FastQCFastQC Report
Fri 10 Feb 2017
SRR3557297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5818111
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1181602.0308997198575276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439210.7549013760651868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400750.6887974464564186No Hit
GTACATGGGCAGGAAACAGCTATGACCACAACCCCAAAAAAAAAAAAAAA166140.2855566007592499No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149100.2562687442711217No Hit
GTACATGGGGAGGAGAAAACAGCTGAAGCGAGGAAAAAAAAAAAAAAAAA146490.2517827521681866No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT114620.1970055229265994No Hit
GCTATGACCACAACCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110510.18994137444266704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87390.15020339075689687No Hit
GGTCATAGCTGTTTCCTGCCCATGTACTCTGCGTTGATACCTGTCTCTTA86590.14882837402036503No Hit
ACGCAGAGTACATGGGCAGGAAACAGCTATGACCACAACCCCAAAAAAAA86170.1481064902336858No Hit
GTATCAACGCAGAGTACATGGGCAGGAAACAGCTATGACCACAACCCCAA85700.14729866790097335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG81160.13949544792115517No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.13574852731410592No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT76300.13114222124672423No Hit
GTATCAACGCAGAGTACATGGGCAGGAAACAGCTATGACCCTGTCTCTTA68010.11689361031441303No Hit
GCTATGACCACAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67600.11618891423694049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC65070.11184042380765853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131950.095.315911
GTATCAA470000.085.354241
TATCAAC664250.060.4819262
ATCAACG718600.055.7221833
TCAACGC740850.054.1369134
CAACGCA763850.052.4912575
AACGCAG784500.051.298936
GGTCATA55950.046.3613621
ACGCAGA925050.043.318397
CGCAGAG927300.043.2004628
GTCATAG60350.042.344952
GCAGAGT1003550.039.8588649
GGAACGC20550.039.328879
TCATAGC76300.036.4506843
CATGGGG303950.035.466594
TACATGG609650.035.1134572
GTACATG623200.034.491361
ACATGGG618150.034.4843333
AGGACGC5050.034.126445
GGTTCAC49500.033.9754756
Produced by FastQC (version 0.11.5)