##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557202.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1469138 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.114698551123176 33.0 32.0 34.0 30.0 34.0 2 32.14400280981092 33.0 32.0 34.0 30.0 34.0 3 32.1893947335104 33.0 32.0 34.0 30.0 34.0 4 32.07676950701704 33.0 32.0 34.0 30.0 34.0 5 32.1064025299189 33.0 33.0 34.0 30.0 34.0 6 35.27967080015628 38.0 35.0 38.0 29.0 38.0 7 35.67108739954994 38.0 36.0 38.0 30.0 38.0 8 35.972235419681475 38.0 37.0 38.0 31.0 38.0 9 36.1166459515716 38.0 37.0 38.0 33.0 38.0 10-11 36.05121336457161 38.0 37.0 38.0 31.5 38.0 12-13 36.09990177913852 38.0 37.0 38.0 32.0 38.0 14-15 36.21426850302694 38.0 37.0 38.0 33.0 38.0 16-17 36.19888295041038 38.0 37.0 38.0 33.0 38.0 18-19 36.23511099706086 38.0 37.0 38.0 33.0 38.0 20-21 36.11944725410411 38.0 37.0 38.0 32.5 38.0 22-23 36.116109582626 38.0 37.0 38.0 32.5 38.0 24-25 36.08711741170673 38.0 37.0 38.0 32.0 38.0 26-27 35.96220436745901 38.0 37.0 38.0 31.5 38.0 28-29 35.86055564555542 38.0 37.0 38.0 31.0 38.0 30-31 35.59659201518169 38.0 37.0 38.0 30.0 38.0 32-33 35.33517579696394 38.0 36.0 38.0 28.0 38.0 34-35 34.965076800137226 38.0 36.0 38.0 26.0 38.0 36-37 34.578021601782815 38.0 35.5 38.0 24.0 38.0 38-39 34.258941297549995 38.0 35.0 38.0 16.0 38.0 40-41 33.86424862742642 38.0 34.0 38.0 15.0 38.0 42-43 33.39435846053945 38.0 33.5 38.0 15.0 38.0 44-45 32.77374657792528 38.0 31.5 38.0 15.0 38.0 46-47 32.134190253059955 38.0 27.5 38.0 15.0 38.0 48-49 31.509947329658615 38.0 25.5 38.0 15.0 38.0 50-51 31.02156842992285 38.0 24.0 38.0 15.0 38.0 52-53 30.634800474836265 38.0 20.0 38.0 15.0 38.0 54-55 30.297392076169835 37.0 16.0 38.0 15.0 38.0 56-57 30.037116322632727 37.0 15.0 38.0 15.0 38.0 58-59 29.842884739214423 37.0 15.0 38.0 15.0 38.0 60-61 29.658806048172465 37.0 15.0 38.0 15.0 38.0 62-63 29.5089695454069 37.0 15.0 38.0 15.0 38.0 64-65 29.321273086667148 36.0 15.0 38.0 14.5 38.0 66-67 29.153570324911612 36.0 15.0 38.0 14.0 38.0 68-69 28.955374852464505 36.0 15.0 38.0 14.0 38.0 70-71 28.735082068532705 35.5 15.0 38.0 14.0 38.0 72-73 28.482428471661613 35.0 15.0 38.0 14.0 38.0 74-75 28.28191735561942 35.0 14.0 38.0 14.0 38.0 76-77 28.094489081352467 35.0 14.0 38.0 14.0 38.0 78-79 27.917785122976873 34.0 14.0 38.0 14.0 38.0 80-81 27.77602478460158 34.0 14.0 38.0 13.5 38.0 82-83 27.601054836237303 34.0 14.0 38.0 13.0 38.0 84-85 27.468634328429324 34.0 14.0 38.0 13.0 38.0 86-87 27.34770967737544 34.0 14.0 38.0 13.0 38.0 88-89 27.20963653516552 33.5 14.0 38.0 13.0 38.0 90-91 27.048315406721493 33.0 14.0 38.0 13.0 38.0 92-93 26.972394356418526 33.0 14.0 38.0 13.0 38.0 94-95 26.903733686011797 32.0 14.0 38.0 13.0 38.0 96-97 26.80327069342703 31.0 14.0 38.0 13.0 38.0 98-99 26.69688313827564 31.0 14.0 38.0 13.0 38.0 100-101 26.54990477409202 30.0 14.0 38.0 13.0 38.0 102-103 26.405891754212334 28.5 14.0 38.0 13.0 38.0 104-105 26.280693168374924 27.5 14.0 38.0 13.0 38.0 106-107 26.161540644922397 26.5 14.0 38.0 13.0 38.0 108-109 25.943081249004518 24.5 14.0 38.0 13.0 38.0 110-111 25.84524700879019 24.0 14.0 38.0 13.0 38.0 112-113 25.693038707051347 24.0 14.0 38.0 13.0 38.0 114-115 25.58882589654614 24.0 14.0 38.0 13.0 38.0 116-117 25.344306321121636 23.0 14.0 38.0 13.0 38.0 118-119 25.158964304238268 23.0 14.0 38.0 13.0 38.0 120-121 25.06405899241596 23.0 14.0 38.0 13.0 38.0 122-123 24.885040751787784 23.0 14.0 38.0 13.0 38.0 124-125 24.063648207316128 22.0 14.0 36.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08327682386448032 1101 2 0.14923878504584565 1101 3 0.004188584218596247 1101 4 0.1450421860982658 1101 5 0.04980189930062551 1101 6 0.18110178973795854 1101 7 0.24339192747492433 1101 8 0.028843174845697206 1101 9 0.3254235155801197 1101 10-11 0.2562859026303457 1101 12-13 0.1235858743276097 1101 14-15 0.15116867878585083 1101 16-17 -0.2057998043630178 1101 18-19 0.10083059260517047 1101 20-21 0.1437650924352738 1101 22-23 0.08982129992379129 1101 24-25 0.19429244221775122 1101 26-27 -0.0019891568347318866 1101 28-29 0.08460387463413355 1101 30-31 -0.20800748264696267 1101 32-33 -0.09452943357069898 1101 34-35 -0.47507306312429876 1101 36-37 -0.4369035765720426 1101 38-39 -0.11841355369968909 1101 40-41 0.05066352202300095 1101 42-43 0.11566493754923357 1101 44-45 -0.014015778986632199 1101 46-47 -0.1095144934647152 1101 48-49 -0.25712622935770213 1101 50-51 -0.06851606613126648 1101 52-53 -0.08870563755810679 1101 54-55 0.02681680579109269 1101 56-57 0.10492625488950225 1101 58-59 -0.15308169322323195 1101 60-61 -0.20520991973004143 1101 62-63 -0.05352993769092507 1101 64-65 0.02475245527345038 1101 66-67 -0.054080095374928305 1101 68-69 0.01656560206434321 1101 70-71 -0.09982297767973591 1101 72-73 -0.3739159690981424 1101 74-75 -0.20661383625329321 1101 76-77 -0.20589350444431886 1101 78-79 -0.21429666297773053 1101 80-81 -0.17003829987725894 1101 82-83 -0.2533417851337951 1101 84-85 -0.11878620731620515 1101 86-87 0.13568821541846177 1101 88-89 -0.03595564181240363 1101 90-91 -0.1283966512525403 1101 92-93 -0.0656942980315911 1101 94-95 -0.18992014802497081 1101 96-97 -0.11373747842164761 1101 98-99 0.21462271530756283 1101 100-101 0.06815859860058637 1101 102-103 0.01346833184380003 1101 104-105 0.1444508980460526 1101 106-107 0.03439804072642616 1101 108-109 -0.22734025493525678 1101 110-111 -0.36714815855902927 1101 112-113 -0.3119918636168748 1101 114-115 -0.018696374646435032 1101 116-117 -0.23758926398614832 1101 118-119 -0.3020524289109545 1101 120-121 -0.04472350756929444 1101 122-123 -0.15849718563400828 1101 124-125 -0.47036283815822344 1102 1 -0.005097262936168079 1102 2 -0.018336018789646857 1102 3 0.058974814765470285 1102 4 -0.0012938987722463935 1102 5 0.08688232044398347 1102 6 0.21423164227359592 1102 7 0.34933199271409165 1102 8 0.21098102983869182 1102 9 0.20323738451953943 1102 10-11 0.06901648045315767 1102 12-13 0.23674855275535123 1102 14-15 0.10536349312012305 1102 16-17 -0.012001880738232273 1102 18-19 0.14680403389106544 1102 20-21 0.18838286105400215 1102 22-23 0.21072534149714528 1102 24-25 0.007406523654381658 1102 26-27 -0.21955467681107876 1102 28-29 0.012481584432158854 1102 30-31 -0.07684787133119642 1102 32-33 -0.051253812984832336 1102 34-35 -0.10148621548732706 1102 36-37 -0.21507527524318704 1102 38-39 -0.09616828101784591 1102 40-41 0.04825109403021344 1102 42-43 0.09147349291198026 1102 44-45 -0.09185119564853039 1102 46-47 -0.11766893103477116 1102 48-49 0.09461787539173727 1102 50-51 0.23648323471390853 1102 52-53 0.04517746833978009 1102 54-55 0.03908869220250111 1102 56-57 -0.0913308598704603 1102 58-59 -0.2504611234005907 1102 60-61 -0.02770184506489315 1102 62-63 0.07832814075653971 1102 64-65 0.21038883516438034 1102 66-67 0.19564287885261678 1102 68-69 0.24840373740696364 1102 70-71 0.05979315193669521 1102 72-73 0.20307885715615015 1102 74-75 0.06298576580153181 1102 76-77 0.08221696660264755 1102 78-79 0.012974501355746071 1102 80-81 -0.11889936520582722 1102 82-83 0.11290485376534676 1102 84-85 0.11178221701805313 1102 86-87 0.046848789504803534 1102 88-89 -0.08084321542423467 1102 90-91 0.08065683065310125 1102 92-93 0.17784425061468667 1102 94-95 -0.008707698942924225 1102 96-97 0.09574581177803765 1102 98-99 0.11398109748622431 1102 100-101 0.0654809502364948 1102 102-103 -0.04086218729584701 1102 104-105 0.13402821883891747 1102 106-107 0.045396777663118115 1102 108-109 0.17474437259860665 1102 110-111 -0.11020555114707165 1102 112-113 -0.28707077641966094 1102 114-115 -0.014078743566368956 1102 116-117 -0.08453349893884621 1102 118-119 0.2854143612348601 1102 120-121 0.13303053205559223 1102 122-123 0.007748536340766776 1102 124-125 -0.22912874966127106 1103 1 0.21750018776117486 1103 2 0.13484079250707026 1103 3 0.021685741336362696 1103 4 0.14862053693851607 1103 5 0.17050105400326032 1103 6 0.19298313992755567 1103 7 0.2692464953591056 1103 8 0.21518673219098616 1103 9 0.10633800232270829 1103 10-11 0.06503411539819126 1103 12-13 0.28823505100056224 1103 14-15 0.05882655991382535 1103 16-17 0.1373014452248995 1103 18-19 -0.14371740626636154 1103 20-21 0.08687795588868141 1103 22-23 0.08381844035234565 1103 24-25 -0.09222779219944499 1103 26-27 -0.09743089752455347 1103 28-29 0.07925006553773528 1103 30-31 0.0948860159202809 1103 32-33 0.15848891405058652 1103 34-35 0.1373443538331287 1103 36-37 0.14851137449655027 1103 38-39 0.15421759496189935 1103 40-41 0.20654034371952434 1103 42-43 0.3930230209139296 1103 44-45 0.2712381325769826 1103 46-47 0.3155904771325453 1103 48-49 0.5006807802045294 1103 50-51 0.4079304202400458 1103 52-53 0.48034427965880866 1103 54-55 0.31181900739590773 1103 56-57 0.16036737373454812 1103 58-59 0.040542020278252267 1103 60-61 0.3336119404911422 1103 62-63 0.20859134855839656 1103 64-65 0.3361277335604136 1103 66-67 0.1036441996885813 1103 68-69 0.11377708552532795 1103 70-71 0.07442364138227475 1103 72-73 0.12256855039645842 1103 74-75 0.19647074240801388 1103 76-77 0.20588112620960786 1103 78-79 0.24698892791883154 1103 80-81 0.2703313594021033 1103 82-83 0.1484362336596341 1103 84-85 0.2056521548361303 1103 86-87 0.233557208272277 1103 88-89 0.2290307027508689 1103 90-91 0.08132461140325731 1103 92-93 0.32389144818898785 1103 94-95 0.015596575255482747 1103 96-97 0.1940807106292226 1103 98-99 0.24026260957566592 1103 100-101 0.045808080251823924 1103 102-103 0.019862130588311544 1103 104-105 0.34137336086224934 1103 106-107 0.18827329958073946 1103 108-109 0.12905772754426437 1103 110-111 -0.16054435003417566 1103 112-113 0.006966720149954142 1103 114-115 -0.20678224043495774 1103 116-117 -0.25310451953005497 1103 118-119 -0.21920990332501233 1103 120-121 -0.1518006039748947 1103 122-123 0.1633746951400603 1103 124-125 0.19718957626839284 1104 1 0.17529738589326627 1104 2 0.27667401465517116 1104 3 0.18156121387360713 1104 4 0.2631408838364493 1104 5 0.20966938844844663 1104 6 0.3226887214263314 1104 7 0.3152918589348275 1104 8 0.2190679863604359 1104 9 0.3435272395919995 1104 10-11 0.2419585094386676 1104 12-13 0.21431377014665998 1104 14-15 0.14598110738991465 1104 16-17 0.19941952394404439 1104 18-19 0.07522766822774685 1104 20-21 0.27908631701832576 1104 22-23 0.21303080415043496 1104 24-25 0.2541133241003095 1104 26-27 0.3229982774810125 1104 28-29 0.2893032120798722 1104 30-31 0.29475970949377484 1104 32-33 0.24216647687024562 1104 34-35 0.27993630402214364 1104 36-37 0.3953550480567003 1104 38-39 0.2719387423935231 1104 40-41 0.3871474151004435 1104 42-43 0.2421591116410724 1104 44-45 0.22454122352650074 1104 46-47 0.14462761300089966 1104 48-49 0.06835812065371982 1104 50-51 0.06364934396697564 1104 52-53 0.1029400101458009 1104 54-55 -0.03122568906839973 1104 56-57 -0.23775114972553268 1104 58-59 -0.1393663630884383 1104 60-61 -0.10736070794114383 1104 62-63 -0.26386806883097336 1104 64-65 -0.2856256575892431 1104 66-67 -0.21419547120312288 1104 68-69 -0.3926007219018466 1104 70-71 -0.40243092833086536 1104 72-73 -0.521825267704493 1104 74-75 -0.40385258425420645 1104 76-77 -0.3586392206883211 1104 78-79 -0.3111191441292043 1104 80-81 -0.5133519850497521 1104 82-83 -0.2210438641833683 1104 84-85 -0.11445858565753397 1104 86-87 -0.3411712773848272 1104 88-89 -0.35925904597050007 1104 90-91 -0.07209233322649666 1104 92-93 -0.14363512508208132 1104 94-95 -0.14768094309008362 1104 96-97 -0.2582508437403561 1104 98-99 -0.21202224699538164 1104 100-101 -0.2512604098526907 1104 102-103 -0.21975739353888102 1104 104-105 -0.10537980791693613 1104 106-107 -0.19295996704145324 1104 108-109 -0.24499252818177553 1104 110-111 -0.3463371007568803 1104 112-113 -0.41051893695478014 1104 114-115 -0.3233808617380518 1104 116-117 -0.435523465494029 1104 118-119 -0.20752878257784602 1104 120-121 -0.1641043620358218 1104 122-123 -0.403015120292455 1104 124-125 -0.29533610752080364 1105 1 0.1290970005368095 1105 2 0.19938178601033485 1105 3 0.0584012909449001 1105 4 0.06273112147292892 1105 5 0.21330458433797617 1105 6 0.08918454673140275 1105 7 0.3064633425571799 1105 8 0.12120414564110149 1105 9 0.1715041182047159 1105 10-11 0.17898548439049478 1105 12-13 0.13752764297903042 1105 14-15 0.07838059358130778 1105 16-17 0.021548618356383997 1105 18-19 -0.038565523808223645 1105 20-21 0.13866178265737972 1105 22-23 0.18528000132447886 1105 24-25 -0.004766573137963803 1105 26-27 -0.019178986488164185 1105 28-29 -0.00255870185718976 1105 30-31 0.2907828308810636 1105 32-33 0.3048909470051129 1105 34-35 0.32424202014288994 1105 36-37 0.24253809237269053 1105 38-39 0.250710739824477 1105 40-41 0.34225981073306855 1105 42-43 0.4581096575627157 1105 44-45 0.4721815575020969 1105 46-47 0.43038226939139435 1105 48-49 0.4816882426832656 1105 50-51 0.213034058160936 1105 52-53 0.38839087719782484 1105 54-55 0.45832601292260833 1105 56-57 0.3615160307497405 1105 58-59 0.4184936240663468 1105 60-61 0.32101051877690523 1105 62-63 0.23141131459869868 1105 64-65 0.2644867380433844 1105 66-67 0.09879425262474939 1105 68-69 0.3360832858052838 1105 70-71 0.41845218021120445 1105 72-73 0.20589406434431368 1105 74-75 0.18065223270147612 1105 76-77 0.19318351534250766 1105 78-79 0.1507125835522345 1105 80-81 0.05424210615127478 1105 82-83 -7.407171056570405E-4 1105 84-85 0.10858444581324278 1105 86-87 0.21918453507503344 1105 88-89 0.05805566180085364 1105 90-91 0.2625871786553269 1105 92-93 0.019259544153630515 1105 94-95 0.02834924316553611 1105 96-97 0.1922533309545713 1105 98-99 0.14309978254861022 1105 100-101 -0.06334968409803565 1105 102-103 -0.0015884789595652649 1105 104-105 -0.05780819584243346 1105 106-107 0.07087176063998513 1105 108-109 0.2691526227495018 1105 110-111 0.044677671240549444 1105 112-113 0.08612396606384465 1105 114-115 0.07993577281557762 1105 116-117 -0.28550548219280714 1105 118-119 0.062265693979632886 1105 120-121 -0.1812976825239403 1105 122-123 -0.1461397188794784 1105 124-125 -0.3103470259536856 1106 1 0.175202147798025 1106 2 0.11955174953290282 1106 3 0.20104641335881013 1106 4 -0.03675358205801871 1106 5 0.24668354546260218 1106 6 0.3446629814005888 1106 7 0.23497525661822039 1106 8 0.2822417315341781 1106 9 0.2737202897850466 1106 10-11 0.33643856091871527 1106 12-13 0.28211814595103846 1106 14-15 0.2156799490887522 1106 16-17 0.16416212368664418 1106 18-19 0.19796125896133532 1106 20-21 0.3743231252551311 1106 22-23 0.21092269604232428 1106 24-25 0.29525103323802426 1106 26-27 0.18468424916698467 1106 28-29 0.12679484657150653 1106 30-31 0.17762714836121063 1106 32-33 0.21344318214694624 1106 34-35 0.16651802860387477 1106 36-37 0.15951721021886556 1106 38-39 0.2584612649160434 1106 40-41 0.09044213839517568 1106 42-43 0.0221912866732481 1106 44-45 0.22008305106832893 1106 46-47 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0.6485012750589654 2206 72-73 0.5230529494957565 2206 74-75 0.5727487967878524 2206 76-77 0.5775892789978059 2206 78-79 0.5497270580051357 2206 80-81 0.42422240289751656 2206 82-83 0.5955838822070874 2206 84-85 0.6958502655665697 2206 86-87 0.5325424702611237 2206 88-89 0.6390403890577474 2206 90-91 0.3843445782612598 2206 92-93 0.38870825219349925 2206 94-95 0.3431985296029474 2206 96-97 0.44502285368588446 2206 98-99 0.489688361918617 2206 100-101 0.5196579831165486 2206 102-103 0.504260811106441 2206 104-105 0.37954235153064886 2206 106-107 0.39173180948083086 2206 108-109 0.4566873211590945 2206 110-111 0.47016635184826683 2206 112-113 0.21099832607095692 2206 114-115 0.34977419162039425 2206 116-117 0.3702028370797308 2206 118-119 0.5479200560913284 2206 120-121 0.3699634345743448 2206 122-123 0.18505455955688532 2206 124-125 0.15034625280562608 2207 1 0.10613658187316588 2207 2 0.08505089907728092 2207 3 0.0993417833878496 2207 4 -0.6795274578720978 2207 5 0.2370730735405715 2207 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-0.06783731294179418 2313 80-81 -0.1688484085118418 2313 82-83 -0.2594787711933826 2313 84-85 -0.16110236248156795 2313 86-87 -0.05695382387981951 2313 88-89 -0.08828193581313215 2313 90-91 -0.19144569517213483 2313 92-93 -0.08828605498193909 2313 94-95 0.16298143173109025 2313 96-97 0.129510243527168 2313 98-99 0.18601065715340326 2313 100-101 0.07397421103429025 2313 102-103 0.2282726493795728 2313 104-105 0.049245370909961395 2313 106-107 0.3365850973362292 2313 108-109 0.27148529728317783 2313 110-111 0.28838392928603795 2313 112-113 0.43513342356024154 2313 114-115 0.5083044029257557 2313 116-117 0.422482256966628 2313 118-119 0.4755756523291623 2313 120-121 0.26988705427319104 2313 122-123 0.4320134920108387 2313 124-125 0.4519400012059478 2314 1 -0.3336777030747058 2314 2 -0.28329751559394367 2314 3 -0.22631541720586767 2314 4 -0.3242398990817037 2314 5 -0.08340297216840042 2314 6 -0.06359123112408582 2314 7 -0.42640920868083043 2314 8 -0.6113305901520221 2314 9 -0.5140765563747891 2314 10-11 -0.5344958203359482 2314 12-13 -0.4182413899956856 2314 14-15 -0.37091806224235313 2314 16-17 -0.4062210454509696 2314 18-19 -0.4752159853902853 2314 20-21 -0.2294059724241464 2314 22-23 -0.27877465255182443 2314 24-25 -0.4858914208581737 2314 26-27 -0.39388191231733316 2314 28-29 -0.3623824344207165 2314 30-31 -0.2842533391420474 2314 32-33 -0.23868998813568965 2314 34-35 0.04982479749782698 2314 36-37 0.03252705280166168 2314 38-39 -0.05196161347836181 2314 40-41 0.13885560015383192 2314 42-43 -0.014584660600917232 2314 44-45 -0.09263078121846036 2314 46-47 -9.477132149982026E-4 2314 48-49 -0.259109257756716 2314 50-51 -0.32791886481119903 2314 52-53 -0.3121809862954805 2314 54-55 -0.24277017305416493 2314 56-57 -0.11243560998650892 2314 58-59 0.0605292478249666 2314 60-61 -0.1858589285817125 2314 62-63 -0.07057032268625463 2314 64-65 -0.014599560317591909 2314 66-67 0.020294445760104196 2314 68-69 -1.5614749805020836 2314 70-71 -0.977893561782821 2314 72-73 -0.1557030850235961 2314 74-75 -0.20738046088528606 2314 76-77 -0.08495950538582875 2314 78-79 -0.015830887901444157 2314 80-81 -0.3034397667816613 2314 82-83 -0.4297176482877596 2314 84-85 -0.24408847889596785 2314 86-87 -0.2812353343243288 2314 88-89 -0.08317245047821231 2314 90-91 -0.14800692397382775 2314 92-93 0.08670058073049347 2314 94-95 -0.2195421530544941 2314 96-97 -0.20609781882932765 2314 98-99 -0.15712900856122047 2314 100-101 0.059443030242206873 2314 102-103 -0.20657708511658157 2314 104-105 -0.2221876370984326 2314 106-107 -0.1004166692953099 2314 108-109 -0.021058957600516237 2314 110-111 -0.27209036291583644 2314 112-113 -0.31048690848503213 2314 114-115 -0.09973198866569177 2314 116-117 -0.12422809182855232 2314 118-119 -0.11211952990881002 2314 120-121 -0.1928469480381949 2314 122-123 -0.1640530799603077 2314 124-125 0.3492332874969897 2315 1 -0.10740211446362125 2315 2 -0.1852760210336264 2315 3 -0.206504453506831 2315 4 -0.5476614002463513 2315 5 -0.19548407336454687 2315 6 -0.1951114213288463 2315 7 -0.30867530737495485 2315 8 -0.2791347259878876 2315 9 -0.3182286490589661 2315 10-11 -0.21182949912664384 2315 12-13 -0.29568480230158656 2315 14-15 -0.4217005485701151 2315 16-17 -0.30942059026009616 2315 18-19 -0.12296505125833335 2315 20-21 -0.01421346884890795 2315 22-23 -0.21076933860474156 2315 24-25 -0.25935419909740887 2315 26-27 -0.1952815226617517 2315 28-29 -0.17150835108929385 2315 30-31 -0.18129739257617672 2315 32-33 -0.21167649243739817 2315 34-35 -0.3362578430156873 2315 36-37 -0.26763424530518165 2315 38-39 -0.4639185024316035 2315 40-41 -0.4342444478545815 2315 42-43 -0.536673864804321 2315 44-45 -0.5093145937468719 2315 46-47 -0.48416610577140773 2315 48-49 -0.803793913607521 2315 50-51 -0.7260323119930554 2315 52-53 -0.44047826251658506 2315 54-55 -0.5357396076979519 2315 56-57 -0.6583885515813499 2315 58-59 -0.44373181633611836 2315 60-61 -0.4997361540510674 2315 62-63 -0.5044462272892183 2315 64-65 -0.40566491526233506 2315 66-67 -0.575562352002553 2315 68-69 -0.10532520441433846 2315 70-71 -0.33091415459781715 2315 72-73 -0.24831563173019333 2315 74-75 -0.2753143972449301 2315 76-77 -0.32481766465691564 2315 78-79 -0.5168322062277042 2315 80-81 -0.18223706713397902 2315 82-83 -0.1623600668957188 2315 84-85 -0.3705228254862263 2315 86-87 -0.2799421553291559 2315 88-89 -0.33300137287985265 2315 90-91 -0.2971258806925938 2315 92-93 -0.2800298431406105 2315 94-95 -0.21795431924997288 2315 96-97 -0.34215162889382 2315 98-99 -0.27588734335512655 2315 100-101 -0.0858349994315617 2315 102-103 -0.21527702237542812 2315 104-105 -0.20272670299188178 2315 106-107 0.012177791702303153 2315 108-109 0.007992482524866062 2315 110-111 0.04254940887909342 2315 112-113 -0.07055533952437543 2315 114-115 -0.12469991955389759 2315 116-117 -0.13098813358825012 2315 118-119 -0.20752164481910285 2315 120-121 -0.11243412648978435 2315 122-123 -0.10264752571714908 2315 124-125 0.08603719726149706 2316 1 -0.20133851893730537 2316 2 -0.23789267604418285 2316 3 -0.39509824298772145 2316 4 -0.1162808897756662 2316 5 -0.05464688167892717 2316 6 -0.5478515333201273 2316 7 -0.5062553652901229 2316 8 -0.781244919191117 2316 9 -0.6462156106843793 2316 10-11 -0.5638759053654709 2316 12-13 -0.7609537136312738 2316 14-15 -0.6415800557920122 2316 16-17 -0.36330217757001293 2316 18-19 -0.3656153983427899 2316 20-21 -0.2710922458271412 2316 22-23 -0.2955450189690154 2316 24-25 -0.3274891752988722 2316 26-27 -0.598945966691069 2316 28-29 -0.35977561512512324 2316 30-31 -0.44208624099889704 2316 32-33 -0.35364558753735054 2316 34-35 -0.36802524368273737 2316 36-37 -0.3339955888094366 2316 38-39 -0.2053013441241447 2316 40-41 -0.2534365791317086 2316 42-43 -0.12093749975041135 2316 44-45 -0.13966257363763646 2316 46-47 -0.2465860375638691 2316 48-49 -0.5272883585580068 2316 50-51 -0.7429557990274986 2316 52-53 -0.70608882907554 2316 54-55 -0.5039639731347307 2316 56-57 -0.557432236002871 2316 58-59 -0.4500070744558968 2316 60-61 -0.45448832149557816 2316 62-63 -0.5374921976461664 2316 64-65 -0.3379388034676367 2316 66-67 -0.42947425034275 2316 68-69 -0.3094589290635845 2316 70-71 -0.3847921395781597 2316 72-73 -0.23275845684172225 2316 74-75 -0.06645724659498953 2316 76-77 -0.2779557311425407 2316 78-79 -0.008125632954005368 2316 80-81 -0.26730247771978455 2316 82-83 0.08870763785940738 2316 84-85 -0.16846146957966823 2316 86-87 -0.2074048750776889 2316 88-89 -0.3494279957421895 2316 90-91 -0.3833136742502923 2316 92-93 -0.22407948941757638 2316 94-95 -0.29109190199419643 2316 96-97 -0.37967478029407076 2316 98-99 -0.3611390458418171 2316 100-101 -0.3081887924530271 2316 102-103 -0.566597816514129 2316 104-105 -0.3860726702096535 2316 106-107 -0.3516050042921144 2316 108-109 -0.16334100210631064 2316 110-111 -0.4415954521147256 2316 112-113 -0.2597309052316348 2316 114-115 -0.21286127510022368 2316 116-117 -0.14071596729648306 2316 118-119 -0.18491162324114896 2316 120-121 -0.17052349205264505 2316 122-123 -0.10844290207567653 2316 124-125 -0.11512846512714958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 16.0 15 134.0 16 296.0 17 507.0 18 1707.0 19 8099.0 20 28771.0 21 67954.0 22 103016.0 23 120811.0 24 117783.0 25 78962.0 26 48618.0 27 44425.0 28 35522.0 29 31739.0 30 33994.0 31 37959.0 32 46127.0 33 58895.0 34 74348.0 35 97929.0 36 143119.0 37 288407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.47872712643647 14.568899441409233 9.998407138199141 18.95396629395516 2 15.252141051419269 25.465136699207292 37.39376423453753 21.88895801483591 3 23.134529004521713 36.458373748326395 25.196357307337045 15.210739939814843 4 13.005721722533895 18.209997971599673 33.6004514211735 35.18382888469293 5 13.08549639312304 39.654954129564416 30.073144932606738 17.18640454470581 6 24.29860230965369 29.914276262679202 25.239834515205516 20.547286912461594 7 24.550807719089505 33.308193858026854 23.09063075805728 19.050367664826357 8 28.54694385415121 25.186401822020805 27.06376119874375 19.202893125084234 9 32.98948090649074 12.976861261501643 23.768155203935915 30.2655026280717 10-11 24.27896494406924 25.39461915762849 27.76406981508885 22.562346083213423 12-13 26.200023415091028 24.470982303908823 31.782957080954954 17.5460372000452 14-15 25.40128973588594 21.50043086490173 34.5858591908997 18.51242020831263 16-17 18.479407652650735 25.688328802331707 34.701198934341086 21.131064610676464 18-19 18.980245558960423 23.339604584457007 39.83774839395618 17.842401462626384 20-21 21.364466782562292 22.55584567276866 39.143089348992405 16.93659819567665 22-23 19.96768114605324 26.56261965140154 35.13358244081542 18.33611676172979 24-25 18.35110582905187 28.011964953264666 36.375214073148356 17.26171514453511 26-27 19.60157971247817 26.52744057783467 36.292713111593145 17.57826659809401 28-29 18.77172961156176 27.633514051092217 36.69471314615335 16.900043191192673 30-31 20.80501963026686 27.47366038532163 36.281702412982696 15.439617571428816 32-33 16.29455822460742 28.502083984259336 37.02368383613751 18.179673954995742 34-35 15.172276479627522 30.40986605539871 36.30489201556759 18.112965449406175 36-37 17.084342155149702 30.904049558818425 33.823988869550064 18.187619416481805 38-39 17.00888548250743 29.91121324205078 34.181676602198024 18.898224673243767 40-41 15.984246501680582 30.779901629123007 33.98389799705133 19.251953872145087 42-43 17.247528823024112 30.059701675404217 33.3486370919546 19.344132409617067 44-45 17.793018763383696 29.15788033527143 33.06224466319706 19.986856238147812 46-47 16.63908189155661 28.847106151183628 33.9021480728946 20.611663884365168 48-49 16.703227209621076 29.2551621841569 32.787642411682036 21.253968194539983 50-51 14.676367814933897 29.152152499000998 33.28447814008075 22.887001545984358 52-53 15.131771314316019 28.147692525543956 32.16661294127298 24.55392321886704 54-55 15.841568655216642 27.288927043535327 32.02949057646799 24.840013724780036 56-57 17.031331108323702 25.37937208435521 32.13374565791243 25.455551149408663 58-59 16.038116698776644 25.75865778025883 33.46995391140369 24.73327160956083 60-61 17.389882053626472 25.104546393749377 32.77774959994091 24.727821952683247 62-63 15.735837252940513 25.328632641106708 34.014963204648595 24.920566901304184 64-65 15.038386742602919 26.194195600516494 33.461981162962765 25.305436493917817 66-67 15.98171853154707 26.057763123682047 32.75852915110766 25.20198919366322 68-69 16.222948422816643 26.006780847000076 32.82608577274565 24.944184957437628 70-71 15.55322311889437 26.02393515644569 32.22312297840364 26.199718746256302 72-73 16.691862847465657 25.456049738009632 32.078912940785685 25.773174473739026 74-75 17.18412429601576 24.5198204661509 32.02405083797438 26.272004399858968 76-77 16.623761688827056 24.672426960571435 31.210682726877938 27.49312862372357 78-79 16.91856040753149 24.846985102829006 30.456056544722145 27.77839794491736 80-81 16.273760531685927 25.428686753729057 29.26386084901487 29.033691865570148 82-83 16.234894203267494 25.26250086785585 27.609999877479176 30.89260505139749 84-85 17.305091356354104 24.863984774735098 27.042236360530026 30.788687508380768 86-87 17.588362640350873 24.439796192373652 26.371857367333945 31.599983799941526 88-89 18.00131376079343 24.757413729140644 26.001504358214238 31.23976815185169 90-91 19.035007222826582 25.154974219750926 25.113345959097426 30.696672598325065 92-93 18.308602604019345 26.473708772048067 24.0150341594156 31.202654464516993 94-95 17.91321415222857 27.30010477534573 24.38991678563672 30.39676428678898 96-97 18.18706972614976 27.675663384239996 23.82760588004619 30.30966100956406 98-99 18.739551551127658 28.49283284490221 23.62657469251647 29.141040911453665 100-101 18.48272293549249 28.080910550064424 24.294168809881654 29.14219770456143 102-103 18.093927353732532 29.789401803901917 24.17232655417697 27.94434428818858 104-105 18.327131903260412 29.84436520773089 24.92372445228643 26.90477843672227 106-107 17.20675007547089 30.42959017009964 25.676574823047567 26.6870849313819 108-109 17.11826721524661 31.066963940817892 25.495047567381025 26.31972127655447 110-111 16.647817713206166 31.394993858633192 26.535094958906104 25.422093469254538 112-113 15.669403193847817 31.995076020820385 26.210863928959654 26.12465685637214 114-115 15.682608225100857 31.132086388131263 27.34901510206332 25.83629028470456 116-117 15.82806777039854 30.772895924614247 28.056471248086464 25.34256505690075 118-119 15.518045275528916 31.592879634180044 28.186936829623903 24.70213826066714 120-121 15.607655645691555 31.920452673608608 28.79218970580027 23.679701974899565 122-123 14.862456760358794 33.216552835744494 28.29686523662175 23.624125167274958 124-125 13.898848730142946 34.57787453052139 28.8962808166646 22.626995922671068 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11.0 1 8.0 2 35.0 3 74.0 4 107.0 5 166.5 6 233.5 7 364.0 8 607.5 9 900.0 10 1197.0 11 1562.5 12 2019.5 13 2569.0 14 3178.0 15 3896.0 16 4786.0 17 5841.5 18 7069.0 19 8425.0 20 9934.0 21 11511.0 22 13186.5 23 15164.0 24 17190.0 25 19596.0 26 22408.0 27 25538.0 28 29197.0 29 33292.0 30 37506.0 31 41427.0 32 43908.0 33 44148.0 34 42538.5 35 39052.0 36 34582.5 37 30197.0 38 25526.5 39 20942.5 40 17223.5 41 14315.5 42 12770.0 43 13316.0 44 15684.5 45 19220.5 46 26451.5 47 33310.0 48 48495.5 49 68639.0 50 93220.0 51 101787.0 52 79692.0 53 66280.5 54 63688.0 55 56009.0 56 44582.0 57 32034.0 58 21672.0 59 15063.0 60 9585.0 61 6539.5 62 4669.5 63 2544.5 64 989.0 65 319.0 66 197.0 67 156.0 68 134.0 69 118.0 70 108.0 71 95.0 72 88.5 73 76.5 74 55.5 75 39.0 76 26.0 77 17.0 78 9.5 79 6.5 80 7.5 81 6.0 82 3.5 83 3.5 84 2.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005717638506389461 2 0.0 3 3.4033562538032504E-4 4 0.0 5 0.0 6 0.0 7 6.806712507606501E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001803778814515723 24-25 9.529397510649102E-4 26-27 0.009291162572882873 28-29 0.006023940569231753 30-31 0.007181081695524859 32-33 0.0052411686308570056 34-35 0.0041180610671019335 36-37 0.0010210068761409752 38-39 0.0 40-41 2.7226850030426003E-4 42-43 0.0 44-45 0.0 46-47 0.002076047314819983 48-49 0.007725618696133379 50-51 0.011639478388007118 52-53 0.016914680581402157 54-55 0.017833586769929032 56-57 0.015689472330032983 58-59 0.015519304517342822 60-61 0.019909634084749016 62-63 0.009155028322730744 64-65 7.487383758367151E-4 66-67 0.0 68-69 0.0 70-71 1.3613425015213002E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 6.806712507606501E-5 86-87 5.105034380704876E-4 88-89 0.004968900130552746 90-91 0.012524351013995964 92-93 0.014736532578968077 94-95 0.01912686214637427 96-97 0.020828540273275895 98-99 0.021747446461802773 100-101 0.02419786296454111 102-103 0.021917614274492934 104-105 0.008304189259279933 106-107 0.0019399130646678528 108-109 7.827719383747477E-4 110-111 1.7016781269016252E-4 112-113 6.806712507606501E-5 114-115 6.806712507606501E-5 116-117 6.806712507606501E-5 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0694284675775863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1469138.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.2971644281355 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56310793472294 20.133926384884948 2 9.379935737123784 4.93331424808977 3 4.532010482823879 3.5753707457055985 4 2.4354388078925435 2.561805391432501 5 1.442738865758709 1.896997058985924 6 0.905792242372196 1.429186052123472 7 0.629610388936328 1.1589877546464673 8 0.48317169233345425 1.0164836352250675 9 0.3715642054649334 0.8793976506048788 >10 2.5872087843709073 13.545536396881788 >50 0.32969707198146225 6.068110067277213 >100 0.2700302615766702 14.659434350271297 >500 0.036518378225782536 6.541942411190729 >1k 0.030603429639916347 14.667279296585797 >5k 0.0010286867105854234 1.6330340566613515 >10k+ 0.0015430300658781353 5.299194499433202 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19626 1.335885396742852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13150 0.8950826947502549 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12771 0.8692852543464262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11678 0.7948878866382872 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 10643 0.72443841218456 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10489 0.713956074922846 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 6581 0.44794975012558386 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6271 0.4268489413520037 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5750 0.39138596918737384 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 5545 0.3774322085467805 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4374 0.29772560508270834 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 4372 0.29758947083255627 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 4155 0.2828189046910501 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 4045 0.275331520932683 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3966 0.26995421805167386 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3687 0.2509634901554517 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 3675 0.25014668465453893 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 3623 0.24660719415058352 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 3479 0.23680552813963016 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 3331 0.22673159362837256 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3289 0.22387277437517783 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 3283 0.22346437162472144 No Hit CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 3243 0.2207416866216788 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3153 0.214615645364833 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3114 0.21196102748686643 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 2994 0.20379297247773864 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA 2977 0.20263583135144556 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 2813 0.19147282283897088 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2809 0.19120055433866662 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 2657 0.18085435132710473 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2655 0.18071821707695263 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 2582 0.17574931694639986 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 2545 0.17323083331858546 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 2412 0.16417790568346882 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 2327 0.15839220005200327 No Hit CTGCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 2271 0.15458044104774363 RNA PCR Primer, Index 17 (95% over 21bp) CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 2166 0.14743339291475682 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 2131 0.14505104353709453 No Hit GCACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 2127 0.1447787750367903 RNA PCR Primer, Index 17 (95% over 23bp) ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 2107 0.14341743253526898 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 2081 0.1416476872832913 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.14015021053161786 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1994 0.13572584740167362 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 1976 0.13450063915030447 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 1900 0.1293275376445235 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 0.12905526914421928 No Hit GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA 1891 0.12871493351883895 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1858 0.12646871839132878 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT 1826 0.12429057038889472 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 1822 0.12401830188859045 No Hit GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACAGGCA 1732 0.11789226063174461 No Hit CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA 1725 0.11741579075621215 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC 1670 0.11367209887702856 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 1660 0.11299142762626793 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 1644 0.11190235362505088 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG 1629 0.1108813467489099 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA 1627 0.11074521249875777 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA 1625 0.11060907824860565 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 1612 0.10972420562261681 No Hit GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.10924773574708434 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1601 0.10897546724678009 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 1596 0.10863513162139976 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC 1594 0.10849899737124763 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 1577 0.10734185624495453 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.1058443794932811 No Hit CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 1536 0.10455110411683585 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA 1515 0.1031216944902385 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG 1512 0.1029174931150103 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA 1501 0.10216875473917357 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1491 0.10148808348841294 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA 1478 0.10060321086242409 No Hit CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA 1470 0.10005867386181558 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.7226850030426003E-4 0.0 2 0.0 0.0 0.0 2.7226850030426003E-4 0.0 3 0.0 0.0 0.0 3.4033562538032504E-4 0.0 4 0.0 0.0 0.0 5.445370006085201E-4 0.0 5 0.0 0.0 0.0 8.168055009127801E-4 0.0 6 0.0 0.0 0.0 8.168055009127801E-4 0.0 7 0.0 0.0 0.0 9.529397510649102E-4 0.0 8 0.0 0.0 0.0 0.0010210068761409752 0.0 9 0.0 0.0 0.0 0.0010890740012170401 0.0 10-11 0.0 0.0 0.0 0.0027907521281186655 0.0 12-13 0.0 0.0 0.0 0.006432343319688144 0.0 14-15 0.0 0.0 0.0 0.008542424197046159 0.0 16-17 0.0 0.0 0.0 0.015247036017038564 0.0 18-19 0.0 0.0 0.0 0.025491138340986347 0.0 20-21 0.0 0.0 0.0 0.049382699242685166 0.0 22-23 0.0 0.0 0.0 0.13593004877690182 0.0 24-25 0.0 0.0 0.0 0.32495245511313436 0.0 26-27 0.0 0.0 0.0 0.5768008178945749 0.0 28-29 0.0 0.0 0.0 0.9618225108873366 0.0 30-31 0.0 0.0 0.0 1.3992558901886682 0.0 32-33 0.0 0.0 0.0 2.0277196560159765 0.0 34-35 0.0 0.0 0.0 2.617657429050232 0.0 36-37 0.0 0.0 0.0 3.3696630268905983 0.0 38-39 0.0 0.0 0.0 4.229793252914294 0.0 40-41 0.0 0.0 0.0 5.483555663252874 0.0 42-43 0.0 0.0 0.0 6.498028095386546 0.0 44-45 0.0 0.0 0.0 7.377795686994687 0.0 46-47 0.0 0.0 0.0 8.243235148774314 0.0 48-49 0.0 0.0 0.0 8.954026102381125 0.0 50-51 0.0 0.0 0.0 9.610669657989924 0.0 52-53 0.0 0.0 0.0 10.359510134514252 0.0 54-55 0.0 0.0 0.0 11.042529701090027 0.0 56-57 0.0 0.0 0.0 11.712650547463888 0.0 58-59 0.0 0.0 0.0 12.416158318687557 0.0 60-61 0.0 0.0 0.0 12.998812909338673 0.0 62-63 0.0 0.0 0.0 13.568772981163104 0.0 64-65 0.0 0.0 0.0 14.173345186088714 0.0 66-67 0.0 0.0 0.0 14.744972902477507 0.0 68-69 0.0 0.0 0.0 15.37231356074106 0.0 70-71 0.0 0.0 0.0 16.07636586896534 0.0 72-73 0.0 0.0 0.0 16.696151076345448 0.0 74-75 0.0 0.0 0.0 17.279350203997176 0.0 76-77 0.0 0.0 0.0 17.86598672146524 0.0 78-79 0.0 0.0 0.0 18.3900695509884 0.0 80-81 0.0 0.0 0.0 18.97241783957668 0.0 82-83 0.0 0.0 0.0 19.763528000773242 0.0 84-85 0.0 0.0 0.0 20.305240215691107 0.0 86-87 0.0 0.0 0.0 20.841166724977505 0.0 88-89 0.0 0.0 0.0 21.353984445300576 0.0 90-91 0.0 0.0 0.0 21.854618150235034 0.0 92-93 0.0 0.0 0.0 22.304065377112295 0.0 94-95 0.0 0.0 0.0 22.771414257884558 0.0 96-97 0.0 0.0 0.0 23.228450969207792 0.0 98-99 0.0 0.0 0.0 23.710332181183794 0.0 100-101 0.0 0.0 0.0 24.25098935566298 6.806712507606501E-5 102-103 0.0 0.0 0.0 24.73293863476406 6.806712507606501E-5 104-105 0.0 0.0 0.0 25.197428696283126 6.806712507606501E-5 106-107 0.0 0.0 0.0 25.631322585080504 1.0210068761409751E-4 108-109 0.0 0.0 0.0 26.085670644963237 1.3613425015213002E-4 110-111 0.0 0.0 0.0 26.520143104323758 1.3613425015213002E-4 112-113 0.0 0.0 0.0 26.983816360341915 1.3613425015213002E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 840 0.0 85.70051 1 GTATAAC 480 0.0 80.56557 1 TACAACG 365 0.0 76.60944 2 GACCGTT 195 0.0 76.27509 7 ATAACGC 525 0.0 73.65995 3 GGTAATC 105 0.0 73.65994 1 CATAGTA 1010 0.0 73.6319 3 GCATAGT 1000 0.0 73.17832 2 ATAGTAC 1030 0.0 72.20215 4 AGTGTAC 670 0.0 71.0383 3 GAGTTCT 700 0.0 70.543564 1 AGTTCTT 710 0.0 69.549995 2 AGATTAC 1130 0.0 68.97159 2 TAGGACC 220 0.0 67.60747 4 TTAGGAC 225 0.0 66.10508 3 GGTTTCA 795 0.0 65.85563 1 GTACAAC 430 0.0 65.02895 1 TGAGTAC 275 0.0 64.90317 5 TAGTACT 1150 0.0 64.15067 5 CTGAGTA 280 0.0 63.744183 4 >>END_MODULE