FastQCFastQC Report
Fri 10 Feb 2017
SRR3557258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24692146
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2228410.9024772492435449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT999080.4046144875378592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT902490.36549678590107154No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG782820.31703198255834064No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT544880.22066935777878519No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT533140.2159148095106841No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT500690.2027729789059242No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG448790.18175414967982126No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC425570.17235034978328737No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC412470.1670450190923057No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT404490.163813222228639No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG396240.16047207885454753No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT366110.1482698182652897No Hit
CTCTAGGGGAGAAGGTCACCATGAGCTGCAGGGCCAGCTCAAGTGTAAAT355540.1439891048756961No Hit
GATATACACCGACACTGGAGAGCCAACATATGCTGAAGAGTTCAAGGGAC343330.13904421268204067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335010.13567472021265387No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA326870.13237812541688357No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT306980.12432293248225569No Hit
CTCTGAAGCCACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAG303250.12281233069009068No Hit
GTCTGGACCTGAGCTGAAGAAGCCTGGAGAGACAGTCAAGATCTCCTGCA293270.11877055967512908No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA292170.11832507389191689No Hit
GTACAATGTCTCCCTGATCATGTCTGACACAGGCGGCACCTGCTATTGAC289650.1173045064612853No Hit
GTTTACTAGTTCCCCATCTACGTTCGGTGCTGGGACCAAGCTGGAGCTGA288650.11689951938563783No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC286560.11605309639753467No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC284540.11523502250472681No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT284070.1150446785791725No Hit
GTGCTGGGACCAAGCTGGAGCTGAAACGGGCTGATGCTGCACCAACTGTA272920.11052907268570339No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG271000.10975149750046026No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG267350.10827329467434706No Hit
CCCCCAAACTATGGATTTATTACACATCCAACCTGGCTCCTGGAGTCCCA262430.10628075826216157No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC261960.10609041433660728No Hit
CATCCAACCTGGCTCCTGGAGTCCCAGCTCGCTTCAGTGGCAGTGGGTCT259560.10511844535505338No Hit
CTCTTATTCTCTCACAATCAGCAGCATGGAGGGTGAAGATGCTGCCACTT257650.10434492004056674No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA257250.10418292521030775No Hit
GTATGTGACCAGTGCCCCGATGCCAGAGCCTGGGGCCCCAGGCTTCTACT256470.10386703529130276No Hit
GCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAGC254540.10308541023530315No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT252520.10226733634249531No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA250820.10157885831389463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1141050.077.995691
GGTATCA614500.053.639751
TATCAAC1666050.053.478212
ATCAACG1706450.052.134713
TCAACGC1758900.050.6781164
CAACGCA1793000.049.711895
AACGCAG1798050.049.6615756
ACGCAGA2091450.042.717537
GCAGAGT2197050.040.6183179
CGCAGAG2275900.039.2319768
AGAGTAC2161450.033.3806810-11
GACCGTT24500.033.256797
GCATACA314250.032.0599022
AGTACTT1231050.030.47431212-13
CATACAC348700.029.8642863
TACATGG1549150.029.2501812
ACATGGG1548000.029.1062973
TGCAAAG110050.028.9666862
CATGGGA501000.028.8935414
AGACTCA106550.028.5228757
Produced by FastQC (version 0.11.5)