FastQCFastQC Report
Fri 10 Feb 2017
SRR3557199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991308
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170990.8586818312385627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86810.4359446152980855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76360.38346654560720894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70780.3554447629397361No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT60240.3025147290122874No Hit
GTATTACTGTATGCAAAGTATGGAAGATCCTCGGACGTTCGGTGGAGGCA56350.2829798303426692No Hit
CAGTAATACATTGCAGCATCATCTTCCTCCACAGGATCGATGGTGAGAGT55150.27695364052170734No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54580.27409120035675044No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC50740.25480739292967236No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT50730.2547571746811644No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG44850.22522884455845102No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38810.19489702245960946No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT38520.193440693252877No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT38050.19108043557300025No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36920.18540577349159446No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA35240.1769691077422478No Hit
CTCTAGGACAGAGGGCCACCATATCCTGCCAAGCCAGCGAAAGTGTCAGT33900.17023986244217368No Hit
GTAATACATTGCAGCATCATCTTCCTCCACAGGATCGATGGTGAGAGTGA33090.1661721843130244No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT32840.164916728100324No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32660.1640127996271797No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32600.16371149013613165No Hit
AAATCAAACGGGCTGATGCTGCACCAACTGTATCCATCTTCCCACCATCC32380.16260668866895528No Hit
ATGTATTACTGTATGCAAAGTATGGAAGATCCTCGGACGTTCGGTGGAGG31560.15848879229129798No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT31200.1566809353450094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31120.1562791893569453No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT30640.1538687134285605No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGG30560.1534669674404964No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT29940.15035343603299942No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT29780.14954994405687116No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA29350.14739055937102646No Hit
GTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTG28930.1452813929336898No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC28780.1445281192060696No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC27740.13930542136123591No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA27530.13825083814256758No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG27500.13810018339704355No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27470.1379495286515195No Hit
GTGGAGGCACCAAGCTGGAAATAAAACGGGCTGATGCTGCACCAACTGTA27130.13624210820224697No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA27110.13614167170523092No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA26350.1323250848186217No Hit
GTATGGAAGATCCTCGGACGTTCGGTGGAGGCACCAAGCTGGAAATCAAA25850.12981417239322093No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC25590.12850849793201252No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT24670.12388841906927506No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG24480.12293427234762278No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG24040.12072466941327006No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT23880.11992117743714183No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG23180.11640590004158072No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA23090.11595393580500857No Hit
GTGCAAAGACTCACTTTATTGAATATTTTCTGCAAATATTAGCATGATAA22770.11434695185275207No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT22040.11068101971166691No Hit
GGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTC21960.11027927372360279No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG21560.10827054378328214No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21370.10731639706162985No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.10410242915711683No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC20400.10244522695635232No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT20210.10149108023469999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2350.070.819792
GTACAAC2600.066.904411
TAACGCA4050.060.171854
ACAACGC2800.059.4380463
TCACGCA2150.058.0557634
AGTGTAC4250.054.543143
CGCATAG4050.050.356321
AGATTAC6850.048.5916822
TATCACG2600.048.007652
GTATAAC5350.044.84731
TAGTACT5050.044.7256555
GAGTTCT5850.044.090261
ATCACGC2850.043.7964553
GATTACT7650.042.7332343
ATAACGC6300.041.5122833
GTATCAC3500.041.1313821
ATAGTAC5250.040.757524
AAACGCA5100.039.6253625
GCATAGT5250.039.6253622
AGTTCTT6900.037.04112
Produced by FastQC (version 0.11.5)