##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557322.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1021185 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.330800001958508 33.0 32.0 33.0 27.0 34.0 2 31.694784000940086 33.0 32.0 34.0 27.0 34.0 3 31.898744106112016 33.0 32.0 34.0 27.0 34.0 4 31.770681120462992 33.0 32.0 34.0 27.0 34.0 5 31.933461615671987 33.0 32.0 34.0 28.0 34.0 6 35.2757913600376 38.0 36.0 38.0 29.0 38.0 7 35.62182366564335 38.0 36.0 38.0 30.0 38.0 8 35.838106709362165 38.0 37.0 38.0 31.0 38.0 9 35.94385346435759 38.0 37.0 38.0 31.0 38.0 10-11 35.945141184016606 38.0 37.0 38.0 31.0 38.0 12-13 35.90009988395835 38.0 37.0 38.0 31.0 38.0 14-15 35.87496486924505 38.0 37.0 38.0 31.0 38.0 16-17 35.846882298506145 38.0 37.0 38.0 31.0 38.0 18-19 35.81155422376945 38.0 37.0 38.0 31.0 38.0 20-21 35.79002237596518 38.0 37.0 38.0 31.0 38.0 22-23 35.692276130182094 38.0 37.0 38.0 29.0 38.0 24-25 35.61871453262631 38.0 37.0 38.0 29.0 38.0 26-27 35.372707198010154 38.0 36.0 38.0 28.5 38.0 28-29 35.366828733285345 38.0 36.0 38.0 28.0 38.0 30-31 35.27570371676043 38.0 36.0 38.0 28.0 38.0 32-33 35.193711227642396 38.0 36.0 38.0 27.5 38.0 34-35 34.95132909316138 38.0 36.0 38.0 26.5 38.0 36-37 34.75751798156064 38.0 36.0 38.0 25.0 38.0 38-39 34.547626042294 38.0 35.0 38.0 24.0 38.0 40-41 34.25318575968116 38.0 35.0 38.0 16.0 38.0 42-43 33.74777684748601 38.0 34.0 38.0 16.0 38.0 44-45 33.39715330718724 38.0 33.5 38.0 15.0 38.0 46-47 32.88898142843853 38.0 32.0 38.0 15.0 38.0 48-49 32.41511626198975 38.0 29.5 38.0 15.0 38.0 50-51 32.01449002874112 38.0 27.5 38.0 15.0 38.0 52-53 31.71196551065674 38.0 26.0 38.0 15.0 38.0 54-55 31.49232362402503 38.0 25.0 38.0 15.0 38.0 56-57 31.240747758731278 38.0 25.0 38.0 15.0 38.0 58-59 31.10236685811092 38.0 24.5 38.0 15.0 38.0 60-61 30.96385522701567 38.0 24.0 38.0 15.0 38.0 62-63 30.843972933405798 38.0 24.0 38.0 15.0 38.0 64-65 30.6414963987916 37.0 24.0 38.0 15.0 38.0 66-67 30.520273505780047 37.0 20.0 38.0 15.0 38.0 68-69 30.35618227843143 37.0 16.0 38.0 14.5 38.0 70-71 30.19677335644374 37.0 16.0 38.0 14.0 38.0 72-73 29.971318125511047 37.0 15.5 38.0 14.0 38.0 74-75 29.840175384479796 37.0 15.0 38.0 14.0 38.0 76-77 29.726166169695013 37.0 15.0 38.0 14.0 38.0 78-79 29.563236827802996 37.0 15.0 38.0 14.0 38.0 80-81 29.39109270112663 37.0 15.0 38.0 14.0 38.0 82-83 29.258162820644642 36.0 15.0 38.0 14.0 38.0 84-85 29.154034773327066 36.0 14.0 38.0 13.0 38.0 86-87 29.10029132821183 36.0 14.0 38.0 13.0 38.0 88-89 28.98527788794391 36.0 14.0 38.0 13.0 38.0 90-91 28.887646704563814 36.0 14.0 38.0 13.0 38.0 92-93 28.824040697816752 36.0 14.0 38.0 13.0 38.0 94-95 28.777372856044693 35.5 14.0 38.0 13.0 38.0 96-97 28.725838119439672 35.0 14.0 38.0 13.0 38.0 98-99 28.63792897467158 35.0 14.0 38.0 13.0 38.0 100-101 28.55608533223657 35.0 14.0 38.0 13.0 38.0 102-103 28.429245435449992 35.0 14.0 38.0 13.0 38.0 104-105 28.35992743724203 34.5 14.0 38.0 13.0 38.0 106-107 28.18126196526584 34.0 14.0 38.0 13.0 38.0 108-109 28.052171252025833 34.0 14.0 38.0 13.0 38.0 110-111 27.871038058725894 34.0 14.0 38.0 13.0 38.0 112-113 27.703678569505037 34.0 14.0 38.0 13.0 38.0 114-115 27.551603774046818 34.0 14.0 38.0 13.0 38.0 116-117 27.389341794092157 33.0 14.0 38.0 13.0 38.0 118-119 27.273708485729813 33.0 14.0 38.0 13.0 38.0 120-121 27.060104192678114 31.0 14.0 38.0 13.0 38.0 122-123 26.994951453458484 31.0 14.0 38.0 13.0 38.0 124-125 25.936636358740092 27.0 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.6190264220126771 1101 2 -0.6714587207770997 1101 3 -0.567320305847062 1101 4 -0.40846485022330725 1101 5 -0.7580362550291326 1101 6 -0.146288755598178 1101 7 0.03280142973932243 1101 8 0.09610799023368344 1101 9 0.03048194174018448 1101 10-11 0.03404744373964519 1101 12-13 0.012917229734945579 1101 14-15 0.03729614803902592 1101 16-17 -0.00963967917984121 1101 18-19 0.031945189769835736 1101 20-21 -0.03929142456399859 1101 22-23 0.08910301091381712 1101 24-25 -0.10179999973535558 1101 26-27 -0.14039346581318313 1101 28-29 -0.047804032897154514 1101 30-31 -0.18641240682285343 1101 32-33 -0.04349462698970541 1101 34-35 0.15423028140447315 1101 36-37 -0.05274921862829984 1101 38-39 -0.15005551938882178 1101 40-41 0.036338505383127995 1101 42-43 0.09965940857498623 1101 44-45 0.21315104592871137 1101 46-47 0.2681337403825097 1101 48-49 0.11073922782451717 1101 50-51 0.03250527835051997 1101 52-53 0.08161266279177681 1101 54-55 0.047879408247585076 1101 56-57 0.28964885621531167 1101 58-59 0.09811340739637942 1101 60-61 0.16972839151972963 1101 62-63 0.08835311176965277 1101 64-65 0.05836592481138325 1101 66-67 -0.1499064113516404 1101 68-69 -0.009533827189361688 1101 70-71 0.17429704243431487 1101 72-73 -0.00234320450182679 1101 74-75 -0.09390287492811211 1101 76-77 0.0644991860828128 1101 78-79 0.028219350508653207 1101 80-81 0.11652774636874952 1101 82-83 0.04987121087557611 1101 84-85 0.085517903253443 1101 86-87 0.048610648409948 1101 88-89 -0.0022914620366130123 1101 90-91 0.1945111137314548 1101 92-93 -0.03229729765791234 1101 94-95 -0.08380545427421282 1101 96-97 -0.15850010985965213 1101 98-99 -0.013098854187500564 1101 100-101 0.057008374765374015 1101 102-103 -0.014490740427966387 1101 104-105 0.21523828522896693 1101 106-107 0.06472562787017111 1101 108-109 -0.34895016181360816 1101 110-111 -0.1490397372988319 1101 112-113 -0.18271406589931516 1101 114-115 -0.21819862055099648 1101 116-117 0.045013527448144686 1101 118-119 -0.17364372565878838 1101 120-121 -0.6101209047944529 1101 122-123 -0.3291395171697715 1101 124-125 -0.46364384343874576 1102 1 -0.6255564977259489 1102 2 -0.8662462526693133 1102 3 -0.6445530193631477 1102 4 -0.4156076704004015 1102 5 -0.5404581482232196 1102 6 -0.008417111072773764 1102 7 0.096896363729563 1102 8 0.01920648994128271 1102 9 -0.10817322906751059 1102 10-11 -0.04046000597028865 1102 12-13 -0.0604656475764358 1102 14-15 0.08286642993252968 1102 16-17 -0.05677804867924863 1102 18-19 -0.09931150565372349 1102 20-21 -0.03991089679642812 1102 22-23 0.07451151584837135 1102 24-25 -0.016918330834727158 1102 26-27 0.27412454131825825 1102 28-29 0.13000933880171317 1102 30-31 0.1981318386061517 1102 32-33 0.10046254300029744 1102 34-35 0.08581767253107131 1102 36-37 0.14407579211025734 1102 38-39 0.4245487066849023 1102 40-41 0.31907867378435384 1102 42-43 0.351205501552748 1102 44-45 0.33823061473049876 1102 46-47 0.462713746489257 1102 48-49 0.4664177232884512 1102 50-51 0.3054668938430254 1102 52-53 0.3413231617334489 1102 54-55 0.18544830318571215 1102 56-57 0.022794502055219823 1102 58-59 0.02154570793097932 1102 60-61 0.3077288986899802 1102 62-63 0.23074581679840023 1102 64-65 0.4112363531632326 1102 66-67 0.3389104846629323 1102 68-69 -0.011906659424688826 1102 70-71 1.756631382434648E-4 1102 72-73 -0.10736833012996527 1102 74-75 -0.24910525138299278 1102 76-77 -0.10689071915915349 1102 78-79 0.1724994533917581 1102 80-81 -0.0022525609350161346 1102 82-83 0.12793868521949392 1102 84-85 0.006325856510979833 1102 86-87 0.2243368592789139 1102 88-89 -0.05439802989133469 1102 90-91 -0.11264464446219336 1102 92-93 -0.2934439164366651 1102 94-95 -0.11518997730129499 1102 96-97 -0.2534703265455569 1102 98-99 -0.18985648373585917 1102 100-101 -0.32257802272154734 1102 102-103 -0.1662417715878881 1102 104-105 -0.21422002589410383 1102 106-107 -0.3281098160849254 1102 108-109 -0.5143937546490527 1102 110-111 -0.60084769573157 1102 112-113 -0.6561067761032753 1102 114-115 -0.4499645563059822 1102 116-117 -0.533265612846531 1102 118-119 -0.5343199181557736 1102 120-121 -0.5670155738141105 1102 122-123 -0.6563290177105792 1102 124-125 -0.5745060846137235 1103 1 -0.6502217006325104 1103 2 -0.3687456315009072 1103 3 -0.25017142420268357 1103 4 -0.6443436814343819 1103 5 -0.4300088279589289 1103 6 -0.04371367461703102 1103 7 0.03917080353707547 1103 8 -0.020707509926872092 1103 9 -0.28953310615222705 1103 10-11 -0.18772762560224976 1103 12-13 0.04881029824753824 1103 14-15 -0.1252985118297616 1103 16-17 0.01138060533756402 1103 18-19 -0.05466800561062968 1103 20-21 -0.010798635185651051 1103 22-23 0.032074304564915224 1103 24-25 -0.11503809210322657 1103 26-27 -0.09620794857825388 1103 28-29 -0.19595042976868626 1103 30-31 0.03804228894055939 1103 32-33 0.3119858507600881 1103 34-35 0.163168604790755 1103 36-37 0.08359528665665295 1103 38-39 -0.06249143854173411 1103 40-41 0.22396587467274998 1103 42-43 -0.11217345174045334 1103 44-45 -0.07304625397225095 1103 46-47 -0.005069164026991757 1103 48-49 -0.017834516720881766 1103 50-51 -0.10532115335563574 1103 52-53 -0.18410214847044415 1103 54-55 -0.07255361356250845 1103 56-57 0.07192258444496957 1103 58-59 -0.20989606196796373 1103 60-61 0.05873823396372302 1103 62-63 0.18916100068960162 1103 64-65 0.26161691388814745 1103 66-67 0.21233106891618547 1103 68-69 0.32156189424172865 1103 70-71 0.005653607856789478 1103 72-73 0.08696052910059393 1103 74-75 0.1141139728917615 1103 76-77 -0.056786514989973114 1103 78-79 -0.23863340565625535 1103 80-81 -0.042895736449320765 1103 82-83 -0.052065269975248185 1103 84-85 -0.01875058523356543 1103 86-87 -0.10170252026258453 1103 88-89 -0.15899011240277972 1103 90-91 -0.09326216191829317 1103 92-93 -0.0051705617723243336 1103 94-95 -0.051874408539219985 1103 96-97 -0.16732567797475184 1103 98-99 -0.32118314732626274 1103 100-101 -0.1353801767814531 1103 102-103 -0.09092218678192054 1103 104-105 -0.2493690302498912 1103 106-107 -0.22157715276834367 1103 108-109 -0.46677422410024505 1103 110-111 -0.5891245999296579 1103 112-113 -0.42474715255668016 1103 114-115 -0.40110066064199046 1103 116-117 -0.12004512088115504 1103 118-119 -0.010654162536098966 1103 120-121 -0.0876774097343791 1103 122-123 -0.5167055725331302 1103 124-125 -0.9146259574390001 1104 1 -0.5722180964859582 1104 2 -0.19668133574678137 1104 3 -0.27891935003418666 1104 4 -0.2678719386966968 1104 5 -0.3718607407536503 1104 6 -0.30593317864047265 1104 7 -0.3560165308020302 1104 8 -0.430508687122817 1104 9 -0.2386408915449323 1104 10-11 -0.29876775295669233 1104 12-13 -0.2691411468804219 1104 14-15 -0.0250158103454865 1104 16-17 -0.15698438487891053 1104 18-19 -0.07059325822799423 1104 20-21 -0.018681962340259872 1104 22-23 -0.07031378946009426 1104 24-25 -0.16427989306632185 1104 26-27 -0.0641641516554543 1104 28-29 -0.08447960888366879 1104 30-31 -0.25436788989885173 1104 32-33 -0.08022280910403623 1104 34-35 0.07708491246354043 1104 36-37 -0.07006169295751619 1104 38-39 0.07376433294982121 1104 40-41 0.23911944739700886 1104 42-43 0.22824294811760382 1104 44-45 0.11002179064938389 1104 46-47 0.05386695852192247 1104 48-49 0.18819112862554732 1104 50-51 0.21597572483491234 1104 52-53 0.10041429520039458 1104 54-55 0.3021324113493229 1104 56-57 0.260202070348555 1104 58-59 0.1711656149315992 1104 60-61 0.12286745986520486 1104 62-63 0.08618361482586678 1104 64-65 0.1540595062567931 1104 66-67 0.08353833906263475 1104 68-69 0.07951064319844292 1104 70-71 0.08923293377432984 1104 72-73 0.23488665797361463 1104 74-75 0.307781836314831 1104 76-77 0.10945870971409022 1104 78-79 0.2858224534608702 1104 80-81 0.10245763049363887 1104 82-83 0.19690483226118616 1104 84-85 0.09204966217979305 1104 86-87 0.33384461416335753 1104 88-89 0.0820133985672662 1104 90-91 0.22925493200560965 1104 92-93 0.3248972984787777 1104 94-95 0.16448788154104932 1104 96-97 0.24824996449708436 1104 98-99 0.21469377245669463 1104 100-101 0.09999061615061322 1104 102-103 0.3484293245608363 1104 104-105 0.26482006651292167 1104 106-107 0.03529907512949748 1104 108-109 0.14808366190325373 1104 110-111 0.03478478889193681 1104 112-113 0.24016535073918988 1104 114-115 0.166500381348893 1104 116-117 0.3880176206095527 1104 118-119 0.37778382255169163 1104 120-121 0.10524504164036941 1104 122-123 0.2480331664913713 1104 124-125 -0.055632739499152706 1105 1 -0.16378160383903761 1105 2 -0.2278503898601194 1105 3 -0.1729815698827366 1105 4 -0.16179491731458384 1105 5 -0.1576753019530308 1105 6 -0.04815951239979199 1105 7 0.2518019596704306 1105 8 0.2753756854333105 1105 9 0.11645476527311871 1105 10-11 0.08465586464492958 1105 12-13 0.1659333376409151 1105 14-15 0.12466279037551686 1105 16-17 0.07997367108462328 1105 18-19 -0.08959301026416 1105 20-21 -0.0029591477599808513 1105 22-23 -0.05098977702375862 1105 24-25 -0.3031358260779342 1105 26-27 0.031037748129008946 1105 28-29 -0.10423469691082943 1105 30-31 0.06444398184662248 1105 32-33 0.046776637745871597 1105 34-35 0.11751255470894506 1105 36-37 0.28613072697877584 1105 38-39 0.09675439532226449 1105 40-41 0.23109018766442802 1105 42-43 0.10212758771169916 1105 44-45 0.10950202030204537 1105 46-47 0.09573565213398894 1105 48-49 0.14306552540583795 1105 50-51 -0.0011633447284751242 1105 52-53 0.07126900746697373 1105 54-55 -0.042392356423011535 1105 56-57 0.05932179873278187 1105 58-59 -0.027105314169816097 1105 60-61 -0.05629022605998557 1105 62-63 0.1496461436755041 1105 64-65 0.17192816172057945 1105 66-67 0.2863393766959135 1105 68-69 0.08727858719676718 1105 70-71 0.05102456785600751 1105 72-73 0.29620048481856287 1105 74-75 -0.04176515283198512 1105 76-77 0.08513250375151316 1105 78-79 -0.09287840678290138 1105 80-81 -0.05717767192686907 1105 82-83 0.33702538083348443 1105 84-85 0.19061624046960546 1105 86-87 0.156175664957793 1105 88-89 0.4357175967857394 1105 90-91 0.4547627810147077 1105 92-93 0.4773664460554059 1105 94-95 0.17701863859371514 1105 96-97 0.46220269877522213 1105 98-99 0.3555477217576275 1105 100-101 0.4455148689211299 1105 102-103 0.4260009956463442 1105 104-105 0.5364215314069618 1105 106-107 0.592500517134571 1105 108-109 0.5334986198447744 1105 110-111 0.40033784361564884 1105 112-113 -0.05846321850948044 1105 114-115 0.11293733177520338 1105 116-117 0.2486390561294165 1105 118-119 0.10807165134220043 1105 120-121 0.49200281912144206 1105 122-123 0.3407973910321651 1105 124-125 -0.0775260387533514 1106 1 0.17715054158772858 1106 2 0.10716058021422725 1106 3 0.010169817143260929 1106 4 -0.17535517277963564 1106 5 -0.4587145721544026 1106 6 -0.05966787541598961 1106 7 0.3178915500913817 1106 8 0.1917517413584804 1106 9 -0.024159381237325306 1106 10-11 0.03624097376830093 1106 12-13 0.0985779694106057 1106 14-15 0.06103724742345662 1106 16-17 0.15446802615972643 1106 18-19 0.15350472554802508 1106 20-21 0.06225638977365833 1106 22-23 0.09542118772100139 1106 24-25 -0.06261938873171147 1106 26-27 0.11880678143006662 1106 28-29 0.11366917503686835 1106 30-31 -0.01536841655805432 1106 32-33 0.065302295284134 1106 34-35 0.2955231072986848 1106 36-37 0.16940647112289042 1106 38-39 0.19521769664844157 1106 40-41 0.28434967655667975 1106 42-43 0.026740082049009573 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0.47549025534626566 2314 116-117 0.543895096716323 2314 118-119 0.6433725857169712 2314 120-121 0.7699993882140781 2314 122-123 0.751962439575852 2314 124-125 0.9613390243899396 2315 1 0.5704856176126505 2315 2 0.1722605671440398 2315 3 0.01312106534611246 2315 4 0.009929539458415348 2315 5 0.1719132324898709 2315 6 -0.17512206441089262 2315 7 -0.07683419382009049 2315 8 -0.3221000427220275 2315 9 -0.11293034799548707 2315 10-11 -0.23752628977023704 2315 12-13 -0.20548598737064339 2315 14-15 -0.22807877226895812 2315 16-17 -0.17665600991399089 2315 18-19 -0.16331006990354524 2315 20-21 -0.002949901342972794 2315 22-23 0.030711563706717016 2315 24-25 -0.1008427146586115 2315 26-27 -0.08671537253673733 2315 28-29 0.02749438307068175 2315 30-31 -0.13563461269799149 2315 32-33 -0.2008655259157095 2315 34-35 -0.6090662709960881 2315 36-37 -0.4530694579495673 2315 38-39 -0.5267315038917886 2315 40-41 -0.6669634415542518 2315 42-43 -0.2916588200552894 2315 44-45 -0.41768706892587915 2315 46-47 -0.2607368397753973 2315 48-49 -0.27032790963809106 2315 50-51 -0.5115563174515501 2315 52-53 -0.35979433048957077 2315 54-55 -0.3057672986329045 2315 56-57 -0.31940219571936623 2315 58-59 -0.21970818055909191 2315 60-61 -0.31197677252323075 2315 62-63 -0.2978064684373045 2315 64-65 -0.09173804262523078 2315 66-67 -0.07342021646173791 2315 68-69 -0.02588368742181757 2315 70-71 -0.2334137123104263 2315 72-73 -0.1690098711684911 2315 74-75 -0.17357282735810742 2315 76-77 -0.10211206397849182 2315 78-79 -0.3457632888910851 2315 80-81 -0.19481058917143912 2315 82-83 -0.300367129568734 2315 84-85 -0.1155095108017754 2315 86-87 -0.33964495455214205 2315 88-89 0.09471898374829024 2315 90-91 -0.30465469471344875 2315 92-93 -0.12816753452711538 2315 94-95 -0.21495547143864968 2315 96-97 -0.2793784385340672 2315 98-99 -0.4927231982608653 2315 100-101 -0.23822530676078912 2315 102-103 -0.22738319298056453 2315 104-105 -0.24914943484361274 2315 106-107 -0.11919620038738898 2315 108-109 0.03786755188361113 2315 110-111 -0.05500658541809855 2315 112-113 -0.24878684310494847 2315 114-115 -0.7845661356311773 2315 116-117 -0.5338805925067369 2315 118-119 -0.3948918253105944 2315 120-121 -0.25352191504407173 2315 122-123 -0.3808947032812888 2315 124-125 -0.15433140167521842 2316 1 -0.4024176758633331 2316 2 -0.7696022396526878 2316 3 -0.22594123798931776 2316 4 0.11717518661595605 2316 5 0.026339496387500105 2316 6 -0.5147948145682264 2316 7 -0.6258251734718598 2316 8 -0.2650075317088181 2316 9 -0.24124165489710236 2316 10-11 -0.7327706782745338 2316 12-13 -0.5807177893425362 2316 14-15 -0.39878466696163883 2316 16-17 -0.3643400879504881 2316 18-19 -0.5633730025655979 2316 20-21 -0.1963063099857223 2316 22-23 -0.23986322127336024 2316 24-25 -0.2771307364752644 2316 26-27 -0.142300646293819 2316 28-29 -0.056189275414745055 2316 30-31 -0.2900126282213833 2316 32-33 -0.19871008224044573 2316 34-35 -0.5398802000919574 2316 36-37 -0.7039274399088669 2316 38-39 -0.7488103794948984 2316 40-41 -0.3358883419108665 2316 42-43 -0.45568965705969333 2316 44-45 -0.32244058266618225 2316 46-47 -0.3907662083510246 2316 48-49 -0.5507285809198166 2316 50-51 -0.305604985376867 2316 52-53 -0.2516267198329736 2316 54-55 -0.466713386319082 2316 56-57 -0.3606923152914234 2316 58-59 -0.21860161458468141 2316 60-61 -0.5356709197856588 2316 62-63 -0.3129816310133471 2316 64-65 -0.16977664132462422 2316 66-67 -0.4568762684860701 2316 68-69 -0.3275293765344678 2316 70-71 -0.3145842598245885 2316 72-73 -0.006748490845438937 2316 74-75 -0.20142577470444678 2316 76-77 -0.12593417432042386 2316 78-79 -0.35039198618497736 2316 80-81 -0.700526972641125 2316 82-83 -0.46110344171473727 2316 84-85 -0.5888050845378778 2316 86-87 -0.6871821897105264 2316 88-89 -0.5833395436274138 2316 90-91 -0.689985091189218 2316 92-93 -0.5556785477429749 2316 94-95 -0.4541551777528916 2316 96-97 -0.6971285958657241 2316 98-99 -0.6624861519004703 2316 100-101 -0.2603157200186068 2316 102-103 -0.6471534887640757 2316 104-105 -0.772574020536922 2316 106-107 -0.7039560073938951 2316 108-109 -0.48393861551727113 2316 110-111 -0.5531521696429778 2316 112-113 -0.5817310428112634 2316 114-115 -0.7184396409804528 2316 116-117 -0.5909112197356023 2316 118-119 -0.4674447936344883 2316 120-121 -0.4948518919687608 2316 122-123 -0.11147368377216438 2316 124-125 -0.14736265823070838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 3.0 11 4.0 12 32.0 13 81.0 14 233.0 15 1572.0 16 3253.0 17 4920.0 18 6952.0 19 10153.0 20 16584.0 21 28120.0 22 44765.0 23 62571.0 24 63085.0 25 45057.0 26 30673.0 27 26613.0 28 22842.0 29 22352.0 30 24865.0 31 28575.0 32 34735.0 33 44282.0 34 58006.0 35 77639.0 36 121839.0 37 241378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.33814924623904 14.878739391657646 9.958213719861327 22.824897642241986 2 14.902125950703018 23.27379757188123 36.61767658711697 25.206399890298787 3 21.088735145933402 33.19574807698899 25.813736002781084 19.90178077429653 4 13.037917787507233 18.868998015047254 33.671927558527706 34.42115663891781 5 13.269663603650548 38.82397418995924 30.57772884961577 17.32863335677445 6 27.970348173935182 33.29905942605894 20.438901864010926 18.29169053599495 7 25.716887733368587 35.35755029695892 21.374089905355053 17.551472064317437 8 29.109025299039843 28.70684547853719 21.805451509765618 20.378677712657353 9 31.769953534374277 15.103629606780359 20.671670657128725 32.45474620171663 10-11 24.118548548989654 26.930184050882062 25.6129888316025 23.33827856852578 12-13 27.061600003917018 25.61808095496898 28.511484207073156 18.808834834040844 14-15 26.213418724325173 23.28397890685821 29.757340736497305 20.745261632319316 16-17 20.26308664105262 27.382955773418026 30.490425579315 21.863532006214353 18-19 20.554610846521857 25.175274399484127 35.37049377463132 18.89962097936269 20-21 22.38103095489089 24.50088353712545 34.79970955155289 18.318375956430774 22-23 21.21919262346453 26.628403891952875 32.206830502632236 19.945572981950363 24-25 19.848938426691657 28.08534890757981 32.856283900964875 19.209428764763658 26-27 20.57096132142219 27.113195860640587 32.95589138955465 19.359951428382573 28-29 20.25749812103244 27.77366213837525 33.47901554340721 18.489824197185097 30-31 21.342687185995466 27.94017921362811 32.659899028920854 18.05723457145557 32-33 18.55437555389082 28.896233297590545 33.05062256104428 19.498768587474355 34-35 17.886719840185666 30.125540426073634 32.50214211920465 19.485597614536054 36-37 19.468803399971602 30.413930874425297 31.027874479159017 19.08939124644408 38-39 19.402544408162907 29.83747731179747 30.89723119468106 19.862747085358563 40-41 18.509585488929954 30.57896803596627 30.713077726823602 20.198368748280174 42-43 19.436229768152906 30.263178578656863 30.41182874086533 19.888762912324896 44-45 19.569453265177515 29.522531058394648 30.630922682242893 20.277092994184947 46-47 18.581703176258344 29.400048822441033 31.366011624063272 20.652236377237347 48-49 18.444470642557853 29.71539024545995 30.78388030081984 21.056258811162362 50-51 17.534405652665995 29.47018045774648 30.54086236167002 22.454551527917506 52-53 17.72825901671074 28.700008891565638 29.861119639700007 23.710612452023618 54-55 18.224651208488897 28.036402043623504 29.870505010807623 23.868441737079976 56-57 18.89729661749267 26.493040493505234 30.23761017233087 24.372052716671227 58-59 18.522898042658383 26.34607458265928 31.042014338019825 24.089013036662514 60-61 18.94250054292978 26.112907774656136 30.746973105076812 24.197618577337277 62-63 17.889874451332574 26.275703969664722 31.565069230111636 24.269352348891072 64-65 17.678955641081956 26.25136409040904 31.44412032198676 24.62555994652225 66-67 18.49749727024076 25.884211033239673 31.081515377561818 24.53677631895775 68-69 18.827275470496364 25.458013353869685 31.229979270343616 24.48473190529034 70-71 18.225918123758323 25.63233183312228 31.171368523675376 24.970381519444025 72-73 18.806624665049306 25.32131433842047 30.90337547745677 24.968685519073457 74-75 19.250502751124923 24.79486967786578 30.735490985863994 25.219136585145304 76-77 18.79724521859468 25.023023674332705 30.279989502973663 25.899741604098953 78-79 18.899163151931006 25.231638129111456 29.656614916118475 26.212583802839067 80-81 18.39664234799186 25.578500386701414 28.846841610057194 27.17801565524953 82-83 18.500804438471413 25.664369654620856 27.64756779862593 28.1872581082818 84-85 19.00711349595097 25.377999852314964 27.134024171117733 28.480862480616338 86-87 19.27562281686274 25.009754770242566 26.703615882980152 29.011006529914546 88-89 19.566094115115824 25.160911923471602 26.246167857306013 29.02682610410656 90-91 20.140989226248777 25.633006856023506 25.72296767874633 28.503036238981387 92-93 19.60908160785267 26.56508479094309 25.01808128640758 28.807752314796666 94-95 19.349509662073466 27.0141948043618 25.233711668862647 28.402583864702084 96-97 19.930032266435564 27.243251712471295 24.638189945994114 28.18852607509903 98-99 20.151001042906035 27.555487007741007 24.408163065458265 27.88534888389469 100-101 20.096113828542332 27.826887390629512 24.455656908395635 27.621341872432513 102-103 19.97997424560682 28.643879414601663 24.663993301899264 26.71215303789225 104-105 19.908243853953987 28.67790850825267 25.06764200414225 26.346205633651103 106-107 19.099085865930267 29.343997414768136 25.478488226912848 26.078428492388745 108-109 18.99033965442109 29.91705714439597 25.41116448048101 25.681438720701934 110-111 18.6132777116781 30.042793421368312 26.012230888624487 25.3316979783291 112-113 18.225288984523566 30.131103107402136 25.94217023244818 25.70143767562612 114-115 18.246422604227405 29.940959912668337 26.493693612401014 25.318923870703237 116-117 18.25559739002596 29.918097500360425 26.911458798168347 24.914846311445263 118-119 17.849779635679358 30.351832757478636 27.229959937771408 24.568427669070598 120-121 17.80835350958997 30.76097093148114 27.425686164334262 24.004989394594624 122-123 17.298640936635877 31.583367653115257 27.27329332627326 23.844698083975604 124-125 16.63244121938561 32.51451766111421 27.782249772319982 23.070791347180194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27.0 1 21.5 2 20.5 3 34.0 4 52.0 5 108.5 6 255.0 7 434.0 8 716.5 9 993.5 10 1182.5 11 1317.5 12 1488.5 13 1781.0 14 2037.5 15 2324.0 16 2717.0 17 3217.0 18 3689.5 19 4274.0 20 4987.0 21 5683.0 22 6616.5 23 7714.0 24 8943.5 25 10528.0 26 12469.5 27 14836.0 28 17344.0 29 19901.5 30 22473.0 31 24394.0 32 25743.0 33 26199.5 34 25683.0 35 24358.0 36 22427.5 37 20746.5 38 19339.5 39 17782.5 40 16447.5 41 15672.5 42 15454.5 43 16382.5 44 17806.0 45 19708.5 46 22857.5 47 26777.5 48 36458.5 49 46348.0 50 58002.0 51 61388.0 52 47684.0 53 38499.0 54 36358.0 55 33426.5 56 28527.5 57 23345.0 58 19434.0 59 16415.5 60 13157.5 61 10480.5 62 8498.0 63 6243.5 64 4276.0 65 3298.0 66 2568.0 67 1920.0 68 1513.5 69 1224.0 70 1070.0 71 945.5 72 823.0 73 843.5 74 710.0 75 526.0 76 399.0 77 267.0 78 197.0 79 137.0 80 87.5 81 57.0 82 35.5 83 25.0 84 18.0 85 7.0 86 8.5 87 7.0 88 2.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12074207905521527 2 0.022522853351743317 3 0.0 4 1.9585089871081146E-4 5 0.01831205902946087 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.8962724677702866E-5 18-19 4.8962724677702866E-5 20-21 5.385899714547316E-4 22-23 9.792544935540573E-5 24-25 0.0011751053922648689 26-27 0.0012240681169425715 28-29 0.0012240681169425715 30-31 1.468881740331086E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.4066452209932575E-4 40-41 5.875526961324344E-4 42-43 0.32893158438480785 44-45 0.31566268599715036 46-47 0.3143896551555301 48-49 0.3231539828728389 50-51 0.3259938209041457 52-53 0.3296660252549734 54-55 0.3314776460680484 56-57 0.3333871923304788 58-59 0.3330934159824126 60-61 0.34841874880653356 62-63 0.3419556691490768 64-65 0.3491531896766991 66-67 0.36193246081757957 68-69 0.3723125584492526 70-71 0.35855403281481807 72-73 0.36080631814999237 74-75 0.37045197491149984 76-77 0.3676121368801931 78-79 0.44193755294094605 80-81 0.6061585315099615 82-83 0.5758506049344634 84-85 0.5395692259482856 86-87 0.4911450912420374 88-89 0.3245739018884923 90-91 0.01811620813075006 92-93 0.07765488133883675 94-95 1.468881740331086E-4 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.1759230697669864 114-115 0.1589819670285012 116-117 0.15256785009572213 118-119 0.1367528900248241 120-121 0.13924998898338695 122-123 0.1336682383701288 124-125 0.001468881740331086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1021185.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.10714081593591 #Duplication Level Percentage of deduplicated Percentage of total 1 76.66280081781896 34.58039751833407 2 13.750920117898612 12.405293802134773 3 4.261380223826556 5.766560334791669 4 1.7046463220881178 3.0756688676718387 5 0.853551328672256 1.925062998802432 6 0.4881621592445888 1.3211759554834197 7 0.33518067880099045 1.058332945422008 8 0.24447397292340517 0.8822017537989871 9 0.19599413402192215 0.7956661502181831 >10 1.2427604597998847 10.883921689682923 >50 0.14268869974811713 4.473688937135304 >100 0.09724560885312178 8.958402352253737 >500 0.013170962806637366 4.135264191280105 >1k 0.005926933262986815 5.252873724287277 >5k 8.780641871091577E-4 2.881406698902164 >10k+ 2.1951604677728943E-4 1.6040820798012045 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16200 1.586392279557573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8729 0.8547912474233366 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7745 0.7584326052576174 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6724 0.6584507214657481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5902 0.5779560020956046 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 3754 0.3676121368801931 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 3693 0.36163868446951336 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3661 0.3585050700901404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3633 0.355763157508189 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3302 0.3233498337715497 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 2946 0.28848837380102527 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2540 0.24873064136273054 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2280 0.22327002453032507 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 2051 0.20084509662793715 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1970 0.19291313523014927 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1970 0.19291313523014927 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1813 0.1775388396813506 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 1736 0.16999858008098437 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1715 0.1679421456445208 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 1504 0.14727987583053023 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 0.14081679617307344 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 1426 0.13964169078080857 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1406 0.13768318179370045 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 1354 0.13259105842721935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1248 0.12221096079554634 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1213 0.11878357006810715 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.11613958293551119 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 1070 0.10478023081028412 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 1050 0.10282172182317602 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1050 0.10282172182317602 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 1035 0.10135284008284492 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9585089871081146E-4 1.9585089871081146E-4 10-11 0.0 0.0 0.0 0.001419919015653383 1.9585089871081146E-4 12-13 0.0 0.0 0.0 0.002839838031306766 1.9585089871081146E-4 14-15 0.0 0.0 0.0 0.003623241626150012 2.448136233885143E-4 16-17 0.0 0.0 0.0 0.0068058187302006985 2.9377634806621717E-4 18-19 0.0 0.0 0.0 0.011653128473293282 2.9377634806621717E-4 20-21 0.0 0.0 0.0 0.01914442534898182 2.9377634806621717E-4 22-23 0.0 0.0 0.0 0.047787619285437996 2.9377634806621717E-4 24-25 0.0 0.0 0.0 0.1180980919226193 2.9377634806621717E-4 26-27 0.0 0.0 0.0 0.19727081772646485 2.9377634806621717E-4 28-29 0.0 0.0 0.0 0.36065942997595934 2.9377634806621717E-4 30-31 0.0 0.0 0.0 0.6464058911950332 2.9377634806621717E-4 32-33 0.0 0.0 0.0 1.0773268310834963 2.9377634806621717E-4 34-35 0.0 0.0 0.0 1.450618644026303 2.9377634806621717E-4 36-37 0.0 0.0 0.0 1.9195346582646633 2.9377634806621717E-4 38-39 0.0 0.0 0.0 2.4977844367083337 2.9377634806621717E-4 40-41 0.0 0.0 0.0 3.371426333132586 2.9377634806621717E-4 42-43 0.0 0.0 0.0 4.119380915309176 3.4273907274392005E-4 44-45 0.0 0.0 0.0 4.736702948045653 3.917017974216229E-4 46-47 0.0 0.0 0.0 5.335810847202025 3.917017974216229E-4 48-49 0.0 0.0 0.0 5.866909521781068 4.406645220993258E-4 50-51 0.0 0.0 0.0 6.3828787144347015 4.896272467770287E-4 52-53 0.0 0.0 0.0 6.998095350010037 4.896272467770287E-4 54-55 0.0 0.0 0.0 7.555976634987784 4.896272467770287E-4 56-57 0.0 0.0 0.0 8.07806616822613 5.875526961324343E-4 58-59 0.0 0.0 0.0 8.622384778468152 6.854781454878401E-4 60-61 0.0 0.0 0.0 9.122587973775564 6.854781454878401E-4 62-63 0.0 0.0 0.0 9.627981217898814 6.854781454878401E-4 64-65 0.0 0.0 0.0 10.145027590495356 6.854781454878401E-4 66-67 0.0 0.0 0.0 10.659136199611236 6.854781454878401E-4 68-69 0.0 0.0 0.0 11.225683886856935 6.854781454878401E-4 70-71 0.0 4.8962724677702866E-5 0.0 11.865381884771123 6.854781454878401E-4 72-73 0.0 9.792544935540573E-5 0.0 12.413127885740586 6.854781454878401E-4 74-75 0.0 9.792544935540573E-5 0.0 12.934042313586666 6.854781454878401E-4 76-77 0.0 9.792544935540573E-5 0.0 13.464406547295543 6.854781454878401E-4 78-79 0.0 9.792544935540573E-5 0.0 13.981403957167409 6.854781454878401E-4 80-81 0.0 9.792544935540573E-5 0.0 14.49933165880815 6.854781454878401E-4 82-83 0.0 9.792544935540573E-5 0.0 15.106077743014243 6.854781454878401E-4 84-85 0.0 9.792544935540573E-5 0.0 15.620627016652222 6.854781454878401E-4 86-87 0.0 9.792544935540573E-5 0.0 16.13072068234453 7.34440870165543E-4 88-89 0.0 9.792544935540573E-5 0.0 16.643654186068147 7.834035948432458E-4 90-91 0.0 9.792544935540573E-5 0.0 17.125006732374644 7.834035948432458E-4 92-93 0.0 9.792544935540573E-5 0.0 17.61296924651263 7.834035948432458E-4 94-95 0.0 9.792544935540573E-5 0.0 18.114543398111017 7.834035948432458E-4 96-97 0.0 9.792544935540573E-5 0.0 18.610584761820824 7.834035948432458E-4 98-99 0.0 9.792544935540573E-5 0.0 19.127337358069305 7.834035948432458E-4 100-101 0.0 9.792544935540573E-5 0.0 19.70857386271831 7.834035948432458E-4 102-103 0.0 9.792544935540573E-5 0.0 20.209658387069922 7.834035948432458E-4 104-105 0.0 9.792544935540573E-5 0.0 20.6838134128488 7.834035948432458E-4 106-107 0.0 9.792544935540573E-5 0.0 21.16124894118108 7.834035948432458E-4 108-109 0.0 9.792544935540573E-5 0.0 21.630115992694762 7.834035948432458E-4 110-111 0.0 9.792544935540573E-5 0.0 22.095604616205684 7.834035948432458E-4 112-113 0.0 9.792544935540573E-5 0.0 22.59918623951586 7.834035948432458E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 370 0.0 75.5627 1 GTATAAC 230 0.0 59.485523 1 TAGTACT 525 0.0 56.54468 5 ATAGTAC 515 0.0 55.336933 4 AGATTAC 585 0.0 53.811024 2 AGTGTAC 410 0.0 49.235245 3 GATTACT 680 0.0 45.402054 3 GCATAGT 615 0.0 45.39144 2 TAACGCA 325 0.0 43.843876 4 CATAGTA 650 0.0 43.843876 3 GACCGTT 190 0.0 43.7413 7 ACGCATA 140 4.0017767E-11 42.489662 1 TAAACGC 340 0.0 40.163353 4 AAACGCA 475 0.0 39.99792 5 TACAACG 255 0.0 39.596786 2 ATAACGC 335 0.0 38.99051 3 GTGTACT 525 0.0 38.450382 4 TTAGGAC 265 0.0 38.087646 3 GGTTTCA 565 0.0 36.849438 1 ATAAACG 400 0.0 35.62315 3 >>END_MODULE