##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557244.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2595629 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.548402333307266 33.0 33.0 34.0 32.0 34.0 2 32.61446377737342 33.0 33.0 34.0 31.0 34.0 3 32.66689076135303 33.0 33.0 34.0 32.0 34.0 4 32.609490416388475 33.0 33.0 34.0 32.0 34.0 5 32.655295498701854 34.0 33.0 34.0 32.0 34.0 6 36.047178930424955 38.0 37.0 38.0 31.0 38.0 7 36.32341717556708 38.0 37.0 38.0 33.0 38.0 8 36.53023756476754 38.0 38.0 38.0 34.0 38.0 9 36.59912260188186 38.0 38.0 38.0 34.0 38.0 10-11 36.51090294491239 38.0 38.0 38.0 34.0 38.0 12-13 36.5181651538028 38.0 38.0 38.0 34.0 38.0 14-15 36.51228372775924 38.0 38.0 38.0 34.0 38.0 16-17 36.490499605297984 38.0 38.0 38.0 34.0 38.0 18-19 36.58014319457827 38.0 38.0 38.0 34.0 38.0 20-21 36.475444487636715 38.0 38.0 38.0 34.0 38.0 22-23 36.38378809144142 38.0 38.0 38.0 33.5 38.0 24-25 36.37213927722337 38.0 38.0 38.0 33.0 38.0 26-27 36.22765445292836 38.0 38.0 38.0 33.0 38.0 28-29 36.16442411454025 38.0 38.0 38.0 33.0 38.0 30-31 36.04357864702544 38.0 38.0 38.0 32.5 38.0 32-33 35.827382688358 38.0 37.5 38.0 30.0 38.0 34-35 35.40156721164696 38.0 37.0 38.0 28.0 38.0 36-37 35.12239634400756 38.0 37.0 38.0 26.5 38.0 38-39 34.775136585390285 38.0 36.0 38.0 24.5 38.0 40-41 34.34689992290886 38.0 35.5 38.0 16.0 38.0 42-43 33.77598416414672 38.0 34.5 38.0 15.0 38.0 44-45 33.15977861242882 38.0 33.0 38.0 15.0 38.0 46-47 32.371136437449266 38.0 28.5 38.0 15.0 38.0 48-49 31.557714526999042 38.0 25.5 38.0 15.0 38.0 50-51 30.900636030804094 38.0 24.0 38.0 15.0 38.0 52-53 30.464336968033564 38.0 20.0 38.0 14.5 38.0 54-55 30.040515612978588 38.0 15.0 38.0 14.0 38.0 56-57 29.73747769808397 37.0 15.0 38.0 14.0 38.0 58-59 29.479809325600847 37.0 15.0 38.0 14.0 38.0 60-61 29.233382736901152 37.0 15.0 38.0 14.0 38.0 62-63 29.026721461349062 36.0 15.0 38.0 14.0 38.0 64-65 28.739044948257245 36.0 15.0 38.0 14.0 38.0 66-67 28.488992070900736 35.5 15.0 38.0 14.0 38.0 68-69 28.20799640472502 34.5 15.0 38.0 14.0 38.0 70-71 27.874463184068293 34.0 14.0 38.0 14.0 38.0 72-73 27.493494640412784 34.0 14.0 38.0 13.5 38.0 74-75 27.24684845176256 33.0 14.0 38.0 13.0 38.0 76-77 27.10844635346577 33.0 14.0 38.0 13.0 38.0 78-79 27.012502557183634 33.0 14.0 38.0 13.0 38.0 80-81 26.90818564594555 33.0 14.0 38.0 13.0 38.0 82-83 26.847417523844896 32.0 14.0 38.0 13.0 38.0 84-85 26.76613106110311 31.0 14.0 38.0 13.0 38.0 86-87 26.68330816923374 31.0 14.0 38.0 13.0 38.0 88-89 26.612552679909186 29.5 14.0 38.0 13.0 38.0 90-91 26.517756197052815 29.0 14.0 38.0 13.0 38.0 92-93 26.418645345694628 28.5 14.0 38.0 13.0 38.0 94-95 26.362589183585172 28.0 14.0 38.0 13.0 38.0 96-97 26.271751856679053 27.0 14.0 38.0 13.0 38.0 98-99 26.154310381029028 25.5 14.0 38.0 13.0 38.0 100-101 26.05242891029496 25.0 14.0 38.0 13.0 38.0 102-103 25.930456163034087 24.0 14.0 38.0 13.0 38.0 104-105 25.814311868144486 24.0 14.0 38.0 13.0 38.0 106-107 25.62431591725936 24.0 14.0 38.0 13.0 38.0 108-109 25.373823840001787 23.0 14.0 38.0 13.0 38.0 110-111 25.126891978784336 23.0 14.0 38.0 13.0 38.0 112-113 24.922273368035263 23.0 14.0 38.0 13.0 38.0 114-115 24.782311339563552 23.0 14.0 38.0 13.0 38.0 116-117 24.536560309659045 23.0 13.0 38.0 13.0 38.0 118-119 24.288610583407724 23.0 13.0 38.0 13.0 38.0 120-121 24.13005787807117 23.0 13.0 38.0 13.0 38.0 122-123 24.045202723501703 23.0 13.0 38.0 13.0 38.0 124-125 23.057368753392723 21.5 13.0 35.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13792609552662327 1101 2 -0.03997402208961631 1101 3 -0.14317840738925014 1101 4 -0.12993285121171283 1101 5 -0.03246997169610921 1101 6 0.22194126980999584 1101 7 0.2683903467176094 1101 8 0.035877737542385546 1101 9 0.183534102195253 1101 10-11 0.18573102587115642 1101 12-13 0.24524037278659705 1101 14-15 0.0655045087530226 1101 16-17 0.18086833360464283 1101 18-19 0.20095494447214435 1101 20-21 0.2350405720096589 1101 22-23 0.32693043655184084 1101 24-25 0.18052291202401705 1101 26-27 0.18282748539562732 1101 28-29 0.0169300675829831 1101 30-31 0.13661452524718953 1101 32-33 0.18363695807634173 1101 34-35 0.11622208606088691 1101 36-37 -0.0344232070959265 1101 38-39 0.007845020973256567 1101 40-41 0.022851649635832416 1101 42-43 0.10721743189664323 1101 44-45 0.17795842562121322 1101 46-47 0.11393459824058283 1101 48-49 0.1683032678524583 1101 50-51 0.0016041204015984079 1101 52-53 0.2690863439015736 1101 54-55 0.26676356702649784 1101 56-57 0.021091006501233522 1101 58-59 -0.033390446706089705 1101 60-61 0.035903390971643745 1101 62-63 0.0927407795253039 1101 64-65 0.09775541122191811 1101 66-67 0.17736792101014132 1101 68-69 0.26541735732301674 1101 70-71 0.33936415291063327 1101 72-73 0.1522756099501663 1101 74-75 0.24468561628313878 1101 76-77 0.0730864818363699 1101 78-79 0.26245498829125324 1101 80-81 0.17915761633442884 1101 82-83 0.1494737034611262 1101 84-85 0.29391438406113934 1101 86-87 0.26036602212013094 1101 88-89 0.158928713090301 1101 90-91 0.29812181061581455 1101 92-93 0.2907726647082569 1101 94-95 0.29619235589660775 1101 96-97 0.17084540292713157 1101 98-99 0.054251802781511316 1101 100-101 0.3070878497251286 1101 102-103 0.12144597494147291 1101 104-105 0.048633066074440734 1101 106-107 -0.1326465601467497 1101 108-109 0.24041711698970403 1101 110-111 0.04942557276015691 1101 112-113 0.20980752996556262 1101 114-115 0.23542818891148087 1101 116-117 0.13374590264762887 1101 118-119 0.2162212783055395 1101 120-121 0.05485778189799717 1101 122-123 0.1991460788864572 1101 124-125 -0.0925958962291169 1102 1 -0.28711400218845995 1102 2 -0.17031175926397424 1102 3 -0.17542755771487606 1102 4 -0.2652511193225635 1102 5 -0.2639853970701651 1102 6 -0.018698293772793306 1102 7 0.09596796572048305 1102 8 -0.024958201856705386 1102 9 0.15736636849565144 1102 10-11 0.2451168891388278 1102 12-13 0.16058327735313327 1102 14-15 -0.11849954164731713 1102 16-17 -0.07267218754886073 1102 18-19 0.031157643446576344 1102 20-21 0.10696589909142062 1102 22-23 0.16102143037631578 1102 24-25 0.14112456648430083 1102 26-27 0.10139654278275856 1102 28-29 -0.32271471707917954 1102 30-31 -0.16055593606000684 1102 32-33 -0.18663651851232288 1102 34-35 -0.10347082320328127 1102 36-37 -0.28503263128588685 1102 38-39 -0.3005666434639025 1102 40-41 -0.22400757509129932 1102 42-43 -0.41918555609382935 1102 44-45 -0.2924398736878544 1102 46-47 -0.4229282951113049 1102 48-49 -0.5503085155913077 1102 50-51 -0.5809746841934285 1102 52-53 -0.29818102278641945 1102 54-55 -0.1706078633345065 1102 56-57 -0.39753628657565443 1102 58-59 -0.3596165652265313 1102 60-61 -0.2827195773037019 1102 62-63 -0.4679120268980661 1102 64-65 -0.41143747641825 1102 66-67 -0.27866801063413504 1102 68-69 -0.3384209786126533 1102 70-71 -0.28039482142539995 1102 72-73 -0.343717237722867 1102 74-75 -0.4080047085392806 1102 76-77 -0.23971470703666142 1102 78-79 -0.2515440960438653 1102 80-81 -0.24248943213764917 1102 82-83 -0.3425984879158186 1102 84-85 -0.23621173609974022 1102 86-87 -0.42049136232937556 1102 88-89 -0.4855687226006431 1102 90-91 -0.27477175005294896 1102 92-93 -0.2573701312908554 1102 94-95 -0.05425004147510748 1102 96-97 -0.4075051625033872 1102 98-99 -0.4433158691778196 1102 100-101 -0.24748659725951683 1102 102-103 -0.25173548329212636 1102 104-105 -0.4335515850557492 1102 106-107 -0.4973121858784211 1102 108-109 -0.40518060786641996 1102 110-111 -0.43481097300593774 1102 112-113 -0.5170752127340954 1102 114-115 -0.17480667763856417 1102 116-117 -0.3375555345286614 1102 118-119 -0.17103986961609507 1102 120-121 -0.4216589185468642 1102 122-123 -0.3158133350880199 1102 124-125 -0.3489551440715637 1103 1 -0.08016287045654735 1103 2 -0.14159092471012258 1103 3 -0.051626816720080626 1103 4 -0.12464375088446644 1103 5 -0.0745013702624675 1103 6 0.09051924575355486 1103 7 0.20605147115282563 1103 8 -0.035114570305104564 1103 9 0.0644602822994429 1103 10-11 0.2200501716397838 1103 12-13 0.20429343269120182 1103 14-15 0.0775530492584906 1103 16-17 0.02273707444445705 1103 18-19 0.060905257734788165 1103 20-21 0.050993413343867644 1103 22-23 0.12889494901232723 1103 24-25 0.11478966451082329 1103 26-27 0.06128390686379248 1103 28-29 -0.01685557380104541 1103 30-31 0.03793712428971219 1103 32-33 0.051428666655418454 1103 34-35 0.09748110634185991 1103 36-37 0.0455745457947998 1103 38-39 -0.258731864400751 1103 40-41 -0.08291401161877587 1103 42-43 0.007695082510025486 1103 44-45 -0.03049534378678942 1103 46-47 -0.12535110079373624 1103 48-49 -0.04746251562368897 1103 50-51 -0.08108346352447526 1103 52-53 -0.13562746110971702 1103 54-55 0.07445393775093478 1103 56-57 -0.07777267186351011 1103 58-59 -0.00840836394208111 1103 60-61 0.023727033241268458 1103 62-63 -0.2557480247607522 1103 64-65 -0.011289836811975817 1103 66-67 -0.013834572544883628 1103 68-69 0.10567512991350014 1103 70-71 0.0054048391359842185 1103 72-73 -0.19584873853908036 1103 74-75 -0.15977873898277295 1103 76-77 -0.2320117614572368 1103 78-79 -0.15434233025651167 1103 80-81 -0.1819705149851103 1103 82-83 -0.13984279392870747 1103 84-85 -0.077380370558501 1103 86-87 0.02673991665943376 1103 88-89 -0.1771155823518491 1103 90-91 -0.059867877714840034 1103 92-93 -0.040891313820807795 1103 94-95 0.041728950424584355 1103 96-97 -0.11415299273108914 1103 98-99 -0.14163427908600923 1103 100-101 -0.12345953932090836 1103 102-103 -0.2078941745251619 1103 104-105 -0.22326185520826058 1103 106-107 -0.2246995536394074 1103 108-109 -0.1964245341084414 1103 110-111 -0.2924997875761868 1103 112-113 -0.12427341632955446 1103 114-115 0.008862291695670876 1103 116-117 -0.1311433683185541 1103 118-119 -0.28862686732127685 1103 120-121 -0.11519964897283685 1103 122-123 -0.08824469297496407 1103 124-125 0.24861822309768655 1104 1 -0.01563621614315025 1104 2 -0.029736065425758795 1104 3 -0.08350051252531188 1104 4 0.023218599040937704 1104 5 -0.07396190294955574 1104 6 0.014030012248383628 1104 7 0.3108879040850354 1104 8 0.20220592303124363 1104 9 0.04074266697058704 1104 10-11 0.2263384369385193 1104 12-13 0.23886395885840273 1104 14-15 0.18697148324707058 1104 16-17 0.1971856173620523 1104 18-19 0.18391190431919568 1104 20-21 0.23294272135049 1104 22-23 0.25632978338573054 1104 24-25 0.20785197023750612 1104 26-27 0.11356016966566074 1104 28-29 -0.017576623208157116 1104 30-31 0.026007448265140454 1104 32-33 0.1193765995265963 1104 34-35 0.1724748005040979 1104 36-37 0.005339612842050201 1104 38-39 0.06400893905554028 1104 40-41 0.06868874142899273 1104 42-43 -0.009639824582997392 1104 44-45 0.12007338203480344 1104 46-47 0.26878285024172754 1104 48-49 0.3179192016121313 1104 50-51 0.205960006838378 1104 52-53 0.25829099225919094 1104 54-55 0.30821871958919544 1104 56-57 0.3141485230985346 1104 58-59 0.2617516597077021 1104 60-61 0.4154656337039704 1104 62-63 0.24172935087684522 1104 64-65 0.24663621075255548 1104 66-67 0.28178599420530404 1104 68-69 0.2524277391708125 1104 70-71 0.21104095642919418 1104 72-73 0.23527661630892283 1104 74-75 0.33497649616423075 1104 76-77 0.28257482724073313 1104 78-79 0.3193182143480371 1104 80-81 0.2988104196326091 1104 82-83 0.28037360622471397 1104 84-85 0.3002677197272092 1104 86-87 0.2769557758992498 1104 88-89 0.30055509903152355 1104 90-91 0.37481637047599037 1104 92-93 0.37179597389953045 1104 94-95 0.5115364256770647 1104 96-97 0.3714848832544888 1104 98-99 0.25989948578260424 1104 100-101 0.34451295160649664 1104 102-103 0.2711504852691071 1104 104-105 0.2914867906275411 1104 106-107 0.3185117781245985 1104 108-109 0.2527042768014667 1104 110-111 0.27737901885021543 1104 112-113 0.39647814118344726 1104 114-115 0.4035274661735784 1104 116-117 0.4307777890398654 1104 118-119 0.5230120459456842 1104 120-121 0.4263001395791157 1104 122-123 0.4400377848374539 1104 124-125 0.29013883826976183 1105 1 0.16300838241668458 1105 2 0.061300337865368704 1105 3 -0.02543114220122078 1105 4 -0.010840664912109332 1105 5 0.03981211017561748 1105 6 0.09885544791887924 1105 7 0.2450109078668774 1105 8 0.04032860825768836 1105 9 0.16961916695534995 1105 10-11 0.19936092096907032 1105 12-13 0.30488650441498777 1105 14-15 0.16875402491658775 1105 16-17 0.2305093001153864 1105 18-19 0.1978285773588908 1105 20-21 0.2529753416525793 1105 22-23 0.34143733320601655 1105 24-25 0.3504411585595193 1105 26-27 0.2903276489552411 1105 28-29 0.0682754204626832 1105 30-31 0.19209965625076109 1105 32-33 0.18120603036471294 1105 34-35 0.2995370729080591 1105 36-37 0.13991539366214312 1105 38-39 0.06778797489072019 1105 40-41 0.15310189512162964 1105 42-43 0.22518372793062014 1105 44-45 -0.041473086514173474 1105 46-47 -0.1402316348284387 1105 48-49 -0.09911846612539321 1105 50-51 -0.16340353213951886 1105 52-53 -0.020653703879791152 1105 54-55 0.11894233483638317 1105 56-57 0.040138201098830706 1105 58-59 -0.15721854386299228 1105 60-61 0.040763416868948354 1105 62-63 0.09613659921952333 1105 64-65 0.12732003820133642 1105 66-67 0.14964789526797873 1105 68-69 0.16042891334068798 1105 70-71 0.3491075494562814 1105 72-73 0.1948774388028447 1105 74-75 0.12081404958981778 1105 76-77 0.06806414039735742 1105 78-79 0.061476806632207115 1105 80-81 0.14551124379406133 1105 82-83 0.11713236836676089 1105 84-85 0.13406877556008112 1105 86-87 0.09400327630319438 1105 88-89 0.13489767027953903 1105 90-91 0.23513730640783237 1105 92-93 0.12234065186820686 1105 94-95 0.20741234087692817 1105 96-97 0.2478070462570976 1105 98-99 0.009343307619285923 1105 100-101 0.07219172069466495 1105 102-103 0.08450060121981906 1105 104-105 -0.012341667294105463 1105 106-107 -0.0815660494426318 1105 108-109 0.16087737888122078 1105 110-111 0.13577165964566262 1105 112-113 0.20855130427173307 1105 114-115 0.43913595009716744 1105 116-117 0.4603710961565888 1105 118-119 0.3983665394454867 1105 120-121 0.4038866914815067 1105 122-123 0.4317174658910581 1105 124-125 0.5404004676978431 1106 1 0.11138376324838362 1106 2 0.07139367998975388 1106 3 -0.07746734957636647 1106 4 -0.028095698140397474 1106 5 -0.033721839094873474 1106 6 -0.012983090137822728 1106 7 0.302521632526755 1106 8 0.25645591232115095 1106 9 0.08109490492849858 1106 10-11 0.2798494300006382 1106 12-13 0.2677242699696123 1106 14-15 0.13970193620497895 1106 16-17 0.19089625778035924 1106 18-19 0.16127218273644672 1106 20-21 0.16019640618522857 1106 22-23 0.4441126143218881 1106 24-25 0.2704944426223861 1106 26-27 0.2735286143576232 1106 28-29 0.11907003625832147 1106 30-31 0.16107070544033775 1106 32-33 0.3625539901878483 1106 34-35 0.2845068541131752 1106 36-37 0.2623538764275182 1106 38-39 -0.04638221598823833 1106 40-41 0.09728130028376114 1106 42-43 -0.03952294931384159 1106 44-45 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-0.23159176951944005 2313 22-23 -0.3943028528734942 2313 24-25 -0.32832729419096296 2313 26-27 -0.032394429040131456 2313 28-29 -0.10531144754825306 2313 30-31 -0.02994105065788233 2313 32-33 -0.19168868313894905 2313 34-35 -0.15892962547059852 2313 36-37 0.1157015755653461 2313 38-39 0.3424547974298804 2313 40-41 0.05183650607193613 2313 42-43 0.08347093778606762 2313 44-45 -0.003940736252545207 2313 46-47 0.19831133357513409 2313 48-49 0.0044376439420865665 2313 50-51 0.36395158706262976 2313 52-53 0.3313710596911932 2313 54-55 0.09443978309611722 2313 56-57 0.11853719572123111 2313 58-59 0.1397467798814951 2313 60-61 0.13448242713234393 2313 62-63 0.0735906840195355 2313 64-65 0.07370073930507459 2313 66-67 0.16911499273397368 2313 68-69 0.20349658484746413 2313 70-71 -0.06624259929445486 2313 72-73 -0.09894910332707241 2313 74-75 -0.10186509628265128 2313 76-77 0.0458568447582941 2313 78-79 0.06314722901979763 2313 80-81 -0.08920552513033897 2313 82-83 0.03907098550897459 2313 84-85 0.10274948956361385 2313 86-87 0.08347130094179889 2313 88-89 0.051147781137135695 2313 90-91 0.03229511924796569 2313 92-93 0.08763315466988075 2313 94-95 0.21516279841645414 2313 96-97 0.20887637861219588 2313 98-99 0.3321349235602078 2313 100-101 0.15911818381335152 2313 102-103 0.135990426508247 2313 104-105 0.12389727718035104 2313 106-107 0.16909708053084316 2313 108-109 0.15104834144763402 2313 110-111 0.36708358872904867 2313 112-113 0.0261331171763608 2313 114-115 0.007708930674915848 2313 116-117 0.020394436719183062 2313 118-119 0.15285727981297015 2313 120-121 0.17010666922936224 2313 122-123 0.2616347056578938 2313 124-125 -0.20438519418246415 2314 1 0.08338152917663422 2314 2 2.997026518372081E-4 2314 3 0.02085706900453488 2314 4 0.094749397199692 2314 5 0.02448094791791533 2314 6 -0.07949310674151633 2314 7 -0.21527543247943726 2314 8 -0.3144152652268133 2314 9 -0.31218937932096225 2314 10-11 -0.2297065965542231 2314 12-13 -0.20076909269488397 2314 14-15 -0.05552486128133438 2314 16-17 -0.3465693766634459 2314 18-19 -0.3657678295658542 2314 20-21 -0.320056823583478 2314 22-23 -0.32165419795148154 2314 24-25 -0.4418312034279879 2314 26-27 -0.1712886125110913 2314 28-29 -0.032999033885239726 2314 30-31 -0.10920207383657754 2314 32-33 -0.08163507655435609 2314 34-35 -0.07235939643967981 2314 36-37 0.06758434307021588 2314 38-39 0.0885980826573487 2314 40-41 -0.14038506103400294 2314 42-43 -0.2742918090261739 2314 44-45 -0.3158441427442966 2314 46-47 -6.841924324234583E-4 2314 48-49 -0.12484461404044822 2314 50-51 0.21298702715958484 2314 52-53 -0.08234172433842346 2314 54-55 -0.11925816198480277 2314 56-57 -0.023672384450886597 2314 58-59 -0.21953537079751229 2314 60-61 -0.08498689478841115 2314 62-63 -0.17900147277012124 2314 64-65 -0.0351234653216288 2314 66-67 -0.11753078645343251 2314 68-69 -0.38415959826431845 2314 70-71 -0.32708915072842615 2314 72-73 -0.31386727309029183 2314 74-75 -0.3248907536861978 2314 76-77 -0.27671585055954395 2314 78-79 -0.2225981888370896 2314 80-81 -0.1235987067251827 2314 82-83 -1.4781937819208224E-4 2314 84-85 -0.008099151465415844 2314 86-87 -0.06884387155144722 2314 88-89 -0.03145871196889516 2314 90-91 -0.11344298220243942 2314 92-93 -0.2782960261297944 2314 94-95 -0.24073538013436035 2314 96-97 -0.11126250321725095 2314 98-99 0.07137273246730658 2314 100-101 -0.15871571935820228 2314 102-103 -0.07479246287084962 2314 104-105 0.11104810302220258 2314 106-107 -0.07258153071298423 2314 108-109 -0.005962278213310412 2314 110-111 0.09397753839126821 2314 112-113 -0.27732846646560816 2314 114-115 -0.01646591929780783 2314 116-117 0.13191993712072403 2314 118-119 0.4725757718256318 2314 120-121 0.21123759478443205 2314 122-123 0.4076269470479126 2314 124-125 0.5445330168079003 2315 1 0.037262265882709755 2315 2 -0.09075776514501399 2315 3 0.017608872462218983 2315 4 -0.061127288981786876 2315 5 -0.05604185148767726 2315 6 -0.45576259894748006 2315 7 -0.43244496782851627 2315 8 -0.3344946890091407 2315 9 -0.5667489969382231 2315 10-11 -0.49645076130195775 2315 12-13 -0.5373343851486538 2315 14-15 -0.3116169211962827 2315 16-17 -0.6170998518117088 2315 18-19 -0.6349976017619525 2315 20-21 -0.5789755480228891 2315 22-23 -0.7238884672012702 2315 24-25 -0.6195285632374308 2315 26-27 -0.3153031498464145 2315 28-29 -0.4053006129460286 2315 30-31 -0.3035655804303161 2315 32-33 -0.35226418691882344 2315 34-35 -0.29642364022662804 2315 36-37 0.002729658737806062 2315 38-39 -0.018879478009004913 2315 40-41 -0.423031003053012 2315 42-43 -0.42447086933238865 2315 44-45 -0.5861779884110234 2315 46-47 -0.32167209996855917 2315 48-49 -0.36871872616326584 2315 50-51 -0.2604051084302945 2315 52-53 -0.4121898517435021 2315 54-55 -0.4125542147672512 2315 56-57 -0.30677169238062874 2315 58-59 -0.37778022798582356 2315 60-61 -0.45917892281249095 2315 62-63 -0.48384617820975606 2315 64-65 -0.4640274863482361 2315 66-67 -0.24335314421539778 2315 68-69 -0.2956504086669227 2315 70-71 -0.48164576299830486 2315 72-73 -0.46095915795443787 2315 74-75 -0.23858721844337083 2315 76-77 -0.2519812006561608 2315 78-79 -0.4135329643953227 2315 80-81 -0.23740701028635414 2315 82-83 -0.3675211272501073 2315 84-85 -0.41901510016029064 2315 86-87 -0.2189028603959109 2315 88-89 -0.30560450607069356 2315 90-91 -0.25492013750298526 2315 92-93 -0.2578271178727931 2315 94-95 -0.24727877628230033 2315 96-97 -0.22511446265388813 2315 98-99 -0.047321753176035486 2315 100-101 -0.2866094878772323 2315 102-103 -0.21730432651524723 2315 104-105 -0.22184026797838996 2315 106-107 -0.06448995336281982 2315 108-109 -0.4623280526336444 2315 110-111 -0.052319567402228984 2315 112-113 -0.12538835805375825 2315 114-115 0.021681125940077095 2315 116-117 -0.04368393473525245 2315 118-119 0.33020078679895803 2315 120-121 0.005771893418252461 2315 122-123 0.025938856242550656 2315 124-125 0.37965054369472284 2316 1 -0.056805488198733656 2316 2 -0.15437174768828754 2316 3 -0.1508306919415432 2316 4 -0.1435261957001046 2316 5 -0.15988848367533848 2316 6 -1.0288358099824109 2316 7 -0.8752159045146186 2316 8 -1.1933419284947036 2316 9 -1.0394730298617674 2316 10-11 -0.9103859571609831 2316 12-13 -0.7299195323147529 2316 14-15 -0.5533731249079992 2316 16-17 -0.6892712072788143 2316 18-19 -0.6715879185968987 2316 20-21 -0.7686848668819266 2316 22-23 -0.8217978350805097 2316 24-25 -0.7594842559461483 2316 26-27 -0.2944713771838323 2316 28-29 -0.17558592659175787 2316 30-31 -0.22311555450197318 2316 32-33 -0.3470582983838142 2316 34-35 -0.28328956848692144 2316 36-37 -0.1513517831837845 2316 38-39 -0.07172450503922079 2316 40-41 -0.18106031772278897 2316 42-43 -0.40251766836332337 2316 44-45 -0.3268815666684475 2316 46-47 -0.2814525417759697 2316 48-49 -0.41211717977188655 2316 50-51 -0.22417909760657295 2316 52-53 -0.277112543084165 2316 54-55 -0.31784068846106805 2316 56-57 -0.49190925808334995 2316 58-59 -0.2884491793532611 2316 60-61 -0.2194060740471251 2316 62-63 -0.19764835037253192 2316 64-65 -0.2296603889485631 2316 66-67 -0.30465139439740696 2316 68-69 -0.5320396989865941 2316 70-71 -0.32935850454467896 2316 72-73 -0.42005375314856863 2316 74-75 -0.3532366984581792 2316 76-77 -0.09261888527290907 2316 78-79 -0.20105579261438677 2316 80-81 -0.29409830585278485 2316 82-83 -0.1442571810356128 2316 84-85 -0.29497592180889853 2316 86-87 -0.3452691897637514 2316 88-89 -0.1751982274104158 2316 90-91 -0.31916047328417463 2316 92-93 -0.4165262784925403 2316 94-95 -0.22210260534285453 2316 96-97 -0.2673657414341726 2316 98-99 -0.35095215821828063 2316 100-101 -0.3103693091934616 2316 102-103 -0.39291415179527434 2316 104-105 -0.16274429090879394 2316 106-107 -0.28568741391209684 2316 108-109 -0.11480600990169165 2316 110-111 -0.12873080772273937 2316 112-113 -0.21630630883425894 2316 114-115 -0.17724050208377662 2316 116-117 0.09262513407705697 2316 118-119 0.23470584628142177 2316 120-121 0.32214442667963183 2316 122-123 -0.05104920798993007 2316 124-125 0.4430242980976331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 140.0 15 1494.0 16 2711.0 17 3461.0 18 6205.0 19 15588.0 20 44635.0 21 96746.0 22 167321.0 23 253131.0 24 261671.0 25 164733.0 26 105716.0 27 74807.0 28 49531.0 29 42214.0 30 45320.0 31 50856.0 32 64779.0 33 88451.0 34 128536.0 35 159089.0 36 232338.0 37 536155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.807635199916085 16.247476800447984 12.182821185851726 16.76206681378421 2 14.880604013425089 24.965102242819036 39.33793088568061 20.816362858075262 3 21.39461929884518 37.83347158751374 25.695396694460058 15.076512419181023 4 12.459022456599152 18.33023902876721 34.76066880128093 34.45006971335272 5 11.542828347194456 41.625401781225285 27.2632568059611 19.56851306561916 6 24.639923502164603 35.34172256512776 21.710922477750096 18.307431454957545 7 24.09689520343624 37.20863035510853 20.924947286380295 17.769527155074936 8 30.676263826610047 24.309213681924497 22.01169735736502 23.002825134100444 9 35.03289568732665 12.456787930786719 21.440621907059906 31.06969447482672 10-11 23.194724669819916 28.037076947437406 24.96458469218829 23.80361369055439 12-13 28.02852410725878 26.80566059325119 29.478172728074775 15.687642571415253 14-15 26.315124387961454 22.921650205017745 33.89596510132996 16.867260305690838 16-17 16.73937222923615 27.985875485287 32.070935407178766 23.203816878298092 18-19 17.732811584398235 24.29503600090768 41.21613682078602 16.756015593908067 20-21 23.14125636770441 22.74969251054903 38.751414644106845 15.357636477639714 22-23 20.046774560500367 26.469214781223847 36.656004534780145 16.828006123495637 24-25 17.712989799389668 28.065085572707037 38.303644318968544 15.91828030893475 26-27 18.62964828800024 27.406320894712355 38.6874909506367 15.276539866650705 28-29 18.188444219065374 28.604322551359584 38.49250173594119 14.714731493633854 30-31 19.09219469053484 28.939318664421528 37.126341414456256 14.84214523058738 32-33 15.499344558195624 29.940658678078385 38.18614547244303 16.37385129128296 34-35 14.640283851739325 31.61735150750451 36.85148967220368 16.890874968552488 36-37 15.95059912551726 32.622777561932246 35.335801681128316 16.090821631422177 38-39 15.823152648301756 31.54587510455053 35.51124847065636 17.119723776491355 40-41 14.945338007195774 32.88976343966679 35.158983774051435 17.005914779086005 42-43 15.248091404509731 32.92120591869786 34.637891857879424 17.192810818912985 44-45 15.717251271301954 32.12641447092743 34.20124476331484 17.955089494455784 46-47 14.481618465255771 31.405405353328753 35.03489018479321 19.078085996622256 48-49 14.215840500279459 31.294111663136736 34.654598440691906 19.8354493958919 50-51 13.243023641843063 30.149462629333023 34.71704104417867 21.89047268464524 52-53 14.15385516571357 28.51851095373215 34.415263352408154 22.912370528146123 54-55 14.801371558939172 27.97594858291833 34.35186710638253 22.87081275175997 56-57 15.184591352087311 26.145616339557833 34.63868567357919 24.031106634775668 58-59 14.27528512363016 26.21472695124214 35.41157164665074 24.098416278476957 60-61 15.583262009808927 25.423885243178855 34.80057480145342 24.192277945558796 62-63 15.175482252740762 25.272823925847614 35.73064250205537 23.821051319356254 64-65 14.431316536154121 25.05711770641191 36.14026520807109 24.37130054936288 66-67 14.59026890208032 23.99745805805424 36.74545492032056 24.666818119544878 68-69 14.6983318122676 23.63362305524552 37.10253164947246 24.565513483014424 70-71 14.520542560450153 22.85348890848758 36.79966179090886 25.82630674015341 72-73 14.946537247368882 22.789893328923238 36.41430828793134 25.84926113577654 74-75 15.046849178235139 22.009684156126646 36.558422149759245 26.38504451587897 76-77 14.102452810170046 21.711932251126093 35.921756995283424 28.26385794342043 78-79 14.83117905249672 21.612442205198334 34.71276426812779 28.843614474177155 80-81 14.302957756195056 21.957660232829795 33.45861735004746 30.28076466092769 82-83 14.475152598803872 21.113065694555768 32.030623805413406 32.381157901226956 84-85 15.315538713259915 20.6101684203874 30.548227512799347 33.526065353553335 86-87 15.162806881261027 20.00158367954649 30.09268571243616 34.742923726756324 88-89 14.998749620936703 19.983292315505512 29.430281830431642 35.58767623312614 90-91 15.684860567286583 19.844896663278494 28.068467561771232 36.4017752076637 92-93 15.736369189939275 20.307935698806283 26.975862296824026 36.97983281443042 94-95 15.014012757504164 20.1720136297858 27.685367552664392 37.12860606004565 96-97 15.462660805307587 20.220610048814695 27.21937330718582 37.097355838691904 98-99 15.515828416672864 21.064953859230098 26.78075707773711 36.638460646359924 100-101 15.186374590869775 20.814638722839433 27.249546802186135 36.74943988410465 102-103 14.661407019763747 22.08227150165796 27.742954921825813 35.51336655675248 104-105 14.517293592297348 22.365602376448194 28.453343710387713 34.66376032086674 106-107 13.813762673936731 22.839882657723955 29.223990592891347 34.12236407544797 108-109 13.489382811183084 23.699337780239365 29.98020564933658 32.83107375924097 110-111 13.624968381297755 24.09710182927076 30.879291787305785 31.3986380021257 112-113 12.773328694496989 24.939062296220136 31.422780038439925 30.86482897084295 114-115 12.954056587526425 24.61424863389557 32.09677910275363 30.334915675824377 116-117 12.855852037375975 25.082670262980443 32.98566612079761 29.07581157884597 118-119 12.69590863884221 26.494373545123196 32.61567438657712 28.194043429457476 120-121 12.606527584611824 27.198872876263568 33.258203310357636 26.936396228766966 122-123 12.193802115791573 28.629590066586847 33.08899212381513 26.08761569380645 124-125 11.370504339262917 30.245522663934292 33.56571231970787 24.818260677094923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17.0 1 10.0 2 16.5 3 87.5 4 181.5 5 356.0 6 812.0 7 1514.5 8 2586.0 9 3826.0 10 4820.5 11 5931.0 12 7135.0 13 8242.5 14 9465.5 15 10953.0 16 12642.5 17 14267.0 18 15948.5 19 17939.0 20 19990.5 21 22264.0 22 25074.0 23 28328.0 24 32436.0 25 37006.0 26 41855.0 27 47699.0 28 54448.0 29 61586.5 30 68561.0 31 75200.5 32 80514.0 33 82570.0 34 81162.0 35 76568.0 36 70164.0 37 63149.5 38 54369.5 39 44709.0 40 36206.0 41 29380.0 42 24942.5 43 25219.0 44 28472.5 45 32908.0 46 43129.0 47 50533.5 48 60783.0 49 82145.5 50 114351.5 51 137909.5 52 139854.5 53 134716.5 54 120721.5 55 100987.0 56 79630.0 57 57787.5 58 39820.5 59 27163.0 60 17219.5 61 11428.0 62 7953.5 63 4194.5 64 1612.5 65 496.0 66 321.0 67 242.5 68 194.5 69 167.5 70 141.0 71 115.5 72 106.0 73 83.5 74 64.0 75 54.5 76 50.5 77 37.0 78 20.0 79 16.5 80 13.5 81 10.5 82 10.0 83 6.0 84 3.5 85 5.0 86 3.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10367429243547518 2 0.0073199983510740555 3 0.006472419594633901 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.4305253177553494E-4 22-23 0.09011303233243272 24-25 0.0 26-27 0.08531650709712366 28-29 0.01907052201990346 30-31 0.00364073602198157 32-33 0.001984104816212178 34-35 0.07788092982471687 36-37 0.0454995686979919 38-39 0.0688657739607625 40-41 0.029780835396738134 42-43 0.015256417615922769 44-45 0.013734628485041585 46-47 0.02702620443830763 48-49 0.016932311975247617 50-51 0.02436788924765442 52-53 0.029279993404296222 54-55 0.02972304593607176 56-57 0.028836940872520686 58-59 0.021979258206777624 60-61 0.02488799439365179 62-63 0.02290388957743961 64-65 0.03802546511847417 66-67 0.02937630917207351 68-69 0.03686967590514669 70-71 0.030223887928513663 72-73 0.060871565235247414 74-75 0.10533092364124458 76-77 0.14270144153883316 78-79 0.042571569357562275 80-81 0.033055571501165996 82-83 0.022691994888329573 84-85 0.022152626588776748 86-87 0.015680206994142847 88-89 0.01602694375814109 90-91 0.012598102425269557 92-93 0.009612313624173563 94-95 0.0052781040741955035 96-97 0.0038141044039806922 98-99 0.007782314036405049 100-101 0.007088840508408559 102-103 0.01044062922705826 104-105 0.006125682830635657 106-107 0.0055863145310828324 108-109 0.0073777878117404294 110-111 0.009650839931284478 112-113 0.00579820922019287 114-115 0.004237893782200769 116-117 0.014601470395037196 118-119 0.018415574799017887 120-121 0.013503470642376086 122-123 0.0056441039917492055 124-125 0.003255472950872409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2595629.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.07405158358209 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10934164127542 17.79224926606843 2 10.130263786176249 4.674924583150487 3 4.093846261583864 2.833848594451223 4 1.951193376587983 1.8008774648373935 5 1.1781804122349058 1.3592697803337113 6 0.7842172450296944 1.0857041498729882 7 0.565994416496621 0.9141849053583734 8 0.42961922156931887 0.7930444863831078 9 0.34937436514241194 0.7255333910949531 >10 2.602676233021304 12.316812111412226 >50 0.3819518422212133 6.207444306700631 >100 0.33996594607382474 15.991037045274577 >500 0.04624591545093979 7.366795808291105 >1k 0.03414572968779067 15.307902830556861 >5k 0.0018233156629402787 3.0489438835662717 >10k+ 0.00116029178550745 7.7814273926477355 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81734 3.1489091854036153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37956 1.4623045127019307 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31399 1.209687516975654 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18397 0.7087684719195232 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12410 0.4781114712464686 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 10836 0.41747106385388666 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10722 0.4130790648432422 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 8676 0.33425424049430796 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 8542 0.32909171534144516 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8457 0.3258169792370173 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 7748 0.2985018274953778 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 7540 0.29048835561630726 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 7194 0.2771582533559303 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7117 0.27419172770838973 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6785 0.2614009937475656 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 6166 0.23755320964590856 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 6164 0.23747615703168676 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 5329 0.2053066905940718 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA 4509 0.1737151187631206 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4425 0.17047890896580367 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 4352 0.1676664885467068 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 4311 0.16608690995515923 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 4266 0.164353226135168 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 4234 0.1631203843076187 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 4224 0.16273512123650954 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4105 0.1581504906903105 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 4063 0.15653238579165205 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 3922 0.1511001764890129 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3913 0.15075343972501462 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 3857 0.14859596652680335 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3713 0.14304817830283142 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 3643 0.14035133680506728 No Hit GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA 3628 0.13977344219840354 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3613 0.1391955475917398 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 3578 0.13784712684285774 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3567 0.13742333746463767 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG 3557 0.13703807439352853 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG 3554 0.13692249547219576 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 3418 0.13168291770511117 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3312 0.12759912915135407 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA 3227 0.12432439304692619 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3178 0.12243660399849131 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3177 0.12239807769138039 No Hit CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA 3167 0.12201281462027122 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA 3062 0.11796755237362504 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT 3042 0.11719702623140672 No Hit GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA 3019 0.11631092116785564 No Hit ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT 3009 0.11592565809674649 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT 3009 0.11592565809674649 No Hit GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC 3004 0.11573302656119192 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 2995 0.11538628979719368 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA 2995 0.11538628979719368 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2970 0.11442313211942076 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA 2969 0.11438460581230986 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG 2916 0.1123427115354313 No Hit GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA 2760 0.10633260762612838 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 2758 0.10625555501190655 No Hit TATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAC 2754 0.10610144978346289 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2727 0.10506123949146814 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.10425218704213893 No Hit CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC 2697 0.10390545027814067 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2650 0.10209471384392763 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA 2645 0.10190208230837303 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG 2638 0.10163239815859663 No Hit GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC 2630 0.10132418770170931 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG 2619 0.10090039832348922 No Hit GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 2608 0.10047660894526914 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.852630711091608E-5 0.0 2 0.0 0.0 0.0 7.705261422183216E-5 0.0 3 0.0 0.0 0.0 7.705261422183216E-5 0.0 4 0.0 0.0 0.0 1.1557892133274825E-4 0.0 5 0.0 0.0 0.0 2.696841497764126E-4 0.0 6 0.0 0.0 0.0 2.696841497764126E-4 0.0 7 0.0 0.0 0.0 3.0821045688732865E-4 0.0 8 0.0 0.0 0.0 3.0821045688732865E-4 0.0 9 0.0 0.0 0.0 4.237893782200769E-4 0.0 10-11 0.0 0.0 0.0 0.0010402102919947341 0.0 12-13 5.7789460666374125E-5 0.0 0.0 0.0019263153555458042 0.0 14-15 1.1557892133274825E-4 0.0 0.0 0.0023308415802104233 0.0 16-17 1.5410522844366432E-4 0.0 0.0 0.0045653673926435556 0.0 18-19 1.926315355545804E-4 0.0 0.0 0.0075704193472950106 0.0 20-21 1.926315355545804E-4 0.0 0.0 0.01594989114391926 0.0 22-23 1.926315355545804E-4 0.0 0.0 0.04842756803842151 0.0 24-25 1.926315355545804E-4 0.0 0.0 0.11991313088272632 0.0 26-27 1.926315355545804E-4 0.0 0.0 0.20499848013718447 0.0 28-29 1.926315355545804E-4 0.0 0.0 0.4305700082715982 0.0 30-31 2.311578426654965E-4 0.0 0.0 1.1065525928397317 0.0 32-33 2.696841497764126E-4 0.0 0.0 2.2802758021273455 0.0 34-35 3.4673676399824476E-4 0.0 0.0 3.0471034188630193 0.0 36-37 3.4673676399824476E-4 0.0 0.0 3.8657103923557643 0.0 38-39 3.4673676399824476E-4 0.0 0.0 4.835899121176409 0.0 40-41 3.4673676399824476E-4 0.0 0.0 6.171105346719427 0.0 42-43 3.4673676399824476E-4 0.0 0.0 7.3240243501671465 0.0 44-45 3.4673676399824476E-4 0.0 0.0 8.261407928482846 0.0 46-47 3.4673676399824476E-4 0.0 0.0 9.121469208426937 0.0 48-49 3.4673676399824476E-4 0.0 0.0 9.88321135262397 0.0 50-51 3.4673676399824476E-4 0.0 0.0 10.639347919136362 0.0 52-53 3.4673676399824476E-4 0.0 0.0 11.52483656177366 0.0 54-55 3.4673676399824476E-4 0.0 0.0 12.312680279038338 0.0 56-57 3.4673676399824476E-4 0.0 0.0 12.974812656200097 0.0 58-59 3.4673676399824476E-4 0.0 0.0 13.709856069569264 0.0 60-61 3.4673676399824476E-4 0.0 0.0 14.390731495140484 0.0 62-63 3.4673676399824476E-4 0.0 0.0 15.059259239282657 0.0 64-65 3.659999175537028E-4 0.0 0.0 15.759031818491781 0.0 66-67 3.852630711091608E-4 0.0 0.0 16.387665571620598 0.0 68-69 3.852630711091608E-4 0.0 0.0 16.99651606604796 0.0 70-71 3.852630711091608E-4 0.0 0.0 17.690702330726 0.0 72-73 3.852630711091608E-4 0.0 0.0 18.33748197450406 0.0 74-75 3.852630711091608E-4 0.0 0.0 18.92165636922688 0.0 76-77 3.852630711091608E-4 0.0 0.0 19.53794244092665 0.0 78-79 3.852630711091608E-4 0.0 0.0 20.13313535948319 0.0 80-81 3.852630711091608E-4 0.0 0.0 20.718773753876228 0.0 82-83 3.852630711091608E-4 0.0 0.0 21.39115412872949 0.0 84-85 3.852630711091608E-4 0.0 0.0 21.971803366351665 0.0 86-87 3.852630711091608E-4 0.0 0.0 22.51918513778356 0.0 88-89 3.852630711091608E-4 0.0 0.0 23.056280385216837 0.0 90-91 3.852630711091608E-4 0.0 0.0 23.577926583498645 0.0 92-93 3.852630711091608E-4 0.0 0.0 24.064436789695293 0.0 94-95 3.852630711091608E-4 0.0 0.0 24.55137078527016 0.0 96-97 3.852630711091608E-4 0.0 0.0 25.03512636050838 0.0 98-99 3.852630711091608E-4 0.0 0.0 25.514355094661063 0.0 100-101 3.852630711091608E-4 0.0 0.0 26.036174661324864 0.0 102-103 3.852630711091608E-4 0.0 0.0 26.505925923928267 0.0 104-105 3.852630711091608E-4 0.0 0.0 26.933933932776988 0.0 106-107 3.852630711091608E-4 0.0 0.0 27.37076446595411 0.0 108-109 3.852630711091608E-4 0.0 0.0 27.859682566345192 0.0 110-111 3.852630711091608E-4 0.0 0.0 28.312655622201788 0.0 112-113 3.852630711091608E-4 0.0 0.0 28.78510372630295 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAAGTA 140 0.0 80.70179 2 ATAGTAC 1000 0.0 80.26931 4 CGCATAG 995 0.0 79.58637 1 GTATAAC 490 0.0 76.55166 1 AGTGTAC 995 0.0 75.89792 3 CATAGTA 1080 0.0 74.32916 3 GCATAGT 1085 0.0 73.439995 2 TAGGACC 465 0.0 72.88491 4 GTACAAC 485 0.0 71.2027 1 ATAACGC 535 0.0 71.133705 3 TACAACG 485 0.0 71.11216 2 GACCGTT 490 0.0 67.95285 7 TAGTACT 1185 0.0 67.737816 5 GTGTACT 1195 0.0 64.18559 4 TCACGCA 505 0.0 62.402245 4 AGATTAC 1370 0.0 61.63471 2 CTGAGTA 395 0.0 60.21139 4 TTAGGAC 570 0.0 59.463333 3 ACTCAGA 235 0.0 58.198154 3 TGAGTAC 430 0.0 56.693222 5 >>END_MODULE