FastQCFastQC Report
Fri 10 Feb 2017
SRR3557242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475754
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT738262.9819602432228725No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA553522.235763327051072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505082.04010576171946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477591.9290688816417139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272611.1011190934155817No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG240830.9727541589350153No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166870.674016885360985No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG148770.6009078446404611No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148500.5998172677899338No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT141490.5715026614114326No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122180.4935062207311389No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121310.48999213976832917No Hit
GTGATAAGGTACCCGGGGATCCTCTAGAGTCGACCTGCCCATGTACTCTG118660.4792883299390812No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT112080.4527105681743825No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108340.43760405920782114No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA98920.39955504464498487No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89910.36316209122554177No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT88910.3591229177050709No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT86090.34773244837734285No Hit
CCTTATCACATCGCGCGCTGGAAGATGCGATGTGATAAGGTACCCGGGGA85260.3443799343553519No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA82390.33278750635160037No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63250.2554777251697867No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62350.2518424690013628No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT61380.24792447068650603No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57200.2310407253709375No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54800.22134670892180724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC53760.21714596846051748No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA53660.2167420511084704No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50210.2028069024628457No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50080.20228180990518443No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA49670.20062574876179134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGGTCGACTC49620.20042379008576783No Hit
GAAGATGGGAGGGGGAAACACAGGTGGAAAAAAAAAAAAAAAAAAAAAAA48460.19573834880202154No Hit
CTCTAGAGTCGACCTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCA47970.19375915377699077No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG46850.18923527943406332No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT45490.18374200344622285RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA43500.17570404814048568No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA43090.17404798699709262No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.17158409114960532No Hit
GTATCAACGCAGAGTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTA41720.16851431927404742No Hit
AGATGGGAGGGGGAAACACAGGTGGAAAAAAAAAAAAAAAAAAAAAAAAA40490.16354613584386818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC39730.16047636396831028No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA39720.16043597223310554No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.1601128383514679No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.15950696232339723No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA38010.15352898551310024No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA37080.14977255413906226No Hit
ACTCTAGAGGATCCCCGGGTACCTTATCACATCGCGCGCTGGAAGATGCG36480.14734905002677973No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT36390.1469855244099373No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34170.13801855919449185No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA32500.1312731394153054No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT31510.12727435763003916No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31300.12642613119074028No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT30890.1247700700473472No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT30390.12275048328711173RNA PCR Primer, Index 39 (96% over 25bp)
ACTCTAGAGGATCCCCGGGTACCTTATCACCTGTCTCTTATACACATCTC30150.1217810816421987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.12149833949576573No Hit
GTGATAAGGTACCCGGGGATCCTCTAGAGTCTGTCTCTTATACACATCTC29800.12036737091003387No Hit
TATCAACGCAGAGTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTAC29280.11826700067938899No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT28410.11475291971657928No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT28400.11471252798137457No Hit
GTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTACCTTATCACATCG27690.11184471478184019No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT26880.10857298423025874No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT26460.10687653135166095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT26350.10643222226440914No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC26130.10554360408990555No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT25770.10408950162253601No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25320.10227187353832408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC25300.10219109006791467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAA22600.049.9923552
GTGATGA23650.048.3244971
AAGATGG53150.044.976042
TAAGGTA99300.040.5372285
ATAAGGT99100.040.379044
AAGGTAC100450.039.303646
AGGTACC99850.039.301627
TGATAAG103650.039.069362
GTGATAA103950.038.9959831
GATGAAG29350.038.6960073
GGTACCC103000.037.868778
GTACCCG101600.037.8638849
GATAAGG107900.037.3636363
TGAAGAT30950.036.6933445
ACGCGGA1304.231879E-836.5899057
ATGAAGA31550.036.560914
GTATCCA7200.036.3358044
GGTAAGT2800.036.0998578
CAGGGTA3850.035.5602151
GAACAGT8400.035.4313741
Produced by FastQC (version 0.11.5)