FastQCFastQC Report
Fri 10 Feb 2017
SRR3557206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8853297
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579980.6551005800437961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347170.392136398451334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270170.3051631499541922No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA127530.1440480309200064No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125810.14210525186266768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116750.13187177613040657No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA98680.11146130080127212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG19650.056.0176621
ATAGTAC30550.036.6115654
TAGTACT30950.035.946175
AGATTAC42750.035.3483622
TAACGCA18850.034.717864
TATCACG13150.033.9318242
TACAACG17400.032.4822432
GATTACT47650.031.5885183
AACGCAG936250.030.0249066
ACATGGG923350.029.1363983
CATGGGG240750.028.5669044
GTACATG992000.028.5023441
GTATCAA935200.028.420211
TACATGG995600.028.3067572
GCATAGT39550.028.2802332
CATGGGC236400.028.2117354
GTATAAC24800.028.070491
ACAACGC20450.027.6377033
ACGCAGA1037850.027.0397687
CGCAGAG1049000.026.8542948
Produced by FastQC (version 0.11.5)