FastQCFastQC Report
Fri 10 Feb 2017
SRR3557300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951614
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147351.5484219441916576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64330.6760093903620585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.6245179242844262No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.33437927563066533No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30990.3256572517848623No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25520.26817596210228095No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22610.23759633632964627No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA22130.23255227434653128No Hit
GTAATAGACCGCAGAGTCCTCAGATGTCAGGCTGCTGAGTTGCATGTAGG18280.192094693856963No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG15610.16403709907588582No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT14600.15342355198641466No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT14080.14795915150470673No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA13970.14680322063357623No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13850.14554220513779748No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.14249475102299883No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT13480.14165407402581298No Hit
TCCTAATAGTGGTAGTACTAACTACAATGAGAAGTTCAAGAGCAAGGCCA12980.13639984279340153No Hit
CTCTTAGATAGTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCC12910.1356642504208639No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12590.13230154243212058No Hit
CCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTCTG12230.12851849594478434No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA11760.12357951858631756No Hit
CTCCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTC11620.12210833384124235No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG11560.12147782609335298No Hit
GTCCTGCCCAGTTCCTGTTTCTGTTAGTGCTCTGGATTCGGGAAACCAAC11460.1204269798468707No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT11410.11990155672362954No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG11310.11885071047714724No Hit
GTCTATTACTGTGCAAGTAGTAACTACTACTTTGACTACTGGGGCCAAGG11160.11727444110742381No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT10690.11233546374895703No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC10630.11170495600106767No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.10876258651091725No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.10592530164541505No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9790.10287784753061642No Hit
ATCTAAGAGGCTCTGACTTGACTTGCAAGAGATGGAGGCTGGTTGTCCAA9610.10098632428694827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2750.080.0631
ATAGTAC3250.071.298744
GCATAGT3350.067.42872
ATAACGC3250.065.814223
CATAGTA3700.062.6272743
AGATTAC4400.062.1457182
TACAACG1700.059.443732
TAACGCA3400.059.415624
AGTGTAC4100.059.4156153
GATTACT5000.054.6623653
GTATAAC3850.054.0966261
TCACGCA1550.053.6657184
GTACAAC1900.053.2424661
GTATCAC2700.044.078731
TATCACG1900.043.8006362
TAGACCG5100.041.9404375
CAGTGTA6250.041.848382
TGTACTT6500.041.1338845
GCAGTGT7650.039.670861
AAACGCA2550.039.6104135
Produced by FastQC (version 0.11.5)