FastQCFastQC Report
Fri 10 Feb 2017
SRR3557251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141995
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227921.064054771369681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115980.541457846540258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95770.44710655253630377No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75760.35368896752793544No Hit
CTATTACTGTCTACAGTTTTATGAGTTTCCGCTCACGTTCGGTGCTGGGA57140.266760660038889No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT54710.2554160957425204No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54650.2551359830438446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46890.2189080740151121No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG46610.2176008814212918No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT45820.2139127308887276No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG41560.1940247292827481No Hit
AGTCAGGACTCAGCATGGACATGAGGGCTCCTGCTCAGTTTCTTGGGATC40320.18823573351011558No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC39800.18580809012159225No Hit
ACTCAAGACATTGTTAAGAATTTAAACTGGTATCAGCAGAAACCAGGGAA38370.17913207080315313No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT38180.17824504725734655No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT37860.17675111286440912No Hit
CAGTAATAGTCTGCAAAATCTTCAGACTCCAGGTTGCTGATTGTCAGAGA37790.17642431471595407No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33350.15569597501394727No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32650.15242799352939665No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32620.15228793718005876No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32040.1495801810928597No Hit
GTGCTGGGACCAAGCTGGAGCTGAAACGGGCTGATGCTGCACCAACTGTA31420.14668568320654343No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT31210.14570528876117825No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30070.14038314748633868No Hit
GTACATGGGATTGTAGTCAGGACTCAGCATGGACATGAGGGCTCCTGCTC29760.13893589854318053No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA29680.1385624149449462No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC29180.13622814245598144No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG28260.13193308107628637No Hit
GACTATTACTGTCTACAGTTTTATGAGTTTCCGCTCACGTTCGGTGCTGG28040.13090600118114187No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG26970.12591065805475737No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA26710.12469683636049571No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT26300.12278273291954463No Hit
CTCTATGTCTGCATCTCTGGGAGACAGAATAACCATCACTTGCCAGGCAA25970.1212421130768279No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT25740.12016834773190413No Hit
GTAATAGTCTGCAAAATCTTCAGACTCCAGGTTGCTGATTGTCAGAGAAT25060.11699373714691211No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24830.11591997180198835No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA24750.11554648820375397No Hit
CTATTATGCAACTGAACTGGCAGAAGGGGTCCCATCAAGGTTCAGTGGCA24680.11521969005529892No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC24430.11405255381081655No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA24200.1129787884658928No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT24040.11223182126942406No Hit
GAATAACCATCACTTGCCAGGCAACTCAAGACATTGTTAAGAATTTAAAC23770.11097131412538311No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA23580.11008429057957651No Hit
GTCTACAGTTTTATGAGTTTCCGCTCACGTTCGGTGCTGGGACCAAGCTG23190.10826355803818402No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT23180.10821687258840475No Hit
ATATTATGCCGATTCAGTGAAAGACAGATTCACCATCTCCAGAGATGATT22970.10723647814303956No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22880.1068163090950259No Hit
GTAATACATGGCTGTGTCCTCAGTTTTCAAGTTGTTCATTTGCAGATAGA21880.10214776411709645No Hit
GCATGGACATGAGGGCTCCTGCTCAGTTTCTTGGGATCTTGTTGCTCTGG21510.10042040247526254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2950.068.54742
CGCATAG5600.065.905721
ACAACGC3100.065.23063
AGATTAC8050.060.5831872
TAACGCA5050.057.708374
TATCACG3650.055.4013252
TCACGCA3550.055.2865754
ATAGTAC6900.054.303224
AAACGCA4500.052.8666275
GTACAAC3950.052.746111
GCATAGT7150.051.572682
TAGTACT7400.050.6340835
ATCACGC3900.050.3249633
ATAACGC6800.045.4808463
CATAGTA8600.043.568863
ACGCATA2200.043.292911
GTATCAC4900.042.5198251
GTATAAC7200.042.1654971
CATGGGT19550.038.6359024
ATCTACG2800.038.23392
Produced by FastQC (version 0.11.5)