FastQCFastQC Report
Fri 10 Feb 2017
SRR3557249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919369
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175990.9169159239312503No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132870.6922587579563909No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92500.4819292173625811No Hit
GATATGGGTGTACCCACTCAGCTCCTGTTGCTGTGGCTTACAGTCGTAGT79520.4143028255640265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78450.4087280767793999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77650.4045600403049127No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT66200.34490501826381487No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC63750.3321404065606978No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59540.310206114613709No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59300.3089557036713628No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT53530.278893740599124No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50830.26482661749772973No Hit
GAACACACACAGATATGGGTGTACCCACTCAGCTCCTGTTGCTGTGGCTT48470.25253090989799254No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG48390.2521141062505438No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT46150.24044360412197968No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG43800.22819999697817356No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC40360.21027744013787866No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT39510.20584890138373602No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG36430.18980196095696034No Hit
GTCTGCATCTGTGGGAGAAACTGTCACCATCACATGTGGAGCAAGTGAGA35600.18547762311467988No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35250.18365410715709174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.17974657296226No Hit
CAGATATGGGTGTACCCACTCAGCTCCTGTTGCTGTGGCTTACAGTCGTA34260.17849616201991383No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31550.1643769384625885No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA31510.16416853663886413No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA30760.16026100244403238No Hit
GCTTTAAATTGGTATCAGCGGAAACAGGGAAAATCTCCTCAGCTCCTGAT30240.1575517787356157No Hit
GCCTCACACAGAACACACACAGATATGGGTGTACCCACTCAGCTCCTGTT29920.15588456414582086No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT29800.15525935867464777No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC28410.14801739530022628No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT27480.1431720528986349No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA26600.13858721277669903No Hit
GTAATACGTTGCAACATCGTCAGGATGCAGGCTACTGATCTTGAGAGAAT26480.13796200730552594No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG25370.13217885669717497No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25170.13113684757855318No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25110.13082424484296662No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG24580.12806292067861885No Hit
ACGTATTACTGTCAAAATGTGTTAAGTACTCCGTACACGTTCGGAGGGGG24460.1274377152074458No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG24430.1272814138396525No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT24380.12702091155999706No Hit
GTAATATATGGCTGTGTCATCAGCTTGCAGACTGTTCATTTTAAAGAAAA23990.12498899377868455No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT23980.12493689332275346No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC23830.12415538648378713No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT23550.12269657371771661No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA23310.12144616277537044No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT23140.12056045502454192No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT23120.12045625411267974No Hit
GTATTACTGTCAAAATGTGTTAAGTACTCCGTACACGTTCGGAGGGGGGA23110.12040415365674866No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA22620.11785123131612524No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA22540.11743442766867652No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA21740.11326639119418934No Hit
GATCTATGGTGCAACCAACTTGGCAGATGGCATGTCATCGAGGTTCAGTG21670.11290168800267171No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC21560.11232858298742972No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC21530.11217228161963645No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC21050.10967145973494413No Hit
GTATTCTCTCAAGATCAGTAGCCTGCATCCTGACGATGTTGCAACGTATT20450.10654543237907875No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC20440.10649333192314767No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA20320.10586812645197459No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC20300.10576392554011241No Hit
GTGTTAAGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAA19590.10206479316900502No Hit
GTACAATGTCTCCCTGATCATGTCTGACACAGGCGGCACCTGCTATTGAC19490.10154378860969411No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA19230.1001891767554858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3900.076.3311
TACAACG2000.068.393992
GTACAAC2450.055.8929821
TCACGCA2450.055.8303764
GCATAGT5450.054.562422
ATAGTAC5650.053.6822284
TAGTACT5950.052.97465
TAACGCA5850.051.846944
AGATTAC5450.051.2886732
TATCACG2600.050.323342
GTATAAC6250.049.5357671
ATAACGC6200.048.9200973
ACAACGC2950.048.3835873
GTATCAC3250.047.6305431
AAACGCA4150.045.8575335
CATAGTA6650.044.7154053
TACTGGT6150.043.5168572
GTACTGG7750.043.0211331
CATGGGT21450.042.974734
TTAGGAC2300.041.3714683
Produced by FastQC (version 0.11.5)