FastQCFastQC Report
Fri 10 Feb 2017
SRR3557213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928595
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346481.1830929165692081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167480.5718783239061734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130540.44574275377783545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65560.2238616128211651No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49640.16950107474744713No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39550.13504769351856435No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA37710.12876481725878794No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.10868693008080667No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30090.10274551448732241No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.10264307628743476No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29680.1013455257555244No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC29600.10107235722249064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5200.040.0382961
AACGCAG354800.037.9436766
GTATCAA347400.037.5679661
TAACGCA7400.035.3407484
TACAACG4750.033.7850842
ACGCAGA397950.033.6651237
CGCAGAG399250.033.5555048
ACATGGG350100.032.8506283
CATGGGT49100.032.8051954
TCAACGC405500.032.745164
ATCAACG398150.032.648023
TATCAAC398900.032.571742
CATGGGG89100.032.286614
CAACGCA410450.032.277855
GTACATG365200.032.2022931
TACATGG364900.032.0883332
GTATAAC10450.031.8773781
GCAGAGT422000.031.746539
CATGGGC80250.029.6998424
TATCACG5050.029.4241162