FastQCFastQC Report
Fri 10 Feb 2017
SRR3557250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435604
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234200.9615684651527916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116360.47774597184107104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102070.41907469358729904No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93760.3849558466811518No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT70150.28801890619328924No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG65760.2699946296688624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65460.26876290234373074No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT56010.2299634916020831No Hit
GTCTGGACCTGAGCTGAAGAAGCCTGGAGAGACAGTCAAGATCTCCTGCA53750.22068447908609118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47740.19600887500595335No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG44920.18443063814971564No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC44200.18147449256939963No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40870.16780231926043807No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40610.16673482224532396No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39740.16316281300244212No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33520.13762499979471213No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT32880.1349973148344312No Hit
GTCTTAGACATCATGGGTTGGCTGTGGAACTTGCTATTCCTGATGGCAGC32770.13454568148188292No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32310.13265703291668104No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG31750.13035780857643525No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG31000.12727849026360608No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC30390.12477397803583835No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT30220.12407599921826372No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG29860.12259792642810571No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC29440.12087350817292138No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA28940.11882062929770192No Hit
CTCTGAAGCCACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAG28420.1166856352674737No Hit
ACATATTTCTGTGCAAGATCGGATTACTACGGTAGTAGCTACGGGTACTA27230.1117997835444514No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27180.11159449565692944No Hit
CAGTATCAACAGTGTGGAACCTGAAGATGTTGGAGTGTATTACTGTCAAA27120.11134815019190313No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG26260.10781719852652565No Hit
GTATCAACAGTGTGGAACCTGAAGATGTTGGAGTGTATTACTGTCAAAAT25870.10621595300385447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG3200.081.7730942
CGCATAG5000.079.766921
GTACAAC4200.062.3621981
AGATTAC8850.057.7913632
ACAACGC4650.057.551733
TAACGCA6000.057.487784
TAGTACT7550.055.928995
GCATAGT7350.055.0211032
ATAGTAC7350.055.0199744
AGTGTAC6650.050.974393
TATCACG3250.049.406962
TCACGCA3500.047.5760964
GTATCAC4400.047.3514671
CATAGTA9100.044.439213
AAACGCA6750.043.173565
GACCGTT2800.042.4812747
ATAACGC9050.040.741963
ATCACGC3950.040.650463
TTAGGAC3350.039.0550083
ATTACTT14000.036.5316474
Produced by FastQC (version 0.11.5)