FastQCFastQC Report
Fri 10 Feb 2017
SRR3557210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5105192
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427820.8380096184433417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223150.4371040305633951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188920.37005464241109837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90530.17732927576475085No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82320.16124760831717985No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65060.12743888966369923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60350.11821298787587226No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56210.11010359649548929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG12200.053.15741
TAACGCA13700.039.5173764
TACAACG10700.037.8091282
ACAACGC11800.032.7719963
TATCACG6900.031.902462
AACGCAG605800.031.0527366
AGATTAC27100.030.734792
TAGTACT22150.030.6195225
GTATAAC20050.029.674451
ATAACGC20200.029.4523643
GTATCAA613800.029.322131
CATGGGG137450.029.2596574
TCACGCA8850.028.9062984
CGCAGAG672200.028.0295968
ACGCAGA670650.028.0056697
ACATGGG546350.027.7133083
ACGGTAC9300.027.5078743
GCATAGT24150.026.8522642
GATTACT30900.026.7625733
GCAGAGT705600.026.7027999