FastQCFastQC Report
Fri 10 Feb 2017
SRR3557216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647622
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89611.3836775155877965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.6990188721198477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.60714429095985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.29677805880590835No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16110.24875621890547264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.2442782981430526No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11760.18158740746917185No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9860.15224930592228184No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.14946990682836592No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA9610.14838902940295418No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.12908764680631604No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG8100.12507295922621528No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT8070.12460972604389597No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7290.11256566330359376No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7120.10994067527045097No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.10700686511576198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG1400.055.207882
CGCATAG1200.054.5548481
TCACGCA1200.054.5000844
AGATTAC2600.048.0210532
ATGGGTG6900.045.6680535
CATGGGT12850.045.342844
GTATAAC2300.043.9888841
ACAACGC1950.042.685383
AAACGCA2250.042.2788545
GTACAAC1950.039.676251
GTATAAA2550.039.676251
GATTACT3150.039.6364253
TTAGAGC2000.038.6455154
GTACCCG1401.906301E-938.2178889
GTTAGAG2350.037.9497683
TATCACG1601.70985E-1037.159152
TAACGCA2250.036.9939964
ATAGTAC2001.8189894E-1235.6727834
CATGGGC23050.035.3374634
CATGGGG24600.035.2860834
Produced by FastQC (version 0.11.5)