FastQCFastQC Report
Fri 10 Feb 2017
SRR3557317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085230
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471031.1530072970187726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184060.45054990783872634No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140490.3438974060212032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128200.3138134205418055No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125970.30835473155734194No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT91670.22439373058554843No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT81260.1989116891827388No Hit
GTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTG76790.1879698327878724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.17937790528317868No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG71260.1744332632434404No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG70660.1729645576870825No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG68670.16809335092516212No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66030.16163104647718732No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65740.16092117212494766No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT64500.15788584730847469No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT61600.15078710378607812No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGG61440.15039544897104937No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60190.14733564572863708No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT60180.14731116730269778No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT59900.1466257713763974No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC59530.14572006961664338No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT57790.14146082350320546No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA57510.14077542757690512No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54540.13350533507293347No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG54340.1330157665541475No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51180.12528058395732922No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50400.12337126673406394No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG50270.12305304719685305No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA47610.11654178589699969No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT45610.11164610070914No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC45060.11029978728247858No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA44900.1099081324674498No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC43620.1067748939472196No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT43530.10655458811376593No Hit
GTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGG40870.10004332681391255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7200.072.678381
TACAACG5550.063.1382872
AGATTAC11750.056.1072352
GTACAAC6700.054.138991
AGTGTAC11100.054.041433
ATAGTAC10150.052.6629144
TAACGCA10600.052.1081354
TAGTACT10600.050.987535
GACCGTT5800.050.1680767
GCATAGT11050.048.3742262
TCACGCA6350.047.7007184
TTAGGAC6350.047.7007183
ATAACGC12400.045.501993
GTATCAC8250.043.967421
GATTACT15800.042.1007083
CATGGGT38850.040.3590854
AAACGCA9000.040.2546235
GTATAAC14700.038.8337171
CATAGTA13800.038.733963
ACAACGC9550.038.55823
Produced by FastQC (version 0.11.5)