FastQCFastQC Report
Fri 10 Feb 2017
SRR3557215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437808
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487361.0981998319891262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248340.5596005956093639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182090.4103151826307042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88180.1987017013805014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA346250.051.4641381
AACGCAG409100.044.326656
TATCAAC430000.041.385222
ATCAACG432450.041.2327583
TCAACGC442200.040.780634
CAACGCA448500.040.075265
ACGCAGA466650.038.7963457
CGCAGAG470850.038.4376568
GCAGAGT499100.036.2858289
GTACATG406550.034.37691
TACATGG410950.034.018122
GGTATCA193350.033.910571
ACATGGG406450.033.8386733
CATGGGG155450.030.244254
AGAGTAC487150.029.7222310-11
CGCATAG6900.028.4552571
CATGGGT62850.028.3708954
CATGGGA181950.026.8193444
AGTACTT291850.026.64899612-13
GGACCGT11300.026.2992736
Produced by FastQC (version 0.11.5)