##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557315.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2560841 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.610697813726038 33.0 32.0 34.0 27.0 34.0 2 31.870730748219042 33.0 32.0 34.0 27.0 34.0 3 32.0239874322537 33.0 32.0 34.0 27.0 34.0 4 31.837288218987435 33.0 32.0 34.0 27.0 34.0 5 32.02466260107519 33.0 32.0 34.0 28.0 34.0 6 35.361261007614296 38.0 36.0 38.0 29.0 38.0 7 35.74291414422059 38.0 36.0 38.0 31.0 38.0 8 35.962037861780566 38.0 37.0 38.0 31.0 38.0 9 36.06338698888373 38.0 37.0 38.0 32.0 38.0 10-11 36.03242665202565 38.0 37.0 38.0 31.5 38.0 12-13 36.024983784623885 38.0 37.0 38.0 31.0 38.0 14-15 36.034760260398826 38.0 37.0 38.0 31.0 38.0 16-17 35.98835031147971 38.0 37.0 38.0 31.0 38.0 18-19 35.97171749437001 38.0 37.0 38.0 31.0 38.0 20-21 35.939168031127274 38.0 37.0 38.0 31.0 38.0 22-23 35.871018934795245 38.0 37.0 38.0 31.0 38.0 24-25 35.755777691781724 38.0 37.0 38.0 30.0 38.0 26-27 35.62197770185654 38.0 37.0 38.0 29.0 38.0 28-29 35.49936485709187 38.0 37.0 38.0 29.0 38.0 30-31 35.353224194707906 38.0 36.0 38.0 28.0 38.0 32-33 35.181102223839744 38.0 36.0 38.0 27.0 38.0 34-35 34.89838162541134 38.0 36.0 38.0 26.0 38.0 36-37 34.64838894722476 38.0 35.5 38.0 24.5 38.0 38-39 34.38057497517417 38.0 35.0 38.0 20.0 38.0 40-41 33.99045665076434 38.0 34.5 38.0 16.0 38.0 42-43 33.406478379563595 38.0 34.0 38.0 15.0 38.0 44-45 32.888277132395174 38.0 32.0 38.0 15.0 38.0 46-47 32.128275047142715 38.0 28.0 38.0 15.0 38.0 48-49 31.447815971393773 38.0 25.5 38.0 15.0 38.0 50-51 30.904841612579617 38.0 24.0 38.0 15.0 38.0 52-53 30.482952670626563 37.0 20.0 38.0 15.0 38.0 54-55 30.18570578962146 37.0 16.0 38.0 15.0 38.0 56-57 29.89628036258401 37.0 15.0 38.0 15.0 38.0 58-59 29.684246112898066 37.0 15.0 38.0 15.0 38.0 60-61 29.47795392216854 36.5 15.0 38.0 15.0 38.0 62-63 29.3098983888496 36.0 15.0 38.0 15.0 38.0 64-65 29.15928400084191 36.0 15.0 38.0 14.5 38.0 66-67 28.94988443249698 36.0 15.0 38.0 14.0 38.0 68-69 28.788973817585706 35.0 15.0 38.0 14.0 38.0 70-71 28.559258267108344 35.0 15.0 38.0 14.0 38.0 72-73 28.332177788468712 34.5 15.0 38.0 14.0 38.0 74-75 28.138759883959995 34.0 14.5 38.0 14.0 38.0 76-77 27.993872520785164 34.0 14.0 38.0 14.0 38.0 78-79 27.772524924429124 34.0 14.0 38.0 14.0 38.0 80-81 27.671260925609985 34.0 14.0 38.0 14.0 38.0 82-83 27.531233489310736 34.0 14.0 38.0 13.5 38.0 84-85 27.39797511833027 34.0 14.0 38.0 13.0 38.0 86-87 27.2881008621777 34.0 14.0 38.0 13.0 38.0 88-89 27.176540831703335 33.0 14.0 38.0 13.0 38.0 90-91 27.122494524259803 33.0 14.0 38.0 13.0 38.0 92-93 27.050709317759285 33.0 14.0 38.0 13.0 38.0 94-95 26.958468526550455 33.0 14.0 38.0 13.0 38.0 96-97 26.87684983175449 32.5 14.0 38.0 13.0 38.0 98-99 26.770779208861462 31.0 14.0 38.0 13.0 38.0 100-101 26.693315984865908 31.0 14.0 38.0 13.0 38.0 102-103 26.60578341255861 31.0 14.0 38.0 13.0 38.0 104-105 26.435411843218695 29.0 14.0 38.0 13.0 38.0 106-107 26.275953876089922 28.0 14.0 38.0 13.0 38.0 108-109 26.123220262405983 26.5 14.0 38.0 13.0 38.0 110-111 25.98288823866847 25.5 14.0 38.0 13.0 38.0 112-113 25.67591603695817 24.0 14.0 38.0 13.0 38.0 114-115 25.558098296614276 24.0 14.0 38.0 13.0 38.0 116-117 25.453337399705802 24.0 14.0 38.0 13.0 38.0 118-119 25.280420182276057 23.0 14.0 38.0 13.0 38.0 120-121 25.18306974154194 23.0 14.0 38.0 13.0 38.0 122-123 25.049010071300795 23.0 14.0 38.0 13.0 38.0 124-125 24.28739172014194 22.0 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.39139438734578746 1101 2 -0.669009020331675 1101 3 -0.2527414931457663 1101 4 -0.34789071051974574 1101 5 -0.12090687577416048 1101 6 0.0938282813549165 1101 7 0.0806408095479938 1101 8 0.0023405827959450676 1101 9 0.05929115902052473 1101 10-11 0.10523782700864359 1101 12-13 0.19771362869570197 1101 14-15 0.013674040044847402 1101 16-17 0.04363800497796433 1101 18-19 -0.01511165056488295 1101 20-21 -0.0034697704008195274 1101 22-23 0.06838831957293934 1101 24-25 0.0340246077837989 1101 26-27 -0.08231799980107013 1101 28-29 -0.014162338247700745 1101 30-31 -0.11706338528699689 1101 32-33 -0.07216075772738151 1101 34-35 -0.08587511867492736 1101 36-37 -0.1292180844568307 1101 38-39 -0.18563154132137782 1101 40-41 -0.1635480458850438 1101 42-43 -0.0033745009035826 1101 44-45 0.041445949494367085 1101 46-47 -0.13472177001830588 1101 48-49 -0.18398227340446738 1101 50-51 -0.08420010228033448 1101 52-53 -0.22316347588640895 1101 54-55 -0.17623393907085116 1101 56-57 -0.16783499695347714 1101 58-59 -0.2991147844679709 1101 60-61 -0.16460360165239862 1101 62-63 -0.3168723458035494 1101 64-65 -0.12764617554659807 1101 66-67 -0.28814055557114315 1101 68-69 -0.5033809173086539 1101 70-71 -0.6212574188095132 1101 72-73 -0.6098331517595845 1101 74-75 -0.616483267450068 1101 76-77 -0.7647824936598795 1101 78-79 -0.6136203179090778 1101 80-81 -0.6614118804976563 1101 82-83 -0.7019111136746687 1101 84-85 -0.7216434544050081 1101 86-87 -0.6509228327438557 1101 88-89 -0.504935887481583 1101 90-91 -0.75242657356441 1101 92-93 -0.2718725541400602 1101 94-95 -0.2825480747695721 1101 96-97 -0.5763195873022404 1101 98-99 -0.46496373829655013 1101 100-101 -0.4791879957891396 1101 102-103 -0.5277690228599425 1101 104-105 -0.5544473325062995 1101 106-107 -0.4868906779108606 1101 108-109 -0.5976306992893576 1101 110-111 -0.8468649329704618 1101 112-113 -0.8179453382507553 1101 114-115 -0.8668769859565764 1101 116-117 -0.6368034534865004 1101 118-119 -0.5880047139339872 1101 120-121 -0.3757744366219953 1101 122-123 -0.4947882288898988 1101 124-125 -0.5109427582575563 1102 1 -0.7727411339755683 1102 2 -0.6837124804434893 1102 3 -0.4917597071327009 1102 4 -0.6585237718523338 1102 5 -0.2724197516275737 1102 6 -0.23882717785973995 1102 7 -0.16646683576063026 1102 8 -0.1984773719567059 1102 9 -0.3326377332861057 1102 10-11 -0.12119891316780951 1102 12-13 -0.0897052479457301 1102 14-15 -0.09841775232141003 1102 16-17 -0.19373383601099192 1102 18-19 -0.07410171825717526 1102 20-21 -0.023461299477553155 1102 22-23 -0.1668304950885684 1102 24-25 -0.18745574112611507 1102 26-27 -0.09735438922523088 1102 28-29 -0.09428515662199288 1102 30-31 -0.16747489092351486 1102 32-33 -0.014817563678654722 1102 34-35 -0.08593169162575265 1102 36-37 0.16201471023192227 1102 38-39 0.09736338063048322 1102 40-41 -0.029710462321041575 1102 42-43 -0.04710939705216077 1102 44-45 0.034956831777073205 1102 46-47 -0.0797311095616493 1102 48-49 -0.06430269659016474 1102 50-51 -0.0034196859334549856 1102 52-53 0.022876042071999336 1102 54-55 0.11305860366300635 1102 56-57 0.0906125211210771 1102 58-59 0.005408648498839597 1102 60-61 0.09011986425689145 1102 62-63 0.1214578810239395 1102 64-65 0.1450970268128451 1102 66-67 -9.634907460558395E-4 1102 68-69 0.06239414964018053 1102 70-71 0.09817178514899183 1102 72-73 -0.056278593156260825 1102 74-75 -0.022465531579658915 1102 76-77 -0.20356680603068256 1102 78-79 -0.1327740166035447 1102 80-81 -0.16758809472590386 1102 82-83 -0.09392711831264933 1102 84-85 -0.15181916598509204 1102 86-87 -0.11076730218720243 1102 88-89 -0.01710407836372596 1102 90-91 -0.06385941621500635 1102 92-93 0.062009737074458116 1102 94-95 0.1583684871370039 1102 96-97 0.13933249605925369 1102 98-99 0.08945443393654884 1102 100-101 0.04747889116158177 1102 102-103 0.11415949396250014 1102 104-105 0.0539542012715053 1102 106-107 0.1465819350235158 1102 108-109 0.09017272322396863 1102 110-111 -9.59638092716375E-4 1102 112-113 -0.2212922040341887 1102 114-115 -0.27113202282661675 1102 116-117 -0.1504142546145566 1102 118-119 -0.19291249251446985 1102 120-121 -0.030416347365900265 1102 122-123 -0.29713260195897107 1102 124-125 -0.4900305419546349 1103 1 -0.7379748754918509 1103 2 -0.7117518826267002 1103 3 -0.34893754579958625 1103 4 -0.15562572830869215 1103 5 -0.180478880318109 1103 6 -0.07340588137789439 1103 7 -0.00822270996847152 1103 8 -0.043490856950192835 1103 9 0.1326766542586313 1103 10-11 -0.007323625489583208 1103 12-13 0.09198732157359046 1103 14-15 -0.04797618680441218 1103 16-17 -0.03693408873446913 1103 18-19 0.020811977458684794 1103 20-21 -0.05197651322863095 1103 22-23 -0.04220778001865 1103 24-25 -0.11075533998135967 1103 26-27 -0.06284098455193288 1103 28-29 -0.08248371007662314 1103 30-31 -0.14896100661000133 1103 32-33 -0.07538126475461837 1103 34-35 -0.1275297277075964 1103 36-37 -0.09712720950850695 1103 38-39 -0.11593501912422255 1103 40-41 0.04033900383483768 1103 42-43 -0.008382603965110036 1103 44-45 0.18017735711996608 1103 46-47 -0.022377935024415763 1103 48-49 -0.14519033728489816 1103 50-51 -0.23562078161157984 1103 52-53 -0.24210456866770613 1103 54-55 -0.14555227419915084 1103 56-57 -0.040437682416463616 1103 58-59 -0.033850897139807046 1103 60-61 -0.13362227764297074 1103 62-63 -0.12108065067092255 1103 64-65 -0.03365530255400628 1103 66-67 -0.05067867178253849 1103 68-69 -0.05551075125969973 1103 70-71 -0.11880586563708562 1103 72-73 -0.3480974690190557 1103 74-75 -0.41226672527121977 1103 76-77 -0.2194251551103541 1103 78-79 -0.16584744163548848 1103 80-81 0.06379024854074089 1103 82-83 -0.10356382994884328 1103 84-85 -0.09492551088876766 1103 86-87 -0.13021643604885824 1103 88-89 -0.003485407977237287 1103 90-91 0.07956368022102467 1103 92-93 0.0028034145561655066 1103 94-95 -0.06008555619418843 1103 96-97 -0.052641708941973064 1103 98-99 -0.061004906290964556 1103 100-101 -0.2239434636568305 1103 102-103 -0.2705776179060848 1103 104-105 -0.16642239450159835 1103 106-107 -0.13153795026687476 1103 108-109 -0.17733406326151524 1103 110-111 -0.25743201137010985 1103 112-113 -0.27075192451993857 1103 114-115 -0.24647641395236164 1103 116-117 -0.26009578589201965 1103 118-119 -0.09478574498019654 1103 120-121 0.04675514062989805 1103 122-123 -0.062000878483285504 1103 124-125 -0.2069470158039799 1104 1 -0.11918226974023227 1104 2 -0.0770052921314317 1104 3 -0.164108174512549 1104 4 -0.3015884652206964 1104 5 -0.10456916746829847 1104 6 0.13331612462037867 1104 7 0.10552269417889448 1104 8 0.16572276891919557 1104 9 0.16578141768169985 1104 10-11 0.15634320842074345 1104 12-13 0.04428414704030814 1104 14-15 0.08747735904515253 1104 16-17 0.1335168006086178 1104 18-19 0.09346156384400217 1104 20-21 0.11566069701850523 1104 22-23 0.1945854614945901 1104 24-25 0.08735624229046124 1104 26-27 0.04451562597078862 1104 28-29 -0.005321196484729285 1104 30-31 -0.03521319183969496 1104 32-33 -0.0020819351327858726 1104 34-35 0.11529666019249163 1104 36-37 0.08432648612110683 1104 38-39 0.3053059920646035 1104 40-41 0.12222159749797612 1104 42-43 0.03900710923183226 1104 44-45 0.08770811104389509 1104 46-47 0.10151538816072758 1104 48-49 0.06447343224154523 1104 50-51 -0.06414835062048141 1104 52-53 -0.03404908641294213 1104 54-55 0.09575405522886271 1104 56-57 -0.00790190636140764 1104 58-59 0.2004951603185141 1104 60-61 0.10032924119411746 1104 62-63 0.14147323076949547 1104 64-65 0.19053986276490775 1104 66-67 0.009949861186264286 1104 68-69 0.10745081473238471 1104 70-71 0.06428729465423544 1104 72-73 0.012869049356805107 1104 74-75 0.1059437632527711 1104 76-77 -0.06287472118215476 1104 78-79 0.15730146511486964 1104 80-81 0.07728330867871236 1104 82-83 0.0697110238147296 1104 84-85 0.16934553762614257 1104 86-87 0.11827740673803078 1104 88-89 0.3033961330472259 1104 90-91 0.09479005860718814 1104 92-93 0.0508735662550599 1104 94-95 0.19106784652866082 1104 96-97 0.3211160568798306 1104 98-99 0.09601302777946685 1104 100-101 0.20428594343599116 1104 102-103 0.08622784385838145 1104 104-105 0.08842785920842999 1104 106-107 0.12248602225330885 1104 108-109 0.01624394080054614 1104 110-111 -0.007424136668504389 1104 112-113 -0.21686301116769258 1104 114-115 -0.195987121778753 1104 116-117 0.07358232009117671 1104 118-119 -0.009777778674919801 1104 120-121 0.050165609611770634 1104 122-123 -0.225988003828288 1104 124-125 -0.28607876727582493 1105 1 0.005283947123519539 1105 2 0.011475135716519702 1105 3 0.007427882530528507 1105 4 0.01776927122765315 1105 5 -0.12733235009151755 1105 6 0.23848400416181192 1105 7 -0.0550710419358964 1105 8 0.004022883547996514 1105 9 0.08589317142767072 1105 10-11 0.052976516363983706 1105 12-13 0.0013838361361351303 1105 14-15 -0.09641629878352376 1105 16-17 -0.010457470764173138 1105 18-19 -0.05642741673133145 1105 20-21 -0.03869387500285626 1105 22-23 -3.838463640093437E-4 1105 24-25 -0.18464873802599158 1105 26-27 0.039848562895080875 1105 28-29 0.10770314315505658 1105 30-31 0.011527448364027748 1105 32-33 0.10281633025092418 1105 34-35 0.043698621452683994 1105 36-37 0.0039046123073731565 1105 38-39 0.03946246535583953 1105 40-41 0.016495198149165446 1105 42-43 -0.08131532627546534 1105 44-45 0.008999446176936488 1105 46-47 -0.12721784224315869 1105 48-49 -0.1308704261564131 1105 50-51 -0.25221573829110255 1105 52-53 -0.17942004624556773 1105 54-55 -0.15180207056659967 1105 56-57 -0.15758451464579437 1105 58-59 -0.30733928569828706 1105 60-61 -0.2351177551205872 1105 62-63 -0.13106475480046598 1105 64-65 -0.1628892932758781 1105 66-67 -0.09278596796828253 1105 68-69 -0.18261333570142213 1105 70-71 -0.05603926363158962 1105 72-73 -0.10124860447417561 1105 74-75 -0.27382916966386617 1105 76-77 -0.4536690079134651 1105 78-79 -0.5120307723372584 1105 80-81 -0.5680153080590848 1105 82-83 -0.5609930347417915 1105 84-85 -0.47372623419159865 1105 86-87 -0.2959698890304132 1105 88-89 -0.18139431467718836 1105 90-91 -0.25916059002255665 1105 92-93 -0.338233181659902 1105 94-95 -0.26421027455469925 1105 96-97 -0.394329302769588 1105 98-99 -0.3525959156317455 1105 100-101 -0.3060542184631885 1105 102-103 -0.16723801269008476 1105 104-105 -0.40934444020050265 1105 106-107 -0.2970385856004434 1105 108-109 -0.13666128009948153 1105 110-111 -0.33316922387227166 1105 112-113 -0.3431478400894967 1105 114-115 -0.3275597642028636 1105 116-117 -0.46473898406346237 1105 118-119 -0.23483419073123457 1105 120-121 -0.2722506061940635 1105 122-123 -0.2412821119577231 1105 124-125 -0.29247879751990524 1106 1 0.370978031873733 1106 2 0.19514087343374698 1106 3 0.20312539219535353 1106 4 0.10813658159059969 1106 5 0.10680278859406656 1106 6 0.10251723990854344 1106 7 0.27116263107389926 1106 8 0.16833785370901921 1106 9 0.10504571618614733 1106 10-11 0.12034235823615091 1106 12-13 0.0050104922934721685 1106 14-15 0.13176863722218002 1106 16-17 0.05338434509739898 1106 18-19 0.07706918650404049 1106 20-21 0.10275216617118588 1106 22-23 0.10516979342880717 1106 24-25 0.09923445831075384 1106 26-27 0.03340749488472028 1106 28-29 0.04341151402169885 1106 30-31 -0.0950800564214731 1106 32-33 0.0195668604089434 1106 34-35 0.07086804681770076 1106 36-37 0.2721446284466964 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-0.04067656369782213 2315 24-25 -0.09724922679249914 2315 26-27 -0.04723501346507675 2315 28-29 0.060481335397192026 2315 30-31 0.01749743059480835 2315 32-33 0.04877394637254184 2315 34-35 -0.11965383752664849 2315 36-37 -0.2323248095374595 2315 38-39 -0.20561341553162293 2315 40-41 -0.13664222165198936 2315 42-43 0.08853838287907223 2315 44-45 -0.1810695734415404 2315 46-47 -0.06633877465231564 2315 48-49 -0.3167545803552372 2315 50-51 -0.1447648184063084 2315 52-53 -0.20794433084241604 2315 54-55 -0.2466636357045715 2315 56-57 -0.041472796909584275 2315 58-59 0.06513973486969249 2315 60-61 0.1774909287365567 2315 62-63 0.16287816893658302 2315 64-65 0.01688330143675998 2315 66-67 -0.0048563191476986844 2315 68-69 0.12798314255728727 2315 70-71 0.1242507521481997 2315 72-73 0.13627422874386141 2315 74-75 0.07710043620313911 2315 76-77 -0.06212453287401942 2315 78-79 0.2986527434357491 2315 80-81 0.16368448938088065 2315 82-83 0.16246278411166415 2315 84-85 0.28719301760227367 2315 86-87 0.09836769896790543 2315 88-89 0.1695299219925097 2315 90-91 0.09468872882411716 2315 92-93 0.17939756677229113 2315 94-95 0.032599517956906965 2315 96-97 -0.033992863214315605 2315 98-99 0.13118091339945082 2315 100-101 0.1870254513820413 2315 102-103 0.07429984975899373 2315 104-105 0.14225919774152018 2315 106-107 0.14999626105528208 2315 108-109 0.09236522225844368 2315 110-111 0.34026545729914304 2315 112-113 0.30559842407953397 2315 114-115 0.42300767517607696 2315 116-117 0.30121431409315136 2315 118-119 0.3423403906974478 2315 120-121 0.2142043219378209 2315 122-123 0.21923281101972947 2315 124-125 0.41680375338008346 2316 1 -0.29859663682447746 2316 2 -0.20939677364167864 2316 3 -0.13380870249723387 2316 4 0.21567887018290932 2316 5 0.12530595818108026 2316 6 -0.16101405153357717 2316 7 -0.51640655668416 2316 8 -0.4847754778373101 2316 9 -0.48326096487063097 2316 10-11 -0.45249480423105126 2316 12-13 -0.2813533471391949 2316 14-15 -0.15163929191826497 2316 16-17 -0.27109095657768023 2316 18-19 -0.1575531435656572 2316 20-21 -0.17907640690331306 2316 22-23 -0.24213879102611457 2316 24-25 -0.2403445838532079 2316 26-27 -0.1713560851967273 2316 28-29 -0.07279526273404002 2316 30-31 -0.006183780451074483 2316 32-33 0.043530543008195366 2316 34-35 -0.01250290772647844 2316 36-37 -0.071341435246012 2316 38-39 -0.14539649048327163 2316 40-41 -0.034685976021130216 2316 42-43 0.07396001352186232 2316 44-45 0.09889343962539243 2316 46-47 -0.02754441610849767 2316 48-49 -0.08803325528377215 2316 50-51 -0.19982940532985083 2316 52-53 -0.18012453747978086 2316 54-55 -0.026522553979820174 2316 56-57 -0.04643677303038274 2316 58-59 0.017227805578240662 2316 60-61 -0.04432784945401025 2316 62-63 -0.1424082036247647 2316 64-65 -0.11180661108395995 2316 66-67 -0.20014268037899896 2316 68-69 -0.24330400158015308 2316 70-71 -0.28362549883043187 2316 72-73 -0.09742155274035369 2316 74-75 -0.2531276149497508 2316 76-77 0.05904010514802849 2316 78-79 0.036762427338810966 2316 80-81 0.06040956809637166 2316 82-83 -0.20352643081816169 2316 84-85 -0.036566852283481666 2316 86-87 -0.29132708660532103 2316 88-89 -0.2752868088588656 2316 90-91 -0.18356982054485727 2316 92-93 -0.27863367922678606 2316 94-95 -0.47517006064471445 2316 96-97 -0.35300006232513326 2316 98-99 -0.48726408886089345 2316 100-101 -0.42170432023823423 2316 102-103 -0.4634786285932577 2316 104-105 -0.557473639789027 2316 106-107 -0.4362752362895215 2316 108-109 -0.44115698293094496 2316 110-111 -0.4309982053193018 2316 112-113 -0.5506417116997291 2316 114-115 -0.4695104160738417 2316 116-117 -0.6195939090271665 2316 118-119 -0.8343183845926312 2316 120-121 -0.6154167220352171 2316 122-123 -0.6746413450335176 2316 124-125 -0.7942885876225567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 14.0 10 17.0 11 39.0 12 90.0 13 247.0 14 612.0 15 3900.0 16 7932.0 17 10987.0 18 17554.0 19 29180.0 20 50577.0 21 90484.0 22 150253.0 23 218792.0 24 219841.0 25 136465.0 26 85872.0 27 64956.0 28 52628.0 29 50128.0 30 55320.0 31 63036.0 32 76652.0 33 100286.0 34 134792.0 35 173302.0 36 261028.0 37 505857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.17047371620816 15.131707298001567 10.366551917599613 20.33126706819066 2 14.58075354590196 24.98077728873274 36.95103002101319 23.487439144352116 3 21.420228653947007 36.47404164224408 24.716525572152282 17.389204131656626 4 13.281482167033545 18.550361977998204 33.55146905780284 34.61668679716541 5 13.127125729958436 40.17541262935002 28.415471272061804 18.281990368629742 6 27.27732803403257 33.15922386434769 21.561822854288884 18.001625247330857 7 25.6813679568548 35.781604558814855 21.282461503857522 17.254565980472822 8 30.222141866675827 26.068935947214218 22.902398079380955 20.806524106729 9 34.1246098449689 14.238564596552461 21.18511067262669 30.451714885851956 10-11 23.422246832193018 27.564889815494208 25.60604114039099 23.40682221192179 12-13 26.886460346425256 26.211877270006223 29.39448407769166 17.50717830587686 14-15 26.004035393060327 23.03538173592191 31.9613361391824 18.999246731835363 16-17 18.56616634925792 27.665833216509732 31.652140839669467 22.11585959456288 18-19 19.042654119120854 25.25752010245158 38.15958133714618 17.540244441281395 20-21 21.531683984304298 25.026529500120766 36.69105525809323 16.75073125748171 22-23 19.552131069569594 28.029803519433184 34.00196849586571 18.416096915131508 24-25 18.35926959590039 29.438943404655287 34.876616127043235 17.325170872401085 26-27 19.366706211569657 28.60295329353851 34.93473919498657 17.095601299905265 28-29 18.69487406676166 29.533344709804314 35.488536773661465 16.283244449772553 30-31 19.899231102658923 29.884860649560103 34.23123933442934 15.984668913351635 32-33 16.69094254582772 30.788420678987876 34.87674556913139 17.643891206053013 34-35 15.764625761615031 32.26196003578512 34.16463575833095 17.808778444268896 36-37 17.369001043016727 32.835775434710705 32.501334522526 17.293888999746567 38-39 17.23494352050752 31.931795062637626 32.579687688536694 18.25357372831816 40-41 16.193414127245333 32.95093944986516 32.30765235551716 18.547994067372343 42-43 17.21608099824003 32.49417666558524 31.969748617172222 18.319993719002504 44-45 17.44921008253325 31.565943004720825 32.0171957646062 18.967651148139723 46-47 16.273776869975233 31.034492210896232 32.873379754223684 19.81835116490485 48-49 16.17813900901864 31.000446839598684 32.529746554616054 20.29166759676662 50-51 15.208811109960084 30.058319183214195 32.52702948544742 22.205840221378295 52-53 15.78964785636589 28.626368623411818 32.18638048979168 23.397603030430606 54-55 16.450019172402396 27.62222259648771 32.252230480868924 23.675527750240978 56-57 17.160678636851266 26.001213390219323 32.52473291119261 24.313375061736807 58-59 16.558945636847877 25.88569246435845 33.368106689644364 24.187255209149303 60-61 17.209387853897088 25.414144681957723 32.963472705382316 24.412994758762878 62-63 16.200275140875064 25.679945559314397 33.91979790364195 24.19998139616859 64-65 15.877149001343554 25.542396383762973 33.77955917318532 24.800895441708153 66-67 16.436553584538157 25.2064710587944 33.60845832069944 24.748517035968 68-69 16.92136712259488 24.754627885836374 33.83000265013048 24.494002341438268 70-71 16.569655952134244 24.85762773349463 33.52201388445448 25.050702429916647 72-73 17.437345853552376 24.434642102089878 33.04821484432617 25.079797200031578 74-75 17.355752834384056 23.982952852562665 32.721751117210694 25.939543195842585 76-77 17.00764806183481 24.40721889283801 31.656472841986087 26.928660203341092 78-79 17.572173290329506 24.268061979730014 30.727803087077266 27.431961642863218 80-81 17.387911558947483 24.30075517420362 29.424113905115192 28.887219361733706 82-83 17.50813133693819 24.10094122162961 28.095451906875535 30.295475534556658 84-85 18.164263832629178 23.672496487079002 27.277355756383585 30.88588392390823 86-87 18.465737303801628 23.231868352791764 26.531933568370935 31.770460775035676 88-89 18.95464516307182 23.442696759479123 25.73606407993973 31.866593997509323 90-91 19.72352224528384 23.918178706256885 24.995806534697973 31.362492513761303 92-93 19.834301011621324 24.56097838850738 23.999209728855313 31.605510871015984 94-95 20.03721043930694 24.77862248742161 24.35948275575929 30.824684317512162 96-97 20.50334245663827 25.183289395944534 23.87887416672882 30.434493980688377 98-99 21.14278082864184 25.695913959515643 23.651409829817627 29.509895382024894 100-101 21.43344705899351 25.85131212753935 23.759499320730963 28.955741492736177 102-103 21.367277390513507 26.876385531159492 24.027907238286172 27.728429840040832 104-105 21.33656482382155 26.881540087807092 24.713268024059285 27.06862706431207 106-107 20.636540886372874 27.393383657946746 25.44257921518751 26.52749624049287 108-109 20.330274312227896 27.820567540116702 25.948389611069178 25.90076853658622 110-111 20.101013690424356 27.913701006817682 26.709467710021823 25.27581759273614 112-113 19.165699283209957 28.265222492976523 27.181055044183918 25.388023179629606 114-115 18.967527331200404 27.99330422518182 27.903405074616295 25.13576336900148 116-117 18.599648576012946 28.469803242475116 28.474498285722333 24.456049895789604 118-119 18.077263251859957 29.37770915635683 28.745564523542498 23.799463068240716 120-121 17.777009728472404 30.11846019545355 28.85898321355872 23.24554686251533 122-123 17.278579567256635 31.09414438781916 28.641173164231965 22.98610288069224 124-125 16.2789112976523 32.60046504480874 29.273006763240883 21.847616894298074 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 28.0 1 16.0 2 15.5 3 47.0 4 77.5 5 200.0 6 515.0 7 961.5 8 1657.5 9 2438.0 10 2983.0 11 3574.0 12 4246.0 13 5058.0 14 6041.5 15 7070.5 16 8326.5 17 9630.0 18 11136.0 19 12975.0 20 15082.0 21 17340.5 22 19927.0 23 23058.5 24 26794.0 25 31174.5 26 36247.0 27 42246.5 28 48536.0 29 55485.5 30 62923.5 31 69424.5 32 74415.5 33 76757.5 34 75836.5 35 72233.5 36 66883.0 37 60867.0 38 54443.0 39 48010.5 40 42462.5 41 38005.5 42 34731.5 43 34643.5 44 36960.0 45 39755.5 46 46301.5 47 53535.5 48 73218.5 49 97624.0 50 125666.0 51 143101.5 52 135136.5 53 120011.5 54 101431.5 55 85869.0 56 70368.0 57 54660.0 58 42042.0 59 32707.0 60 24405.0 61 18898.5 62 14864.5 63 10115.0 64 6602.0 65 4930.5 66 3732.5 67 2687.5 68 2055.5 69 1653.0 70 1415.5 71 1250.0 72 1060.0 73 1138.5 74 916.5 75 649.0 76 540.0 77 348.5 78 234.0 79 150.5 80 102.5 81 67.5 82 45.0 83 31.5 84 17.5 85 14.0 86 8.5 87 3.5 88 2.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12050728647346712 2 0.002655377666946132 3 0.0010933908040366427 4 0.001952483578636862 5 0.00976241789318431 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.395679388138505E-4 20-21 4.881208946592155E-4 22-23 9.76241789318431E-5 24-25 4.685960588728468E-4 26-27 0.0015229371913367522 28-29 0.0 30-31 0.0012105398187548543 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0033973214268281397 42-43 0.2775064910316572 44-45 0.2670997535575227 46-47 0.28027901771332153 48-49 0.2870931854027642 50-51 0.2953912406119708 52-53 0.28929949184662385 54-55 0.3003700737374948 56-57 0.2992766829334582 58-59 0.29837854048728524 60-61 0.29783184508526694 62-63 0.2969141778033076 64-65 0.30892195181192433 66-67 0.31954346247970883 68-69 0.3177276527515765 70-71 0.3202854062395908 72-73 0.32319460677175976 74-75 0.3276462693310518 76-77 0.32151547089413207 78-79 0.3202073068964454 80-81 0.5419703917580202 82-83 0.5069428363572748 84-85 0.4839035301293599 86-87 0.45311286409425655 88-89 0.3013658403625996 90-91 0.012085873351762175 92-93 0.08690504408512671 94-95 1.952483578636862E-5 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.2280110323132127 114-115 0.20303876734244727 116-117 0.19345207297134026 118-119 0.17431773390069902 120-121 0.17890607031049566 122-123 0.16666399827244252 124-125 4.881208946592155E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2560841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.233626762934136 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30114943775466 23.94995519033577 2 13.00957989853922 8.386918855841676 3 5.268717483892804 5.094896186854382 4 2.315590850052874 2.9855956478507895 5 1.1681274787737146 1.8826492581159593 6 0.7193298345487777 1.391196564377308 7 0.4642104696395127 1.0474230912464495 8 0.33132684371301807 0.8543892653429148 9 0.2597496870672936 0.7535407018244477 >10 1.7164687833426542 10.967971929984762 >50 0.2164332801883482 4.887536395900162 >100 0.18503591030120295 11.922156721316037 >500 0.02250496649562824 4.96628108544172 >1k 0.019707051742117704 11.80209822286555 >5k 0.001338133142983301 3.134858818486804 >10k+ 7.298908052636187E-4 5.972532064215262 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58711 2.292645267706976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23639 0.9230951863079355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22937 0.8956823168638739 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 17815 0.6956698990683139 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 15969 0.6235842053450409 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13244 0.517173850309332 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 9498 0.37089378059785827 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9250 0.36120946204781945 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 8834 0.3449647986735607 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8260 0.3225502871908096 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7583 0.2961136595360665 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7528 0.2939659275995659 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 7148 0.2791270524019258 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 5717 0.2232469723813388 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5446 0.212664511385127 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 5391 0.21051677944862643 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5292 0.20665086196292545 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4758 0.1857983373430838 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 4655 0.18177622117109185 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4606 0.1798627872640277 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 4393 0.17154520721903468 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4268 0.16666399827244252 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 4137 0.16154849129641397 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 4136 0.1615094416248412 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 3892 0.15198132176109333 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3792 0.1480763546038196 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3711 0.1449133312064279 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 3671 0.1433513443435184 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 3493 0.13640050280357116 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 3458 0.13503376429852537 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 3287 0.1283562704595873 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3285 0.12827817111644182 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 3226 0.12597424049365033 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 3158 0.12331886282670419 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 3010 0.11753951143393909 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 2997 0.1170318657034935 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2880 0.11246305412948325 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.11058866989399185 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 2807 0.10961242810467342 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 2706 0.10566841127582698 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 2599 0.10149009641754408 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 2583 0.10086530167238028 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.904967157273724E-5 0.0 4 0.0 0.0 0.0 7.809934314547448E-5 0.0 5 0.0 0.0 0.0 7.809934314547448E-5 0.0 6 0.0 0.0 0.0 7.809934314547448E-5 3.904967157273724E-5 7 0.0 0.0 0.0 1.952483578636862E-4 3.904967157273724E-5 8 0.0 0.0 0.0 2.3429802943642343E-4 3.904967157273724E-5 9 0.0 0.0 0.0 2.3429802943642343E-4 3.904967157273724E-5 10-11 7.809934314547448E-5 0.0 0.0 4.490712230864782E-4 3.904967157273724E-5 12-13 7.809934314547448E-5 0.0 0.0 7.028940883092702E-4 3.904967157273724E-5 14-15 7.809934314547448E-5 0.0 0.0 0.001073865968250274 3.904967157273724E-5 16-17 7.809934314547448E-5 0.0 0.0 0.0025382286522279206 3.904967157273724E-5 18-19 7.809934314547448E-5 0.0 0.0 0.0046273860813693625 3.904967157273724E-5 20-21 7.809934314547448E-5 0.0 0.0 0.010992482547725533 3.904967157273724E-5 22-23 7.809934314547448E-5 0.0 0.0 0.03567187498169547 3.904967157273724E-5 24-25 7.809934314547448E-5 0.0 0.0 0.09502737577225606 3.904967157273724E-5 26-27 7.809934314547448E-5 0.0 0.0 0.18380680409287417 3.904967157273724E-5 28-29 7.809934314547448E-5 0.0 0.0 0.3964517906422148 3.904967157273724E-5 30-31 7.809934314547448E-5 0.0 0.0 0.8813120377251067 3.904967157273724E-5 32-33 7.809934314547448E-5 0.0 0.0 1.6607239574811556 3.904967157273724E-5 34-35 7.809934314547448E-5 0.0 0.0 2.263416588534782 1.1714901471821172E-4 36-37 7.809934314547448E-5 0.0 0.0 2.9670721454397206 1.1714901471821172E-4 38-39 7.809934314547448E-5 0.0 0.0 3.7373659668835355 1.1714901471821172E-4 40-41 7.809934314547448E-5 0.0 0.0 4.9075869997395385 1.1714901471821172E-4 42-43 7.809934314547448E-5 0.0 0.0 5.908898678207667 1.1714901471821172E-4 44-45 7.809934314547448E-5 0.0 0.0 6.703637594056016 1.1714901471821172E-4 46-47 7.809934314547448E-5 0.0 0.0 7.475688650720603 1.1714901471821172E-4 48-49 7.809934314547448E-5 0.0 0.0 8.145644341058269 1.1714901471821172E-4 50-51 7.809934314547448E-5 0.0 0.0 8.785024138554483 1.1714901471821172E-4 52-53 7.809934314547448E-5 0.0 0.0 9.547683749205827 1.5619868629094895E-4 54-55 7.809934314547448E-5 0.0 0.0 10.215569806950139 1.952483578636862E-4 56-57 7.809934314547448E-5 0.0 0.0 10.822460277697834 2.3429802943642343E-4 58-59 7.809934314547448E-5 0.0 0.0 11.472110138817676 2.3429802943642343E-4 60-61 7.809934314547448E-5 0.0 0.0 12.044187827358279 2.3429802943642343E-4 62-63 7.809934314547448E-5 1.952483578636862E-5 0.0 12.606815495378276 2.3429802943642343E-4 64-65 7.809934314547448E-5 3.904967157273724E-5 0.0 13.197305104065421 2.3429802943642343E-4 66-67 7.809934314547448E-5 3.904967157273724E-5 0.0 13.753391952096987 2.538228652227921E-4 68-69 7.809934314547448E-5 3.904967157273724E-5 0.0 14.355869809956964 2.7334770100916067E-4 70-71 7.809934314547448E-5 3.904967157273724E-5 0.0 15.056186619942434 2.7334770100916067E-4 72-73 7.809934314547448E-5 3.904967157273724E-5 0.0 15.650424997100561 2.7334770100916067E-4 74-75 7.809934314547448E-5 3.904967157273724E-5 0.0 16.19751089583461 2.7334770100916067E-4 76-77 7.809934314547448E-5 3.904967157273724E-5 0.0 16.764141155190813 2.7334770100916067E-4 78-79 7.809934314547448E-5 3.904967157273724E-5 0.0 17.276980491955573 2.7334770100916067E-4 80-81 7.809934314547448E-5 3.904967157273724E-5 0.0 17.83773377574008 2.7334770100916067E-4 82-83 7.809934314547448E-5 5.857450735910586E-5 0.0 18.530494474276225 2.7334770100916067E-4 84-85 7.809934314547448E-5 7.809934314547448E-5 0.0 19.075413116237986 2.7334770100916067E-4 86-87 7.809934314547448E-5 7.809934314547448E-5 0.0 19.581574959163806 2.7334770100916067E-4 88-89 7.809934314547448E-5 7.809934314547448E-5 0.0 20.094629069122213 2.7334770100916067E-4 90-91 7.809934314547448E-5 7.809934314547448E-5 0.0 20.586791604789205 2.7334770100916067E-4 92-93 7.809934314547448E-5 7.809934314547448E-5 0.0 21.047323906482283 2.7334770100916067E-4 94-95 7.809934314547448E-5 7.809934314547448E-5 0.0 21.522773182716147 2.7334770100916067E-4 96-97 7.809934314547448E-5 7.809934314547448E-5 0.0 21.98326643473765 2.7334770100916067E-4 98-99 7.809934314547448E-5 7.809934314547448E-5 0.0 22.464065515976976 2.7334770100916067E-4 100-101 7.809934314547448E-5 7.809934314547448E-5 0.0 23.024974998447775 2.7334770100916067E-4 102-103 7.809934314547448E-5 7.809934314547448E-5 0.0 23.493180560604895 2.7334770100916067E-4 104-105 7.809934314547448E-5 7.809934314547448E-5 0.0 23.929345867236584 2.7334770100916067E-4 106-107 7.809934314547448E-5 7.809934314547448E-5 0.0 24.361137610652126 2.7334770100916067E-4 108-109 7.809934314547448E-5 7.809934314547448E-5 0.0 24.812668963047685 2.7334770100916067E-4 110-111 7.809934314547448E-5 7.809934314547448E-5 0.0 25.26191590965624 2.9287253679552926E-4 112-113 7.809934314547448E-5 7.809934314547448E-5 0.0 25.739024796931943 3.123973725818979E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 1225 0.0 82.52405 1 TACAACG 755 0.0 77.09552 2 GTACAAC 840 0.0 70.79269 1 ATAGTAC 1525 0.0 67.37664 4 GCATAGT 1540 0.0 66.337296 2 TAGTACT 1545 0.0 65.73561 5 AGATTAC 1755 0.0 63.963863 2 GTATAAC 795 0.0 63.579845 1 ATAACGC 860 0.0 61.46574 3 TCACGCA 605 0.0 60.865246 4 AGTGTAC 1280 0.0 59.39386 3 GGTTTCA 1470 0.0 56.634148 1 ACAACGC 1045 0.0 56.267868 3 GTGTACT 1375 0.0 53.993366 4 CATAGTA 1940 0.0 53.576935 3 GATTACT 2175 0.0 51.611214 3 TATCACG 680 0.0 51.533916 2 TAAACGC 850 0.0 49.61037 4 GTATCAC 880 0.0 47.302383 1 ATCACGC 795 0.0 44.07846 3 >>END_MODULE