FastQCFastQC Report
Fri 10 Feb 2017
SRR3557268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479968
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419791.206304195900652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194260.5582235238944726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166710.4790561292517632No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA146740.4216705440969572No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121800.3500032184203993No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGG104420.3000602304389006No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT97120.27908302605081425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90580.26028974979080266No Hit
GTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGGGACAAAGTTG89060.25592189353465317No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT84440.24264590938767253No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG82660.2375309198245501No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC73030.20985825157012936No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG71800.2063237363102189No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG67440.1937948854702112No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC64300.18477181399369189No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG63090.18129477052662554No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA63020.18109361925167128No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62530.1796855603269915No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT60560.17402458873185042No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT59720.1716107734323994No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT57500.16523140442670736No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT56730.1630187404022106No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56640.16276011733441226No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC56540.1624727583701919No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG53290.1531335920330302No Hit
GATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTG52580.15109334338706562No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA51750.14870826398403664No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA49160.1412656668107293No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC48550.13951277712898508No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46060.13235753891989813No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA45650.13117936716659462No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT45530.13083453640953022No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC44810.1287655518671436No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT44760.12862187238503342No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT43860.12603564170705017No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT42870.1231907879612686No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT41820.12017351883695483No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA41140.11821947788025639No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC40770.11715624971264102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40470.11629417281997995No Hit
CCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTTGCAAGGCGAGT39800.11436886775970355No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA39580.11373667803841875No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA39460.11339184728135432No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38230.10985733202144388No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG38100.1094837653679574No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC38000.10919640640373704No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT37600.10804697054685561No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37430.10755846030768099No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC37020.10638028855437752No Hit
TCCTCCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTTGCAAGGC36480.10482855014758757No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA35920.10321933994795354No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG35660.10247220664098061No Hit
GCATGGACATGAGGGCCCCTGCTCAGTTTTTTGGGATCTTGTTGCTCTGG35560.10218484767676025No Hit
ATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCAGT35290.10140897847336527No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35140.10097794002703474No Hit
ATTTAAGCTGGTACCAGCAGAAACCATGGAAATCTCCTAAGACCCTGATC35010.10060437337354826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7500.080.1562961
TACAACG5250.069.0833742
GTACAAC5450.067.7131041
TAGTACT9400.066.409015
ATAGTAC9550.064.74344
AGTGTAC8950.064.437583
GCATAGT10150.061.5072442
AGATTAC12000.061.43892
TCACGCA4550.060.1051944
CATAGTA11650.053.0759663
ACAACGC6950.053.039253
CGCAAAG4500.052.9084431
TATCACG5200.052.5965772
GTATCAC6500.051.2804951
GATTACT14600.050.4962733
ATAACGC8750.050.2821923
TAACGCA8150.049.604024
ATCACGC5650.048.406083
ATCTACG3950.045.1572152
TACGCAG4700.044.2726755
Produced by FastQC (version 0.11.5)