -0.011571342212317148 2316 70-71 -0.10217647140689223 2316 72-73 0.036127811754326444 2316 74-75 -0.04896015370135132 2316 76-77 -0.008100261465283864 2316 78-79 0.00942125831606333 2316 80-81 -0.22690002065168713 2316 82-83 -0.21616317493315762 2316 84-85 -0.1682260944967915 2316 86-87 -0.03880289922945934 2316 88-89 0.06115799652788567 2316 90-91 -0.10100595940481938 2316 92-93 -0.15291662126716332 2316 94-95 -0.07747001123005859 2316 96-97 -0.09698355764728461 2316 98-99 -0.066939731780284 2316 100-101 -0.10654755932831605 2316 102-103 -0.12322993261690485 2316 104-105 -0.18772473198939466 2316 106-107 0.20017353271230576 2316 108-109 0.06083340343739607 2316 110-111 0.10557905998220463 2316 112-113 0.039998878743869426 2316 114-115 -0.0761476013597644 2316 116-117 -0.116136847903622 2316 118-119 0.06860888293206457 2316 120-121 -0.04798664960953758 2316 122-123 0.04751746417161584 2316 124-125 0.13945643767070237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 4.0 11 29.0 12 88.0 13 251.0 14 657.0 15 4654.0 16 10380.0 17 14162.0 18 19408.0 19 27762.0 20 46194.0 21 79208.0 22 130152.0 23 187923.0 24 186400.0 25 127192.0 26 89903.0 27 79035.0 28 71471.0 29 74237.0 30 84193.0 31 98164.0 32 119479.0 33 152080.0 34 200905.0 35 271997.0 36 432381.0 37 909423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.25061448956283 15.356277987432806 10.47905726418789 23.91405025881647 2 14.818061137170572 22.712975113638624 37.06334146138745 25.405622287803354 3 20.157835617937387 32.06657746523792 26.32648600695256 21.44910090987213 4 12.986017034698468 18.694718424217243 34.41196349623873 33.90730104484555 5 13.288061943996848 38.92058640535531 30.63363361429923 17.15771803634861 6 28.35906140122707 35.046476714243035 19.845055187166462 16.74940669736343 7 26.199823099112773 35.047793376486695 21.086667682934856 17.665715841465673 8 28.923675430468094 29.541892242606426 20.83070854276797 20.70372378415751 9 31.388818260525326 14.729207928179294 20.0000994811473 33.88187433014808 10-11 24.16538974811666 26.968944033954674 25.831889149913117 23.033777068015553 12-13 27.277277072258133 25.23092061316668 28.258746367840903 19.233055946734282 14-15 25.78336283144412 23.490775903208355 29.056907019945676 21.66895424540185 16-17 20.512822388407752 27.401760172605645 29.23547567934651 22.84994175964009 18-19 20.820665313525627 25.427133296486232 34.09176625283412 19.66043513715402 20-21 23.109969217742083 24.507086678221167 33.265715066101905 19.117229037934845 22-23 21.742526405661298 26.353536101269466 31.35711595961417 20.54682153345507 24-25 20.562458524641773 27.500181408974246 32.09633869874758 19.841021367636404 26-27 21.213771717731195 26.8416593627471 32.31707540088168 19.627493518640026 28-29 20.813824015505144 27.459351737401523 32.42582850565343 19.300995741439905 30-31 21.714589592060808 27.568537866169844 31.654630159952774 19.06224238181657 32-33 19.343421501913696 28.28167092046102 32.15022940645159 20.2246781711737 34-35 18.958882978863475 29.33258683460645 31.69492760259505 20.013602583935022 36-37 20.212184509439442 29.527774697438332 30.55221107090577 19.707829722216452 38-39 20.0067647506749 28.972487917005402 30.448488047501893 20.5722592848178 40-41 19.403631620418743 29.6668405651398 30.386666132194634 20.54286168224682 42-43 20.011214341440002 29.500910431319134 30.126109966599167 20.361765260641697 44-45 20.20365800108486 29.03495800272859 30.120670242168217 20.640713754018332 46-47 19.331781507501788 28.732244589706145 30.734722169022216 21.20125173376985 48-49 19.24701864583827 29.041562023952398 30.19325688733871 21.51816244287062 50-51 18.41183376958413 28.66007033342399 30.090487609161165 22.83760828783071 52-53 18.747204682760945 27.732717545220105 29.765073722983125 23.755004049035826 54-55 19.186292888117585 27.31398446166034 29.813112193306118 23.68661045691596 56-57 19.634394002223846 26.217900117797733 29.964880862822525 24.182825017155903 58-59 19.058489026151694 26.32697546883991 30.54619702431979 24.06833848068861 60-61 19.612861568655433 26.044879129900895 30.08856378504035 24.25369551640333 62-63 18.850770653410095 26.10102414671445 30.761410648583187 24.286794551292264 64-65 18.63036223681564 26.162415636391934 30.690118905523033 24.517103221269394 66-67 19.010920837692492 25.895828056457976 30.627358992713322 24.46589211313621 68-69 19.332227344571105 25.574200983817974 30.623416113121593 24.470155558489324 70-71 18.851303631550277 25.47936375556303 30.68591707913705 24.983415533749646 72-73 19.28697695856459 25.505716785678572 30.405764257311223 24.801541998445607 74-75 19.493311270570764 25.321410865499384 30.285884883600716 24.899392980329132 76-77 19.027922499762116 25.468253119827455 29.727186169969066 25.776638210441362 78-79 19.394105851091815 25.388226573780905 29.27538583124734 25.942281743879942 80-81 18.888108748610836 25.805023808998108 28.403170607847034 26.90369683454402 82-83 18.989945215921082 25.673911859877542 27.57010611012439 27.766036814076994 84-85 19.570848795819867 25.55268904953678 27.037484906470493 27.838977248172853 86-87 19.562194084340426 25.382521844056033 26.741941160980943 28.313342910622602 88-89 19.73082941913273 25.613828163046403 26.22563819253904 28.429704225281828 90-91 20.33125952276751 25.914950209085696 25.676710008473464 28.07708025967333 92-93 20.007803521100982 26.70824640386516 25.007884045239926 28.276066029793935 94-95 19.789652965869482 27.028106057436318 25.26357383680937 27.91866713988483 96-97 20.218723931916273 27.212394882806816 24.807087036933545 27.76179414834336 98-99 20.46526162225077 27.657997274801748 24.381263837754442 27.495477265193042 100-101 20.56518165696384 27.672568336964883 24.407904303817766 27.354345702253514 102-103 20.40092072727741 28.41719935407476 24.65338866435734 26.528491254290493 104-105 20.299552364096318 28.61842923364698 24.88728639715273 26.194732005103965 106-107 19.709863233903878 28.81818152559021 25.26674845577463 26.20520678473128 108-109 19.66585745580477 29.124861421298853 25.37110125337468 25.8381798695217 110-111 19.43422730798456 29.263257252687673 25.791818728964493 25.510696710363273 112-113 18.930114004149363 29.460443668456065 25.9150645419735 25.694377785421075 114-115 19.04242867893388 29.14242703788708 26.35206138921645 25.46308289396259 116-117 18.852162747462646 29.370419539119247 26.68723516821109 25.090182545207018 118-119 18.61596624425104 29.792255186149408 26.808377177485465 24.783401392114087 120-121 18.504569750273316 30.087852824581624 27.013377519280933 24.394199905864124 122-123 18.05353692638746 30.73515862006331 26.9385456202054 24.272758833343833 124-125 17.513187989676076 31.61599066972955 27.356918833844933 23.51390250674944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 124.0 1 71.0 2 39.5 3 75.0 4 121.0 5 283.5 6 728.5 7 1347.0 8 2407.5 9 3395.5 10 3791.0 11 4203.5 12 4799.5 13 5495.0 14 6310.0 15 7459.0 16 8702.5 17 10042.5 18 11746.0 19 13709.0 20 15902.5 21 18262.5 22 21280.0 23 24727.0 24 28689.5 25 33822.0 26 39875.0 27 46438.0 28 53499.0 29 61025.5 30 67969.5 31 73595.5 32 77560.5 33 78774.5 34 77082.5 35 73154.5 36 68744.0 37 65132.0 38 61740.0 39 58430.0 40 55875.0 41 55429.5 42 56223.0 43 60121.0 44 66764.5 45 73576.0 46 84247.0 47 95471.0 48 119823.0 49 147815.0 50 177064.5 51 184173.0 52 155834.0 53 142721.5 54 141556.0 55 130218.0 56 110094.0 57 88824.0 58 73383.0 59 61184.5 60 48468.5 61 38107.5 62 29743.5 63 21549.5 64 14842.0 65 11192.5 66 8460.5 67 6285.0 68 4943.5 69 4003.0 70 3415.5 71 2991.5 72 2636.0 73 2607.5 74 2104.5 75 1510.0 76 1192.5 77 865.5 78 623.5 79 403.0 80 262.5 81 203.5 82 166.5 83 123.0 84 71.0 85 40.0 86 17.0 87 7.5 88 5.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11481294764687587 2 0.022266221498285558 3 0.0 4 8.777748291045556E-5 5 0.020803263449777967 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.7555496582091112E-4 20-21 5.120353169776574E-4 22-23 0.0 24-25 0.001199625599776226 26-27 0.001389810146082213 28-29 8.338860876493278E-4 30-31 1.609253853358352E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.8277615600750555E-4 40-41 8.777748291045556E-4 42-43 0.33298388142081314 44-45 0.31731560072129683 46-47 0.3153698665167817 48-49 0.32482057551014076 50-51 0.3290046355288725 52-53 0.33257425316723105 54-55 0.3331009180646938 56-57 0.3356172059081268 58-59 0.33510517059114914 60-61 0.3526314080122701 62-63 0.34597494889156055 64-65 0.35368473780719556 66-67 0.3670708039510401 68-69 0.37851113589036944 70-71 0.3639254441467487 72-73 0.36695376730715945 74-75 0.3774724356759291 76-77 0.37267393327682413 78-79 0.4469044246580994 80-81 0.6117505375639349 82-83 0.5807358269355739 84-85 0.541630958298966 86-87 0.49680592369269333 88-89 0.32536186998808864 90-91 0.017365312035785126 92-93 0.07630789181015603 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.17214627356788842 114-115 0.15440059243949134 116-117 0.14838783486012513 118-119 0.13187103849247442 120-121 0.1340508459847507 122-123 0.12838919833702633 124-125 0.001170366438806074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3417733.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.64118291692098 #Duplication Level Percentage of deduplicated Percentage of total 1 70.47337544390267 26.527012158567874 2 15.971958580657382 12.024068289520203 3 6.0661194116270964 6.850077311068219 4 2.6894959649959196 4.049432382909292 5 1.2728796400004503 2.395634768024074 6 0.730864935272211 1.6506372429686944 7 0.46679283963012264 1.2299444262578487 8 0.3207970817584406 0.9660145306947137 9 0.23948884592771788 0.8113179110514781 >10 1.4466725383469472 10.342237753972581 >50 0.16342984686380144 4.321580788753326 >100 0.1296842465339683 9.95799575129214 >500 0.01517362926350303 3.956507871720395 >1k 0.011916462772350884 8.337493328179125 >5k 8.738739366390649E-4 2.1672428992693433 >10k+ 4.7665851089403533E-4 4.412802585750642 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55528 1.6247026903505921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25370 0.7423049138127525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20540 0.6009831663269191 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 17746 0.5192330705763147 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16919 0.49503574445399917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11466 0.3354855396837611 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 8829 0.25832913220547066 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 8223 0.2405980806575587 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8210 0.2402177115649467 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7524 0.22014592713942255 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6884 0.20142006411852537 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 6667 0.1950708261880024 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 5513 0.16130575442844716 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 5330 0.15595132797090938 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5163 0.151065048088894 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 5078 0.1485780194064311 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 5054 0.14787579954314747 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 4702 0.137576574881654 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 4528 0.1324854808728476 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4524 0.13236844422896699 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 4442 0.12996919302941454 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.1231810676843393 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4208 0.12312254936239898 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4163 0.12180588711874216 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 4056 0.11867515689493592 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3838 0.11229665980344282 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 3668 0.10732260243851699 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 3444 0.10076855038120297 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9259160970151853E-5 2 0.0 0.0 0.0 0.0 2.9259160970151853E-5 3 0.0 0.0 0.0 2.9259160970151853E-5 2.9259160970151853E-5 4 0.0 0.0 0.0 5.8518321940303705E-5 2.9259160970151853E-5 5 0.0 0.0 0.0 5.8518321940303705E-5 2.9259160970151853E-5 6 0.0 0.0 0.0 5.8518321940303705E-5 2.9259160970151853E-5 7 0.0 0.0 0.0 8.777748291045556E-5 2.9259160970151853E-5 8 0.0 0.0 0.0 8.777748291045556E-5 2.9259160970151853E-5 9 0.0 0.0 0.0 3.2185077067167035E-4 8.777748291045556E-5 10-11 5.8518321940303705E-5 0.0 0.0 7.607381852239481E-4 8.777748291045556E-5 12-13 5.8518321940303705E-5 0.0 0.0 0.0015214763704478962 8.777748291045556E-5 14-15 5.8518321940303705E-5 0.0 0.0 0.002179807492276313 8.777748291045556E-5 16-17 5.8518321940303705E-5 0.0 0.0 0.004403503726007854 8.777748291045556E-5 18-19 8.777748291045556E-5 0.0 0.0 0.006685718281679699 1.7555496582091112E-4 20-21 1.1703664388060741E-4 0.0 0.0 0.011264776973508464 1.7555496582091112E-4 22-23 1.9018454630598703E-4 0.0 0.0 0.028410645302017448 2.0481412679106297E-4 24-25 2.6333244873136665E-4 0.0 0.0 0.06393126671978179 2.3407328776121482E-4 26-27 2.6333244873136665E-4 0.0 0.0 0.10887333796993504 2.3407328776121482E-4 28-29 3.2185077067167035E-4 0.0 0.0 0.21449890907218322 2.3407328776121482E-4 30-31 3.2185077067167035E-4 0.0 0.0 0.4617827080114216 2.3407328776121482E-4 32-33 3.8036909261197406E-4 0.0 0.0 0.878052791133772 2.3407328776121482E-4 34-35 4.3888741455227776E-4 0.0 0.0 1.2083448297453312 2.3407328776121482E-4 36-37 4.3888741455227776E-4 0.0 0.0 1.6112727354652923 2.3407328776121482E-4 38-39 4.3888741455227776E-4 0.0 0.0 2.128106554842055 2.3407328776121482E-4 40-41 4.3888741455227776E-4 0.0 0.0 2.8614289062369704 2.3407328776121482E-4 42-43 4.3888741455227776E-4 0.0 0.0 3.5170535556756484 2.3407328776121482E-4 44-45 5.120353169776574E-4 0.0 0.0 4.100803076191148 2.3407328776121482E-4 46-47 6.144423803731889E-4 0.0 0.0 4.6905507247055285 2.4870286824629073E-4 48-49 6.437015413433407E-4 0.0 0.0 5.235239265325875 2.6333244873136665E-4 50-51 6.437015413433407E-4 0.0 0.0 5.780015583429133 2.6333244873136665E-4 52-53 6.583311218284167E-4 0.0 0.0 6.412042719545383 2.9259160970151847E-4 54-55 6.729607023134926E-4 0.0 0.0 7.004687024995809 3.2185077067167035E-4 56-57 6.729607023134926E-4 0.0 0.0 7.558182573068171 3.2185077067167035E-4 58-59 6.729607023134926E-4 0.0 0.0 8.152699464820687 3.2185077067167035E-4 60-61 6.729607023134926E-4 0.0 0.0 8.711329995643311 3.2185077067167035E-4 62-63 7.314790242537963E-4 0.0 0.0 9.278255498600974 3.5110993164182223E-4 64-65 7.314790242537963E-4 0.0 0.0 9.876385896733304 3.5110993164182223E-4 66-67 7.314790242537963E-4 0.0 0.0 10.440239187789098 3.5110993164182223E-4 68-69 7.314790242537963E-4 0.0 0.0 11.048420107714676 3.5110993164182223E-4 70-71 7.314790242537963E-4 0.0 0.0 11.738102420522608 3.8036909261197406E-4 72-73 7.314790242537963E-4 0.0 0.0 12.365214017595875 4.3888741455227776E-4 74-75 7.314790242537963E-4 0.0 0.0 12.95437648289085 4.3888741455227776E-4 76-77 7.607381852239481E-4 0.0 0.0 13.548542264711726 4.3888741455227776E-4 78-79 7.607381852239481E-4 0.0 0.0 14.134954368875508 4.3888741455227776E-4 80-81 7.607381852239481E-4 0.0 0.0 14.735176797017203 4.535169950373537E-4 82-83 7.607381852239481E-4 0.0 0.0 15.418319687348308 5.266648974627333E-4 84-85 7.899973461941E-4 0.0 0.0 16.033961693321274 5.266648974627333E-4 86-87 7.899973461941E-4 0.0 0.0 16.629824506478418 5.266648974627333E-4 88-89 7.899973461941E-4 0.0 0.0 17.214788282174176 5.412944779478093E-4 90-91 7.899973461941E-4 0.0 0.0 17.784142295492366 5.559240584328852E-4 92-93 7.899973461941E-4 0.0 0.0 18.34338726869536 5.85183219403037E-4 94-95 7.899973461941E-4 0.0 0.0 18.928716783903248 5.85183219403037E-4 96-97 7.899973461941E-4 0.0 0.0 19.50030912303565 6.290719608582648E-4 98-99 7.899973461941E-4 0.0 0.0 20.077416814010924 6.437015413433407E-4 100-101 7.899973461941E-4 0.0 0.0 20.705962109971725 6.437015413433407E-4 102-103 7.899973461941E-4 0.0 0.0 21.27162946900767 6.875902827985685E-4 104-105 7.899973461941E-4 0.0 0.0 21.82249169259272 7.314790242537963E-4 106-107 7.899973461941E-4 0.0 0.0 22.37519724331889 7.314790242537963E-4 108-109 7.899973461941E-4 0.0 0.0 22.936812208560468 7.314790242537963E-4 110-111 7.899973461941E-4 0.0 0.0 23.482875929746413 7.314790242537963E-4 112-113 7.899973461941E-4 0.0 0.0 24.06001287988266 7.314790242537963E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 1040 0.0 62.306473 1 AGATTAC 1695 0.0 50.450127 2 ATAGTAC 1420 0.0 50.174847 4 TAGTACT 1555 0.0 46.964302 5 TACAACG 660 0.0 45.88763 2 AGTGTAC 1520 0.0 44.92079 3 GATTACT 1900 0.0 44.686424 3 GACCGTT 710 0.0 40.13577 7 GCATAGT 1735 0.0 39.018883 2 TAACGCA 935 0.0 38.10069 4 GTGTACT 1905 0.0 37.089 4 TCACGCA 645 0.0 36.820824 4 GTATAAC 905 0.0 36.12885 1 ATAACGC 975 0.0 34.7107 3 CATAGTA 2065 0.0 34.5028 3 GGTTTCA 1885 0.0 34.060608 1 CATGGGT 3995 0.0 33.43943 4 ATTACTT 2700 0.0 32.32561 4 ACAACGC 965 0.0 31.994051 3 TTAGGAC 970 0.0 31.829132 3 >>END_MODULE