FastQCFastQC Report
Fri 10 Feb 2017
SRR3557217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182755
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330650.7905076917008048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150240.35918909905074525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127950.30589886330899135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56270.134528558330574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG308800.039.943756
GTATCAA304600.039.4221951
ACGCAGA350300.035.2625277
CGCAGAG356600.034.6895568
ATCAACG351200.034.0893063
TCAACGC356750.033.992214
ACATGGG315100.033.580323
CAACGCA362200.033.464325
TATCAAC357100.033.45952
GTACATG324350.033.4443171
TACATGG328150.033.005722
GCAGAGT380650.032.466599
CATGGGG111450.029.1756464
CGCATAG7200.028.9257811
CATGGGT45950.028.4610164
AGAGTAC366450.026.39283610-11
CATGGGC87600.025.854384
TAACGCA6750.025.539214
CATGGGA167100.024.0126764
AGTACTT223950.023.94245712-13
Produced by FastQC (version 0.11.5)