FastQCFastQC Report
Fri 10 Feb 2017
SRR3557283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6704467
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT633150.9443703727678875No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA318840.4755635310010475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309040.460946410803424No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT256960.3832668577531965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240360.3585072459898751No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT222990.3325991462110262No Hit
ATGGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGG195510.29161154794258815No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC189310.28236398210327535No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG186280.2778446071850305No Hit
TCATTATGGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAA182190.2717441968168387No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA171040.2551134937348487No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC168840.25183209940476997No Hit
GACATGAGTGTGCCCACTCAGGTCCTGGGGTTGCTGCTGCTGTGGCTTAC166090.24773035649217154No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT157910.2355295357557879No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG143000.2132906314551179No Hit
TTATGGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAAC142380.21236587487118663No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT135630.20229796044935414No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT133720.19944911355369485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129050.19248360831666408No Hit
CCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGT124210.18526454079049087No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG123990.18493640135748302No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA121730.18156551445476574No Hit
GAGTACCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGC116800.17421220806963478No Hit
CCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGGGCTGATGC112010.16706771768732698No Hit
ATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGTGAGA111670.1665605931090421No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT110900.1654121050935145No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT109260.16296597477472854No Hit
CCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGCAGGCT106940.1595055952993728No Hit
GGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTCTATAATGCA105420.15723845012586385No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA104100.1552696135278166No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC103180.15389739408069278No Hit
GTACCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGCAG102600.15303229921185382No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT102450.15280856778025756No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG101560.15148109461945297No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT99900.14900513344312083No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT97290.1451122065333456No Hit
GGTCTATAATGCAAAAACCTTAGCAGAAGGTGTGCCATCAAGGTTCAGTG97100.14482881338665698No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA96610.1440979573767758No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA95740.1428003150735174No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG92590.13810195500999556No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC91560.13656566584636778No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC91540.13653583498882163No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT90910.13559616297611726No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA89410.1333588486601545No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC88410.13186730578284597No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG85700.1278252245853399No Hit
GTCTCCAGCCTCCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACAT85250.12715403029055106No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG84400.12588621884483883No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG83410.12440959139630339No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA82250.12267940165862552No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT81490.12154582907187103No Hit
GTTATTTAGCATGGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTG81270.12121768963886316No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT77690.11587796613809868No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG77110.11501287126925973No Hit
GGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTC76650.11432676154569782No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA76290.11378980610986673No Hit
GGCTTACAGGTGCCAGATGTGACATCCAGATGACTCAGTCTCCAGCCTCC75210.11217893980237355No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC75080.11198503922832345No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG74750.11149283007881162No Hit
TCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAAT73490.10961348605340289No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC71700.10694362430302067No Hit
GGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAA71010.10591445971767778No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT70890.10573547457240076No Hit
CTTCTACCCCAAAGACATCAATGTCAAGTGGAAGATTGATGGCAGTGAAC70830.10564598199976226No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70540.10521343456534277No Hit
GTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTG69580.10378155340312659No Hit
ATGCTGCACCAACTGTATCCATCTTCCCACCATCCAGTGAGCAGTTAACA69560.10375172254558043No Hit
CAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCATTGTCA69460.10360256825784958No Hit
GTGCAGCATCAGCCCGTTTTATTTCCAGCTTGGTCCCCCCTCCGAACGTG69420.10354290654275725No Hit
ATCACATGTCGAGCAAGTGAGAATATTTACAGTTATTTAGCATGGTATCA68890.10275238881778373No Hit
GGTCCTGGGGTTGCTGCTGCTGTGGCTTACAGGTGCCAGATGTGACATCC68690.10245408024232203No Hit
GATGGATACAGTTGGTGCAGCATCAGCCCGTTTTATTTCCAGCTTGGTCC68510.10218560252440649No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC68240.1017828859475332No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG67150.10015710421126689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG5600.073.23092
CGCATAG10100.071.863651
GTACAAC7700.055.6304551
AGATTAC16050.055.1752852
TCACGCA6550.054.4386864
ATAGTAC13600.053.3112224
TAGTACT13650.053.115945
TATCACG6750.051.0690272
ACAACGC8250.049.7041743
TAACGCA10750.048.095954
AGTGTAC13500.047.102913
ATCACGC7650.046.61093
GATTACT19850.045.207883
AAACGCA10950.044.5038345
GTATCAC10650.041.8969571
GACCGTT4650.038.341237
GAGTTCT18500.035.0530361
CATGGGG152000.034.875384
CATGGGT49800.034.1298524
ATTACTT26650.034.1186564
Produced by FastQC (version 0.11.5)